text
stringlengths 10
10.1k
| source
stringclasses 1
value |
|---|---|
SE to air pollution is associated with increased cardiovascular morbidity although the underlying mechanisms are unclear V1 dysfunction reduces arterial compliance and increases CE arterial pressure and left ventricular afterload we determined the effect of DE SE on arterial compliance using a validated noninvasive measure of arterial stiffness | MEDAL |
in this study an amphotropic retrovirus has been used to efficiently transduce normal human nf and scleroderma systemic sclerosis ssc dermal fibroblasts sscf with a sequence encoding a temperaturesensitive mutant of the sv C1 t antigen tsau from the primary outgrowths of skin explants cultures were generated whose growth was stringently temperaturedependent when grown at a low permissive temperature degrees c both normal and ssctransduced cells continuously divided with similar doubling times whereas at a high NPT degrees c division of both the nf and sscf cells was rapidly arrested these cells have been passaged more than times have the typical morphological appearance of fibroblasts and have retained an anchoragedependent phenotype the transduced normal cells tstnf synthesized the matrix molecules collagen and fibronectin and expressed phenotypic antigens characteristic of their nontransduced counterparts including mhc class i vla beta cd hermes cd vla alpha cdd icam cd and lfa cd and the cell surface ectoenzymes NEP cd aminopeptidase n cd and dipeptidyl peptidase iv cd analysis of the transduced ssc fibroblasts tstsscf showed that these cells exhibited certain L1 features of the ssc pathology notably the abnormally high synthesis of type i collagen increased expression of icam and depressed levels of cd moreover these phenotypic characteristics were retained even after prolonged culture in vitro the tstsscf cells also retained their responsiveness to cytokines since interferongamma ifngamma and RT necrosis factoralpha TNF both produced a marked increase in icam expression our findings show that infection of sscf with the sv tst antigen extends the life span of these cells and does not ablate their abnormal phenotypic and functional characteristics | MEDAL |
radiation therapy for advanced prostate CA has doselimiting complications and often results in limited tumor control a combination of radiation and taxol a potential radiation sensitizer may enhance therapeutic efficacy at currently used individual doses human prostatic carcinoma lines in vitro and dunning rat prostatic adenocarcinoma in vivo were treated with taxol and radiation individually and in combination cytotoxicity of taxol was comparable between androgen CS and insensitive lines with growth inhibition at to nm combining agents increased cytotoxicity with a dose modifying ratio of at survival flow cytometry showed an enhancement of radiation toxicity associated with taxolinduced cell cycle phase arrest at gm injection of taxol mgkgday x radiation dose fractionation gyday x and their combination significantly delayed dunning RT growth adverse SE were minimal the results imply that combination of these agents may have clinical potential in prostate CA treatment | MEDAL |
salicylic acid has been widely used in the topical treatment of PS chemically it is closely related to paraaminobenzoic acid following in vitro studies indicating that salicylic acid might exhibit relevant uvb absorption we found that salicylic acid had a clinically pronounced filter effect when applied prior to uvb SE the duration of photoprotection after application was more than h sometimes exceeding h in a prospective randomized doubleblind leftright comparison study in patients with PS between emollients with and without salicylic acid salicylic acid was shown to decrease the clearing rate significantly | MEDAL |
nuclear magnetic resonance nmr spectroscopy is a powerful technique for the study of the structure dynamics and folding of proteins in solution it is particularly powerful when applied to dynamic or flexible systems such as partially folded MG states of proteins which are not usually amenable to xray crystallography in this article nmr methods suitable for the detailed characterisation of molten globule states are described the TPS method used to T0 the MG is determined by the SQ of the nmr spectrum obtained molten globules are characterised by significant levels of AA structure sitespecific hydrogendeuterium exchange experiments can be used to identify residues located in regions of AA structure in the molten globule if spectra characterised by sharp peaks are observed for the molten globule then information about AA structure can be obtained by analysis of halpha calpha cbeta and co delta this can be supplemented by n EC50 studies for molten globules characterised by extremely broad peaks nedited nmr experiments carried out in increasing concentrations of denaturants can be used to study the relative stabilities of different regions of structure examples of the application of these methods to the study of the low ph molten globule states of alphalactalbumin and apomyoglobin are presented | MEDAL |
the purpose of this study was to assess whether the same principles to evaluate renal SPK by fineneedle aspiration fna for AAR could be applied to pancreatic allograft between ultrasoundguided fnas on patients with pancreatic allograft were performed and thinprep made the percentage of lymphocytes lymphoblasts monocytes eosinophils plasma cells immunoblasts and macrophages were calculated simultaneous IP smtarsal stress fractures additionally patients tend to have long disease histories with diverse medication reconstructive surgery and osteoporosis if such patients experience sudden pain tenderness or swelling in the ankle region AS fractures should be suspected and necessary examinations performed | MEDAL |
as a basis for the quantitation of the effect of antianaerobic drugs in vivo an experimental thigh infection in mice was developed in which coinoculation of bacteroides fragilis and escherichia coli is obligatory for proliferation of bact fragilis the GABA effect of metronidazole and tinidazole on the proliferation of bact fragilis was assessed in this MM both metronidazole and tinidazole showed a significant doseeffect relationship with respect to the outgrowth of bact fragilis but not that of e coli the effect of both drugs on bact fragilis was similar and at all dosage C2 a significant rho was found between the numbers of bact fragilis and e coli | MEDAL |
this article reports the results of a study that explored whether outdoor recreational Az are sufficient in istanbul in terms of their surface area and facility characteristics all the municipalities in subprovinces of istanbul were sent a survey in and asked to prepare a list of their outdoor recreational areas including their names addresses size and facilities all the data collected from the municipalities were used to create a gisbased inventory by using gis and remote sensing as the study revealed the outdoor recreational Az in istanbul are far behind meeting the recreational needs of the residents in terms of area per person and facility characteristics there are areas which were dedicated to outdoor recreational MICs in istanbul total surface area of all these outdoor recreational areas is sq kilometers this means m recreational area per person in the city this value is very low when compared to that of many cities in europe and usa as the T0 also revealed the majority of outdoor recreational areas in istanbul are poor in facility majority of the existing outdoor recreational Az are small and do not provide the public with many opportunities to engage in different outdoor activities a more sustainable and efficient recreational plan is needed in istanbul to meet the various recreational needs of its residents | MEDAL |
the purpose of this article was to assess validity of the regional disabilities of arm shoulder and hand quickdash and shortform sf for surveillance purpose we compared the predictive discriminate and concurrent validity of the quickdash and sf among workers with specific clinical diagnoses of neck or upper extremity MSK disorders uemsds and workers with symptoms only compared to those with PS only the odds of being any neck or uemsd case were confidence interval ci and ci with every point increase in quickdash disability and physical component scale pcs scores respectively the clinical cases had significantly higher quickdash disability vs p and lower pcs scores vs p than those with symptom only the quickdash disability scores were moderately correlated with the pcs scores rho among the clinical cases either quickdash or pcs can be used as a simple surveillance tool in an AS working population | MEDAL |
acute experiments on anesthetized and immobilized cats using extracellular derivation of neuronal responses and field potentials demonstrated the predominant localization of somatic radial nerve responses in the mediocentral and of visceral PSN responses in the centrolateral regions of the ventral posterolateral thalamic nucleus both similarities and differences were found in patterns of neuronal responses to IP stimuli of different types a quite high degree of convergence of somatovisceral impulsation was found in the CE part of the nucleus compared with its medial and lateral regions predominance of comparatively longlatency convergent SN over shortlatency was observed the GABA character of somatovisceral interaction and the more effective inhibitory influence of conditioning stimulation of the radial nerve on testresponse of splanchnic origin were demonstrated | MEDAL |
the sensory and physiochemical properties of sausages with varying fat and salt levels were investigated twenty eight sausages were produced with varying concentrations of fat ww and salt ww sausages were assessed instrumentally for colour moisture fat cooking loss and texture profile analysis consumers n evaluated each product in duplicate for colour texture tenderness juiciness salt taste meat flavour offflavour and overall acceptability using a hedonic scale lowering fat produced products which consumers rated as less dark in colour tougher less juicy and taste less salty than higher fat products however no significant preferred sample was found amongst consumers salt reduction in products produced sausages which consumers rated as paler in colour more tender and with greater meat flavour than higher salt containing products the sausages containing and salt were significantly p found to be more acceptable to consumers than other salt C2 | MEDAL |
multiplelentigines mlleopard multiple lentigines electrocardiographicconduction abnormalities ocular hypertelorism PS abnormal genitalia retardation of growth and sensorineural deafness syndrome is an autosomal dominant conditioncharacterized by lentigines and café au lait spots facial anomalies cardiac defectsthat shares several clinical features with noonan syndrome ns we screened nine patients with mlleopard syndrome including a motherdaughter pair and two children with ns who had multiple café au lait spots for mutations in the ns gene ptpn and found in of patients one of two new missense mutations in exon or exon both mutations affect the ptpn phosphotyrosine phosphatase domain which is involved in of the ns ptpn mutations the study demonstrates that mlleopard syndrome and ns are allelic disorders the detected mutations suggest that distinct molecular and pathogenetic mechanisms cause the peculiar cutaneous manifestations of the mlleopardsyndrome subtype of ns | MEDAL |
hypoallergenic formulas are processed by enzymatic hydrolysis of different protein sources such as bovine caseinwhey and soy followed by further processing such as heat treatment andor ultrafiltration or they are based on CAA mixtures the products have been classified according to the degree of protein hydrolysis as extensively or partially hydrolysed protein products product properties may be characterized by PSA techniques and reduction of allergenicity may be assessed in vitro with various immunological methods and in vivo with skin prick tests patch tests and challenge tests in vitro tests do not predict the allergenic effects in humans and at present there is no evidence of a specific threshold of immunogenic protein only pure amino acid mixtures are considered to be nonallergenic other hypoallergenic products contain residual allergenicity at present the potential of a product for treatment and prevention of food allergy can only be determined by PCTs using scientifically AGA standards it has been recommended that dietary products for treatment of CM protein allergy in infants should be tolerated by at least with confidence of infants with documented cows milk protein allergy some extensively hydrolysed products and aminoacidbased products have met these criteria formulas intended for prevention should have a very low if any allergenic activity until otherwise proven so far there are no firm criteria available for the design of hypoallergenic foods for prevention newborns included in prevention studies should be from highrisk families they should be randomized at birth and fed the formula when supplements are needed for at least the first months of life followup should be at least months and children should be investigated when symptoms appear validated clinical criteria including controlled food challenges should be used for diagnosis infants fed formulas that claim to prevent or delay allergy should have a statistically significant lower prevalence of allergy when compared with infants fed a standard CMF FF highrisk infants a documented hypoallergenic formula combined with avoidance of solid foods during the first months reduces the cumulative incidence of CM protein allergy and atopic dermatitis as compared with a standard cowsmilkbased formula partially hydrolysed formulas may have an effect though it seems to be less than that of extensively hydrolysed formulas at present thus if exclusive BF for months is not possible in highrisk infants a documented hypoallergenic formula and avoidance of solid foods are recommended for the first months of life | MEDAL |
kinetic studies of antigen distribution to various organs were performed throughout the course of experimental immune complex ic glomerulonephritis induced in rats by using a paired radiolabel technique and histologic observations the authors found massive amounts of antigen within both the lungs and the livers of these rats before the OD of glomerulonephritis however the rate of antigen disappearance from the lung exceeded that from the liver polymorphonuclear WBCs pmns handled almost all the antigen administered presumably in the form of ics electron microscopy yielded little evidence that KCs or monocytes phagocytosed these ics T3 glomerulonephritis developed only minimal amounts of antigen were evident in the lungs and livers these observations indicate that the mononuclear phagocyte system mps does not participate in the processing of ics as previously believed but that pmns dispose of nearly all the ics formed in vivo in this MM of glomerulonephritis | MEDAL |
we report successful surgical management of a circumscribed choroidal hemangioma with exudative retinal detachment refractory to transpupillary thermotherapy ttt a yearold man with symptomatic serous macular detachment in the left eye snellen acuity AA to a paramacular choroidal hemangioma was treated with ttt the nonresponsive detachment was subsequently managed by vitrectomy endophotocoagulation and siliconoil tamponade it resulted in complete resolution of the RT and the detachment silicon oil was removed at four months visual acuity improved to by the last followup visit at months without any recurrence | MEDAL |
fatigue is common among persons living with hiv plwh and risk factors for obstructive sleep apnea osa such as older age and obesity are increasingly prevalent studies of osa among plwh are lacking so we aimed to characterize osa symptoms and associated clinical consequences eg fatigue among a contemporary population of plwh selfadministered surveys containing items that included selfreported snoring witnessed apneas estimated sleep duration the epworth sleepiness score ess and the facitfatigue score were mailed to plwh receiving care at an urban hiv clinic clinicaldemographic data were collected from the medical record multivariable linear regression models were created to study relationships between fatigue clinical variables and osa symptoms of surveys responded eight patients had known osa among those without known osa mean respondent characteristics included age years male african american caucasian bmi kgm duration of hiv diagnosis years on ARV therapy and with hiv rna copiesml reported snoring reported witnessed apneas and had EDS witnessed apnea was the strongest independent predictor of fatigue lower facitfatigue score β p this difference of points exceeds the accepted minimal clinically MID of points other predictors included opioid use β p depression β p antidepressant use β p and sleep duration h β p our data strongly support the need for increased efforts directed at osa screening and treatment in plwh | MEDAL |
night myopia which is a shift in refraction with light level has been widely studied but still lacks a complete understanding we used a new infrared openview binocular hartmannshack wave front sensor to quantify night myopia under monocular and natural binocular viewing conditions both eyes accommodative response aberrations pupil diameter and convergence were simultaneously measured at light levels ranging from photopic to scotopic conditions to total darkness for monocular vision reducing the CS- luminance resulted in a progression of the accommodative state that tends toward the subjects dark focus or tonic accommodation and a NC in convergence following the induced accommodative error most subjects presented a myopic shift of accommodation that was mitigated in binocular vision the impact of spherical aberration on the focus shift was relatively small our results in monocular conditions support the hypothesis that night myopia has an accommodative origin as the eye progressively changes its accommodation state with decreasing luminance toward its resting state in total darkness on the other hand binocularity restrains night myopia possibly by using fusional convergence as an additional accommodative cue thus reducing the potential impact of night myopia on vision at low CS levels | MEDAL |
there is a nonlinear interaction of binaural stimulation on ABR potentials in both human and animals the interaction takes the form of the binaurally evoked abr being of smaller amplitude than the sum of the monaurally evoked abrs in the guinea pig this interaction occurs at the time of components p n and p in order to investigate the generator CS of binaural interaction in the abr various lesions were made in the BS AEP system in guinea pigs the effects of those lesions on binaural interaction were as follows unilateral lesion of LL or BL lesions of the VPI colliculi had no significant effect on Bi transection of the lateral lemnisci bilaterally was associated with a loss of the component of binaural interaction associated in time with n a lesion just lateral to the lateral superior olivary complex resulted in an attenuation of the component of Bi associated in time with p complete section of the decussating SF of the TB or a CR unilateral lesion of the superior olivary complex led to a loss of all components of binaural interaction these results suggest that binaural interaction in the guinea pig abr requires the integrity of several distinct portions of the brainstem AEP pathway ie both lateral lemnisci are required for the interaction occurring at the time of n the BS just lateral to the LSO participates in the interaction at the time of p the TB and superior olivary nucleus are required for binaural interaction at p n and p | MEDAL |
in our previous studies the effects of effusion and pressure on sound transmission were investigated separately the aim of this T0 is to investigate the combined effect of fluid and pressure on ME function an OM with effusion MM was created by injecting saline solution and air pressure simultaneously into the middle ear of human temporal bones Tty membrane displacement in response to db spl sound input was measured by a laser vibrometer and the compliance of the ME was measured by a tympanometer the movement of the Tty membrane at the umbo was reduced up to db by the combination of fluid and pressure in the middle ear over the auditory frequency range the fluid and pressure effects on the umbo movement in the fluidpressure combination are not additive the combined effect of fluid and pressure on the umbo movement is different compared with that of only fluid or pressure NC in the ME negative pressure in fluidpressure combination had more effect on middle ear CF than positive pressure tympanometry can detect the middle ear pressure of the fluidpressure combination this T0 provides quantitative information for analysis of the combined effect of fluid and pressure on Tty membrane movement | MEDAL |
the major effector cells for cellular adaptive immunity are cd cytotoxic t lymphocytes ctls which can recognize and kill virusinfected cells and RT cells although ctls exhibit strong cytolytic activity against target cells in vitro a number of studies have demonstrated that their CF is often impaired within tumors nevertheless ctls can regain their cytotoxic ability after escaping from the tumor environment suggesting that the milieu created by tumors may affect the CF of ctls as for the tumor environment the pathophysiological situation present in vivo has been shown to differ from in vitro exp conditions in particular low ph and hypoxia are the most important microenvironmental AF within growing tumors in the present T0 to determine the effect of these factors on ctl CF in vivo we examined the cytolytic activity of ctls against their targets using mu ctl lines and the induction of these cells from memory cells under low ph or hypoxic conditions using antigenprimed SP cells the results indicated that both cytotoxic activity and the induction of functional ctls were markedly inhibited under low ph in contrast in hypoxic conditions although cytotoxic activity was almost unchanged the induction of ctls in vitro showed a slight enhancement which was completely abrogated in low ph conditions therefore antigenspecific ctl functions may be more vulnerable to low ph than to the oxygen concentration in vivo the findings shown here provide new therapeutic approaches for controlling RT growth by retaining ctl cytotoxicity through the maintenance of higher ph conditions | MEDAL |
the s peplomer gene sequences of strains of avian coronavirus infectious bronchitis virus ibv from north america europe and australia were compared to identify common and unique regions for possible diagnostic applications s sequences that were conserved among serotypes and sequences that were variable between serotypes were identified based on conserved s gene sequences GA degenerate oligonucleotide primers were designed that amplified ibv genomic rna by the reverse transcriptase polymerase chain reaction rtpcr procedure regardless of serotype primers specific for ibv serotypes massachusetts CT arkansas jmk delaware de and california ca were designed from regions of the s gene exhibiting extensive sequence hypervariability the ability to identify these six serotypes of ibv by rtpcr was demonstrated by testing the serotypespecific primers on a panel of unknown samples that included REF strains and field isolates previously characterized by virus neutralization vn the use of serotypespecific primers in rtpcr provides a rapid and accurate means of identifying ibv | MEDAL |
the cadherinmediated cellcell adhesion system plays a critical role in normal development and morphogenesis inactivation of this system is thought to be responsible for cancer invasion and metastasis a HepG2 hcc cell L1 kyn was observed to have great potential for intrahepatic metastasis when orthotopically implanted into the liver of scid mice in vitro cultures of kyn cells showed that they formed trabecular structures in suspension but lost tight cellcell adhesion and became scattered when attached to a substratum such as collagen or fibronectin in response to adhesion to the substratum subcellular colocalization of EC and AFs were shown to be reduced and a significant amount of alphacatenin was dissociated from the ecadherincatenin complex in kyn cells these changes of cellcell adhesion were blocked by GABA monoclonal antibodies against beta and CD18 we found that csrc was coimmunoprecipitated with ecadherincatenin complex and was tyrosinedephosphorylated and activated in the adherent cells the IMT dephosphorylation of csrc was induced by cell adhesion to the substratum and inhibited by addition of GABA monoclonal antibodies against beta and beta integrins these findings indicate that integrinmediated cellsubstratum adhesion inhibits cadherinmediated cellcell adhesion possibly through csrc activation and suggest that this crosstalk mediates transient inactivation of the cadherin system and plays an important role in intrahepatic metastasis of human hcc modulation of this interaction might provide a new approach to prevent metastasis and recurrence of hcc | MEDAL |
muscle growth is regulated by the homeostatic balance of the biosynthesis and Kd of muscle proteins to elucidate the molecular interactions among diet pig TT and physiological stage we examined the effect of dietary protein concentration pig TT and physiological stages on CAA aa pools protein deposition and related signaling pathways in different types of skeletal muscles the study used landrace pigs and purebred bama minipigs assigned to each of dietary treatments lowergb chinese conventional diet or highernrc national research councilprotein diet diets were fed from weeks of age to respective market weights of each TT samples of biceps femoris muscle bfm type i and LD muscle ldm type ii were collected at nursery growing and finishing phases according to the physiological stage of each TT to determine the aa concentrations mrna C2 for growthrelated genes in muscles and protein abundances of mechanistic TOR mtor signaling pathway our data showed that the concentrations of most aas in ldm and bfm of pigs increased p gradually with increasing age bama minipigs had generally higher p muscle concentrations of flavorrelated aa including met phe tyr pro and ser compared with landrace pigs the mrna C2 for myogenic determining factor myogenin myocytespecific enhancer binding factor a and myostatin of bama minipigs were higher p than those of landrace pigs while total and phosphorylated protein C2 for protein kinase b mtor and p ribosomal protein s kinases psk and ratios of pmtormtor paktakt and ppskpsk were lower p there was a significant pig genotypedependent effect of dietary protein on the levels for mtor and psk when compared with the higher proteinnrc diet the lower proteingb diet increased p the C2 for mtor and psk in bama minipigs but repressed p the level for psk in landrace pigs the higher proteinnrc diet increased ratio of pmtormtor in landrace pigs these findings indicated that the dynamic consequences of aa profile and protein deposition in muscle tissues are the concerted effort of distinctive TT nutrient status age and muscle type our results provide valuable information for animal FF strategy | MEDAL |
CMT disease caused by mutations of the myelin protein zero gene demonstrates considerable phenotypical R2 we describe a yearold female with a peripheral neuropathy with demyelinating and axonal features pes cavus and pupillary lightnear dissociation she was heterozygous for two mutations in the myelin protein zero gene histyr and valphe both present on the same allele our patient shows a less severe phenotype than previously described patients with a hisarg mutation multiple mutations in the myelin protein zero gene as well as charcotmarietooth with pupillary abnormalitieres iterative techniques such as the LM lm algorithm considering the nonlinear nature of the inverse problem and the low signal to noise ratio inherent in eeg signals a backpropagation neural network bpn has been recently proposed as a solution the technique has not been properly compared with classical techniques such as the lm method or with more recent neural network techniques such as the radial basis function rbf network in this paper we provide improved strategies based on bpn and consider rbf networks in solving the inverse problem we compare the performances of bpn rbf and a hybrid technique with that of the classical lm method | MEDAL |
lymphocyte activation may be involved in interstitial inflammatory processes in the lungs we analyzed lymphocytes in BAL lavage fluid obtained from patients with idiopathic bronchiole longterm results of cases of thyrotoxic HR disease thd treated with i were analysed of patients have been followed for years for years for years for years and for years the mean period of follow up was years of the patients did not respond to antithyroid drug atd therapy before i therapy all the patients suffered from one or more of cardiac abnormalities caused by HT such as paroxysmal or permanent atrial fibrillation cardiac enlargement CF frequent atrial or ventricular extrasystoles AP and so on all patients were given individualized therapeutic i dose of mbq per gram of thyroid weight the total i dose for patients was mbq to mbq the longterm results showed that cases were cured and cases achieved pCR the total effective rate was percent there was no relapse of hyperthyroidism except that cases of early HT and of delayed hypothyroidism were diagnosed there were no other complications the incidence of postradioiodine HT at years was percent and at years percent our results indicate that i is a simple safe economic and effective treatment for thd i.c.v. with atd for hyperthyroidism with cardiac complications is not necessary i should be considered as the treatment of first choice for HT with cardiac CA | MEDAL |
radical retropubic prostatectomy rrp has resulted in substantial BL and the frequent need for rat blood transfusion in this study the efficacy of AB transfusion from medical and financial perspectives was evaluated in patients undergoing rrp | MEDAL |
neurodegeneration in parkinsons disease pd is accompanied by a local immune reaction in the affected brain regions it is well established that AS is directly implicated in the pathogenesis of pd OD of the disease is often associated with changes of expression and cellular compartmentalisation of this protein moreover its oligomers or protofibrils are often released to the csf and plasma of patients aggregated alphasynuclein can trigger the activation of microglia however its capacity to induce production of TPS autoantibodies aab has not been assessed in this T0 we examined the presence of aab against synuclein family members in the peripheral blood serum of pd patients and control individuals presence of aab against betasynuclein or gammasynuclein showed no association with pd multiepitopic aab against alphasynuclein were detected in of all patients tested and their presence strongly correlated with an inherited mode of the disease but not with other diseaserelated factors the frequency of the presence of aab in the studied group of patients with sporadic form of pd was not significantly different from the frequency in the control group but very high proportion of patients with familial form of the disease were positive for aab ace of brownian motion of a PLT on its flow characteristics near a surface in the creeping flow regime two important characterizations were done in this regard quantification of the platelets ability to contact the surface by virtue of the brownian forces and torques acting on it and determination of the relative importance of brownian motion in promoting surface encounters in the presence of shear flow we determined the peclet number for a PLT undergoing brownian motion in shear flow which could be expressed as a simple linear CF of height of the platelet centroid h from the surface pe PLT h for h microm our results demonstrate that at timescales relevant to shear flow in blood brownian motion plays an insignificant role in influencing PLT motion or creating further opportunities for plateletsurface contact the platelet peclet number at shear rates s is large enough to neglect PLT brownian motion in computational modeling of flow in arteries and arterioles for most practical purposes even at very close distances from the surface we also conducted adhesive dynamics simulations to determine the effects of PLT brownian motion on gpibalphavwfa singlebond dissociation dynamics brownian motion was found to have little effect on bond lifetime and caused minimal bond stressing as bond rupture forces were calculated to be less than pn we conclude from our results that for the case of plateletshaped cells brownian motion is not expected to play an important role in influencing flow characteristics plateletsurface contact frequency and dissociative IB phenomena under flow at physiological shear rates s | MEDAL |
ninetynine patients who had never previously taken inhaled CS were enrolled in a randomized singleblind parallel T0 the aim of which was to compare the tau and safety of FLU inhalation mcg twice daily with beclomethasone dipropionate inhaler mcg four times daily for the treatment of chronic asthma the treatment period was for weeks the patients were examined clinically at entry week and week and both treatment groups showed a marked improvement in almost all parameters during the course of the T0 flunisolide was statistically significantly superior to beclomethasone dipropionate for wheezing at week coughing at week and chest tightness at weeks and the number of asthma attacks per day decreased significantly more with FLU treatment than with beclomethasone dipropionate the overall DUE of tau by both doctors and patients also showed flunisolide to be superior to beclomethasone dipropionate in several other parameters there was a trend shown favouring flunisolide and beclomethasone dipropionate did not show a superiority over flunisolide in any efficacy parameter both drugs were welltolerated with unpleasant taste being the most frequent complaint in the flunisolide group no patient in either group withdrew from the study because of adverse events in this T0 flunisolide inhaler was more effective than beclomethasone dipropionate inhaler for the treatment of chronic asthma exhibited by patients who had never been treated with inhaled steroids | MEDAL |
both midsubstance ACL ligament acl injury and tibial spine avulsion fracture occur in the skeletally immature knee the purpose of this T0 was to determine whether there are differences in skeletal maturation or notch parameters between these two groups a retrospective casecontrol study of skeletally immature patients with tibial spine fractures compared with age and sexmatched skeletally immature patients with midsubstance acl injuries was performed bone age and notch width index were determined from preoperative radiographs there were no significant differences between the tibial spine fracture group and the acl injury group with respect to skeletal maturation comparing BA to chronological age vs years p the acl injury group had narrower notch indices than the tibial spine fracture group s the results of a week FF study in rats with grain from roundup ready corn which is tolerant to the herbicide glyphosate herbicide tolerance was accomplished through the introduction of cp epsps coding sequences into the corn genome for in planta production of cp epsps enzymes unlike related corn epsps enzymes cp epsps enzymes are not inhibited by the herbicide glyphosate purina testdiets formulated RR corn grain into rodent diets at levels of and ww the responses of rats fed diets containing roundup ready corn grain were compared to that of rats fed diets containing nontransgenic grain controls all diets were nutritionally balanced and conformed to purina mills inc specifications for certified labdiet there were rats in the study divided into CG of ratssexgroup overall health body weight food consumption clinical pathology parameters hematology blood chemistry UA organ weights gross and microscopic appearance of tissues were comparable between groups fed diets containing RR and control corn grain this study complements extensive agronomic compositional and farm animal FF studies with roundup ready corn grain confirming it is as safe and nutritious as existing commercial corn hybrids | MEDAL |
this T0 is a continuation of our research of phenomenology and mechanisms of MS ms and the relation of this phenomenon to features of sleepwake cycle swc changes it presents data about the effect of ms on swc in dayold IN rats and rats exposed previously to prenatal hypoxia on days and of gestation in all CG of animals ms was shown to reduce significantly the waking time and to increase the time of PS ps for the first time it became possible to reveal a connection between hypothalamic mechanisms controlling ms and swc and to suggest the role of this connection in mechanisms of development of the sopite syndrome which may be the only manifestation of ms in some animals and man the results obtained demonstrate that hypoxia on day of gestation has a greater damaging effect on the thalamocortical sleepregulating structures than hypoxia on day when only hypothalamichippocampal systems controlling slowwave sleep were found disturbed against this BG ms substantially suppresses the activity of the BB excitatory systems which provide wake fulness maintenance it appears to be the ARAS and enhances the work of those activating systems that control ps it is in rats exposed to preliminary hypoxia on day of gesta tion where it was possible to show the role of the evolutionary young thalamocortical system in the control of ps | MEDAL |
to develop a model synthesis method for parsimoniously modeling geneenvironmental interactions gei associated with clinical outcomes and phenotypes the ambrosia MM synthesis approach utilizes the kway interaction information kwii an informationtheoretic metric capable of identifying VL combinations associated with gei for model synthesis ambrosia considers relevance of combinations to the phenotype it precludes entry of combinations with redundant information and penalizes for unjustifiable complexity each step is kwii based the performance and power of ambrosia were evaluated with simulations and genetic association workshop gaw data sets of rheumatoid ICA ra ambrosia identified parsimonious models in data sets containing multiple interactions with linkage disequilibrium present for the gaw data set containing singlenucleotide polymorphisms the parsimonious ambrosia model identified nine raassociated combinations with power ambrosia was compared with multifactor dimensionality reduction across several diverse models and had satisfactory SP software source code is available from httpwwwcsebuffaloedudbgroupbioinformaticsresourceshtml ambrosia is a promising method for gei MM synthesis | MEDAL |
a cysteine proteinase of kda cp of TV is known to play a role in cytoadherence of the parasite to host cells however the cp activity in clinical isolates from symptomatic and asymptomatic patients has not been analyzed in the present study cp was detected in fresh and longterm SC maintained t vaginalis isolates each from symptomatic and asymptomatic women by ATP gel PACE and immunoblotting though cp was detected in all the fresh isolates from symptomatic and asymptomatic women the intensity of cp band was significantly higher in isolates from symptomatic as compared to asymptomatic women indicating higher expression in former cp was found in all the LT cultured isolates from symptomatic whereas only in of asymptomatic women indicating that cp expression is a more SD characteristic of symptomatic isolates the isolates from symptomatic women demonstrated significantly higher cytoadherence to vecs as compared to asymptomatic women in both the types of isolates this cytoadherence was inhibited significantly by cp specific hyperimmune serum these results confirm that cp is an important virulence factor of t vaginalis and has an important role in cytoadherence to vecs and thus has a role in pathogenesis of trichomoniasis | MEDAL |
antipsychotics have proven efficacy for treating patients with earlyonset schizophrenia and aripiprazole olanzapine quetiapine and risperidone have fda approval for treating adolescents aged to years children and adolescents appear to have a higher risk than adults for experiencing AEs such as extrapyramidal symptoms prolactin elevation weight gain and metabolic effects when taking antipsychotic medications pediatric studies indicate that adverse effect profiles differ among commonly used antipsychotics clinicians should consider the differing adverse effect profiles when choosing medications for young patients | MEDAL |
supplementary material the nucleotide sequence of the ribosomal ETS ets region of stylosanthes mexicana was determined and used to evaluate its potential for examination of intra and interspecific relationships in stylosanthes as compared to the use of the internal transcribed spacer its region the entire ets region comprises bp and is compoels contributed by RT stroma and with known involvement in angiogenesis were strongly inhibited by su and less so by sti because platinumbased chemotherapy is part of the standard of care for sclc su was combined with cisplatin and significant tumor GD was measured compared with either agent CT these results expand the profile of su as a kit signaling inhibitor and suggest that su may have clinical potential in the treatment of sclc via direct antitumor activity mediated via kit as well as tumor angiogenesis via vascular endothelial growth factor receptor flkkdr and pdgfrbeta | MEDAL |
rising obesity incidence and its complications have lead to change of our view about cardiometabolic risk factors and need of reassessment of these complications in childhood age the aim of current T0 was to evaluate prevalence of obesity and related cardiometabolic risk factors of children this was a crosssectional T0 in a representative sample of years old children in rasht the biggest city in north of iran participants were interviewed and examined by a trained research team and demographic characteristics detailed examination height weight blood pressure were recorded blood samples were drawn for biochemical testing including fasting blood sugar triglyceride cholesterol hdl ldl data analysis was done using spss software total participants were children and were male prevalence of underweight NW overweight obese were respectively the prevalence of cardiometabolic risk factors included hypercholesterolemia HTG high ldl low hdl high systolic diastolic blood pressure percentile and abnormal carbohydrate metabolism revealed hypertriglyceridemia as the most common dyslipidemia correlation analysis didnt show significant correlation between bmi fbs but ldl cholesterol and tg had week positive correlation with bmi pearson rho respectively p value obesity and dyslipidemia are common problems in this area and we should note to screen cardiometabolic risk factors in addition of obesity especially in children with rapid weight gain this T0 highly recommended more investigation to evaluate final effect of these AF in adulthood | MEDAL |
a doubleblind randomized trial is described which was designed to compare the clinical effect of mg daily of MAP ludiomil and of clomipramine anafranil given by the intravenous route in forty hospitalized female patients with severe and resistant primary depression physicians assessment of patients progress was made following and days of treatment the response rates to maprotiline and clomipramine were not significantly different even if a greater number of patients responded to clomipramine none of the demographic and clinical variables considered could be identified as factor predicting the response to maprotiline and to clomipramine even if a trend for a preferential responsiveness to clomipramine in the older patients was obsl ligation and may be partially related to enhanced prostaglandin synthesis in the obstructed kidney on the other hand the higher adenylate cyclase activity and the change in campdependent protein kinase activity in glomeruli from the contralateral kidney may in part reflect increased pge production by these glomeruli the results support the concept that similar to their in vitro action locally generated prostaglandins may in vivo affect cortical tubular CF by influencing the tubular camp system while those generated in the glomerulus alter glomerular camp metabolism | MEDAL |
the distribution of GABA acidimmunoreactive gabai elements was examined in the septal region of the rat brain the indirect peroxidaseantiperoxidase technique was used with antigaba antibodies in normal and colchicinepretreated rats with or without use of detergent in the incubation medium intraventricular injection of colchicine did not result in any NC in the IF of neuronal perikarya intraseptal injections increased the intensity of labelling of gabai cell bodies in the lateral septal nucleus and increased the number of labelled cells in the medial septal nucleus and diagonal band of broca dbb triton x added to the incubation media decreased the intensity of staining and number of gabai somata in all septal nuclei with a concentrationdependent effect no change was observed concerning gabai varicosities the septal area including the lateral medial and triangular septal nuclei the anterior rudiment of the hippocampus the island of calleja magna the septofimbrial nucleus the bed nucleus of the ST and the dbb showed a strong reaction to antigaba antibodies with regard to gabacontaining surrounding structures gabai axonal varicosities were observed in all the regions with an uneven distribution the highest density was found in the dorsal and ventral parts of the lateral septal nucleus and in a band situated between the dbb and the nucleus accumbens labelled varicosities were frequently observed surrounding gabai and nonimmunoreactive cell HB gabai somata ranged from to micron in diameter small neurons were present in great number at the VVI border and in the zona limitans mediumsize and large SN were mostly observed in the medial part of the dorsal lateral nucleus and in the intermediate lateral nucleusabstract truncated at words | MEDAL |
we have studied the formation of histone hvdna complexes using an acoustic biosensor and afm imaging our results show that dna and histone molecules aggregate into amorphous accumulations which form a compact rigid layer on the sensors surface by measuring changes in the acoustic wave amplitude it was possible to titrate surface bound dna with hv and discriminate between dna molecules of different size and shape from the kinetic analysis of real time data keq was found equal to x m | MEDAL |
several publications in recent years have proven unbroken popularity of SF sural i.a. flaps for the coverage of defects of the lower leg and PT foot however complications are being discussed rarely the realistic complication rate is up to given corresponding comorbidities risk AF or performance PE twelve distally based neurovascular sural flaps were used for reconstruction of heel ankle and lowerleg defects between and the following pre and postoperative procedures were performed as a routine an additional arteriography was done preoperatively to prove the patency of the peroneal artery acoustic doppler ultrasound was used to identify the lesser RSV and the concomitant vessels perforators of the peroneal i.a. of the SN an enlarged skin paddle with a tail of skin over the pedicle at the point of rotation was harvested intraoperatively to take pressure off the vessels and facilitate primary closure postoperatively external fixation provides strict immobilization of the affected leg following these measures no flap loss was observed in the presented series and only few minor complications occurred | MEDAL |
a large abscess of the interatrial SP developed during the course of acute bacterial endocarditis affecting the aortic valve septic involvement of the cardiac conducting system produced atrioventricular dissociation and subsequent abscess rupture resulted in an aortoright atrial communication the clinical presentation of this rare complication of acute bacterial endocarditis is correlated with the postmortem findings | MEDAL |
characterization and metabolism of heparan sulfate glycosaminoglycans and proteoglycans hspgs synthesized by primary cultures of mouse uterine epithelial cells are reported hspgs were detected in both the medium and in the cellassociated fraction whereas glycosaminoglycans containing little or no protein free glycosaminoglycans were found primarily in the cellassociated fraction the cellassociated hspgs were relatively large kav on superose had a buoyant density in cesium chloride gradients of gml and contained heparan sulfate chains that fell into two size classes exhibiting kav values on superose of and respectively the free glycosaminoglycan chains displayed a kav on superose of the secreted hspgs were smaller median kav on superose of than the cellassociated hspgs more than of the cellassociated hspgs contained hydrophobic portions as evidenced by their ability to bind to octylsepharose in contrast only of the secreted hspgs bound to octylsepharose hspgs were detected at both apical and BCC surfacesextracellular matrices by indirect immunofluorescence in vitro and in utero and by accessibility to external PRs in vitro it was estimated that of the total cellassociated hspgs were exposed at the cell surface the hspgs released from the cell surface by proteases were slightly smaller than the IN hspgs and lacked the hydrophobic properties of the latter these observations suggested that the cell surface hspgs contain a small hydrophobic domain that functions in the att of hspgs to cells the free glycosaminoglycans appeared to be primarily intracellular and were not secreted the cellassociated hspgs turned over rapidly t h and appeared to be the precursors to the free glycosaminoglycans metabolic turnover of the free glycosaminoglycan pool was a relatively slow process t habstract truncated at words | MEDAL |
the heat stress transcription AF hsfs play a prominent role in thermotolerance and eliciting the HS response in plants ID and expression analysis of hsfs gene family members in chickpea would provide valuable information on HS responsive hsfs a genomewide analysis of hsfs gene family resulted in the identification of hsf genes in chickpea in both desi and kabuli genome phylogenetic analysis distinctly separated a b and c class hsfs respectively an analysis of cisregulatory elements in the upstream region of the genes identified many AS responsive elements such as HS elements hse abscisic acid responsive PE abre etc in silico expression analysis showed nine and three hsfs were also expressed in drought and salinity stresses respectively qpcr expression analysis of hsfs under heat stress at pod development and at days old seedling stage showed that carhsfa a and b were significantly upregulated in both the stages of crop growth and other four hsfs carhsfa aa ac ba showed early transcriptional upregulation for heat AS at seedling stage of chickpea these subclasses of hsfs identified in this T0 can be further evaluated as candidate genes in the characterization of heat stress response in chickpea | MEDAL |
the pleiotropic cytokine interleukin il has been demonstrated to increase during exercise little is known regarding the response of the soluble il receptors silr and sgp during such exercise the aim of the current T0 was to investigate the response of plasma il silr and sgp during fatiguing submaximal exercise in humans twelve participants underwent an incremental exercise test to exhaustion and one week later performed a submaximal exercise bout lactate threshold to volitional exhaustion blood samples taken at rest and immediately post exercise were analyzed for il silr and sgp il increased p by pg ml during the exercise period silr and sgp also increased p by ng ml and ng ml respectively the current study is the first investigation to demonstrate that alongside il acute exercise stress results in an increase in both silr and sgp | MEDAL |
a high incidence of transient neonatal hypothyroidism has been observed in premature infants T3 routine skin cleansing with iodine because these reports have been predominantly from europe a BL iodinedeficient area we wished to determine whether this was also true in north america an iodinesufficient area a prospective controlled study was performed in premature babies or weeks gestation admitted to a neonatal ICN thyroxine t and thyrotropin tsh were measured at day days to and to T3 skin preparation with iodine or with a noniodinecontaining antiseptic solution chlorhexidine that served as control if repeat cleansing was required this sequence was repeated urinary iodine was quantitated on days to to estimate iodine SE there was no difference in the mean t concentration at any of the time points evaluated nor in the incidence of transient hypothyroidism between the iodineexposed and control babies despite UI excretion up to times the control value unexpectedly iodineexposed but control babies developed severe hypothyroxinemia t nmoll compatible with the sick euthyroid syndrome one of them died we conclude that unlike in europe transient HT is not a common sequela of routine skin cleansing with iodine in premature P0 infants in north america this difference in incidence may be due to prior iodine status whether excessive iodine absorption in premature infants is associated with thyroidindependent toxic effects remains to be clarified | MEDAL |
a simple method using solidphase extraction combined with metal furnace AAS was developed for the determination of criii and crvi at subppb C2 in water a ml water sample was adjusted to ph with nitric acid and then passed through an iminodiacetate extraction disk placed on a cationexchange extraction disk at a flow rate of ml min for concentrating criii the filtrate was adjusted to ph with AQ ammonia and then passed through an anionexchange SE disk at a flow rate of ml min for concentrating crvi the criii and crvi collected were eluted with ml of mol l nitric acid for criii and ml of g l diphenylcarbazide solution for crvi each eluate was diluted to ml with DW and injected into a utype tungsten board on the metal furnace the calibration curves of criii and crvi showed good linearity in the range of ng the LOD corresponding to three times the sigma n of blank values were pg for both criii and crvi the analytical value of total cr criii crvi in certified REF material of river water jsac was in good agreement with the REF value the REC test for µg µg l of criii and crvi added to ml of the water samples showed sufficient values except for river water sampled downstream due to relatively higher codmn value the relative standard deviations n were less than for both criii and crvi | MEDAL |
midazolam is gradually replacing diazepam in neuroleptanalgesia with FE and sufentanil because of its greater water solubility greater hypnotic potency shorter halflife lack of pharmacologically active metabolites and low incidence of thrombophlebitis in order to substantiate midazolam as being as safe and ERP as diazepam in cardiac patients with severe coronary occlusion hemodynamic measurements were made before and min T3 completion of i.v. of mgkg iv midazolam n and mgkg iv diazepam n the doses which are MZ to those used in patients undergoing cardiac catheterization all patients were spontaneously breathing o in air no positional changes orum st height significantly higher than the control group standard error vs microv p unlike the control subjects maximal arm ergometry exercise in the high level injury subjects failed to decrease stsegment height delta st vs microv in controls p this difference persisted even T3 matching for exercise capacity however during exogenous stimulation with the sympathomimetic amine isoproterenol stsegment height in the high level injury group markedly decreased mean delta st vs microv in controls p thus CE sympathetic dysfunction regularly results in multilead ST that decreases to or below isoelectric baseline during low dose isoproterenol infusion unlike normal subjects and individuals with normal variant stsegment elevation st height is not altered by exercise these findings document that stsegment height in man is greatly influenced by CE sympathetic nervous activity both at baseline and during physiologic and pharmacologic AS | MEDAL |
as a consequence of improved SQ of abdominal imaging techniques in the last decades discovery of pancreatic cystic lesions has become more common the clinical significance of these lesions is often unclear and poses a diagnostic dilemma endoscopic ultrasoundguided fineneedle aspiration eusfna is a subject of debate regarding its role in the diagnostic DUE of pancreatic masses and cysts although risks associated with the procedure are low consequences can be serious and even lifethreatening we report a case of a previously healthy yearold woman who suffered severe acute pancreatitis after eusfna of a pancreatic cyst requiring admission to the intensive ECU icu OD of infected pancreatic necrosis and successively bowel ischaemia led to MOF despite maximal antibiotic and surgical treatment the patient succumbed to refractory septic shock the fatal outcome of this case illustrates the importance of balanced decisionmaking in the diagnostic RPA of pancreatic cystic lesions | MEDAL |
plasmodium falciparum pf malaria causes million cases worldwide million being severe and complicated leading to â¼ million deaths and â¼ abortions annually plasmodium falciparum erythrocyte membrane protein pfemp has been implicated in cytoadherence and infected erythrocyte rosette formation associated with CM chondroitin sulphatea att and INF erythrocyte sequestration related to PAM and other severe forms of disease an EC cell high activity IB peptide is described in several of this â¼ kda HV proteins domains displaying a conserved motif gacxpxrrxxlc it established hbonds with other binding peptides to mediate red blood cell group a and CS attachment this motif when properly modified induced pfempspecific straintranscending fullyprotective immunity for the first time in experimental challenge in aotus monkeys opening the way forward for a long soughtafter vaccine against severe malaria | MEDAL |
a polydimethylsiloxane stamp was applied for the first time to the fabrication of nchannel thinfilm TFTs based on soluble small molecule organic semiconducting materials the stamping method was found to facilitate film transfer onto a gate insulator surface irrespective of its surface free energy we used phenylcbutyric acid methyl ester pcbm and cfused nmethylpyrrolidinemetadodecyl phenyl cmc as nchannel materials the stamped thinfilm TFTs of cmc achieved a high electron mobility of cmv s and a current onoff ratio of à the mobility of the stamped cmc thinfilm TFTs did not depend much on the surface free energy of the sio gate insulator with and without surface treatment using a silanecoupling reagent in particular the stamped cmc thinfilm transistor exhibited a relatively high mobility of cmv s on a high SE surface of UT sio in addition a cDNA inverter composed of an nchannel and a pchannel stamped thinfilm transistor was demonstrated for the first time which exhibits a maximum gain of at a supply Vc of v | MEDAL |
the hormone calcitonin ct is primarily known for its pharmacologic action as an inhibitor of BR yet ctdeficient mice display increased bone formation these findings raised the question about the underlying cellular and molecular mechanism of ct action here we show that either ubiquitous or osteoclastspecific inactivation of the murine ct receptor ctr causes increased bone formation ct negatively regulates the OCL expression of spns gene which encodes a transporter for the signalling lipid sphingosine phosphate sp ctrdeficient mice show increased sp C2 and their skeletal phenotype is normalized by deletion of the sp receptor sp finally pharmacologic treatment with the nonselective sp IL-1ra fty causes increased bone formation in wildtype but not in spdeficient mice this study redefines the role of ct in skeletal biology confirms that sp acts as an osteoanabolic molecule in vivo and provides evidence for a pharmacologically exploitable crosstalk between osteoclasts and OBs | MEDAL |
diabetic rats were used to test a previous hypothesis that alterations in VL hypothalamic vlh fatty acid oxidation observed in over and underfed rats were a function of the animals peripheral energy balance and not merely a function of their SE NI standard adaptations to the diabetic condition were exhibited in streptozotocin diabetic rats such as depressed body weights hyperphagia and hyperglycemia elevated SS free fatty acids depressed insulin concentrations depressed hepatic glucose oxidation and elevated hepatic HFA oxidation rates of vlh FAO to co and to an acid watersoluble fraction in diabetic rats were elevated relative to nondiabetic rats the alterations in vlh fatty acid oxidation in diabetic rats were similar to changes previously observed in animals exhibiting a NEB the results were discussed with respect to the concept that vlh FAO was a component in the recognition of IP energy balance and in part served to alter the regulators of EB and food intake | MEDAL |
in the present study we have attempted to characterize in mice a situation which appears to simulate real life predation and elicits simultaneous analgesia and immobility we utilized pinch produced by clip application to various regions of the body and examined its effect on responsiveness to noxious stimuli and motor behavior intense noxious clip was applied to the nape of the neck back and base of the tail the area most effective for the elicitation of both clip induced analgesia and immobility was the nape of the neck while tail pinch resulted in analgesia but not immobility evidence is provided that different systems are responsible for clip induced immobility and analgesia temporal dissociation of clip induced analgesia and immobility could be demonstrated with continuous clip application for min showing a different time course for the analgesic and immobilizing effects different stimuli were effective in eliciting clip induced analgesia and immobility with noxious stimuli essential for the induction of clip induced analgesia and innocuous stimuli sufficient for clip induced immobility thus low analgesic doses of LAs injected into the nape of the neck prevented noxious clip from inducing analgesia but immobility was still evident in contrast nonnoxious pinch to the nape of the neck elicited immobility but not analgesia and clip induced immobility could still be induced after the administration of high doses of morphine which completely blocked responses to noxious stimuli these results demonstrate that in a situation resembling NK predation both analgesia and immobility are produced concurrently but that these behavioral phenomena can be elicited differentially and may be mediated by different independent systems | MEDAL |
deficiencies of AT at protein c pc or protein s ps and APCR apcr are very wellestablished coagulation defects predisposing to A-V thromboembolism vte we performed a retrospective cohort family study to assess the risk for vte in individuals with at pc or ps deficiency or apcr five hundred thirteen relatives from italian centers were selected from families in which the proband had had at least episode of vte we calculated the incidence of vte in the whole cohort and in the subgroups after stratification by age sex and defect the overall incidence of vte per patientyears in the group of relatives was it waied to the land as an organic fertilizer knowledge of nutrient content of soils and crop uptake of nutrients is incorporated into manure application and crop removal plans to prevent either runoff or nutrient buildup on the land this is to ensure water SQ protection existing systems are adequate but lack flexibility require ERP management may not have been incorporated into older farms and do not offer obvious solutions to odor concerns costeffective alternatives should address those needs advancement in nutrient management procedures will likely accelerate the ongoing changes in the structure of the swine industry | MEDAL |
a yearold man attempted suicide by burning charcoal in a car for more than one day and was admitted to a regional hospital on admission his blood carboxyhemoglobin concentration was the patient was transferred to our emergency department because of suspected carbon monoxide poisoning hyperbaric oxygen therapy hbo was performed times over days fluidattenuation inversion REC flair and diffusionweighted dwi magnetic resonance imaging mri performed on day showed high signalintensity lesions in the cerebral VWM additional hbo was performed once per day until day wecheler SM scalereviced wmsr and minimental state examination mmse performed on day showed his cognitive impairment he gradually recovered the cognitive CF and was discharged from the hospital without neurological sequelae on day delayed HIVE T3 acute carbon monoxide poisoning with dementia mental impairment and psychosis is a serious complication hyperintensity in flair and dwi mri predicts delayed encephalopathy and indicates cellular edema and demyelination of the WG matter one of the risk factors is prolonged carbon monoxide exposure this case suggests that the patient who was exposed to carbon monoxide for many hours was at a high risk of delayed HIVE despite the low blood carboxyhemoglobin concentration and therefore must be monitored using mri | MEDAL |
the OD of an enzyme based biosensing method is described for evaluating the toxicity of solutions treated by titanium dioxide photocatalysis the method is based on the potential of rat liver microsomal glutathione transferase ability mgst to get enhanced in the conditions of chemical and oxidative toxicity phenol is taken as a MM pollutant due to its toxicity and prevalence in industrial processes chemical analysis of the parent compound products and LD50 assays using the mgst activity were conducted during and T3 the various photocatalytic treatments the maximum mgst activity was observed from and min treated samples this PT toxicity might be due to toxic phenolic products which may include pbenzoquinone hydroquinone benzenetriol and other intermediates the enzymatic activity pattern observed after photocatalytic treatment corresponded well with the chemical degradation data obtained by hplcuv the mgst assay seems to be an easy to use and promising RPA for evaluating the effectiveness of wastewater treatment processes | MEDAL |
two female patients with ebsteins anomaly aged and years underwent new procedures of tricuspid valve replacement in the true annulus without involvement of atrioventricular conduction disturbance the first patient had a relatively small atrialized right ventricle a dacron patch was sutured on the atrialized portion from the right ventricular side buttress stitches were placed on the whole of the atrialized IVT except at the part of the his bundle a porcine valve was sutured in the true annulus of the TV and on the top of the patch the second patient had a fairly large atrialized IVT and plication was made only in the inferior part of the atrialized ventricle form the coronary sinus a porcine valve was sutured in the true tricuspid annulus except at the part of kochs triangle where the porcine valve was placed on the false annulus | MEDAL |
the effect of lowpressure plasma on quality attributes of meat is an important aspect which must be considered before application in food the aim of this T0 was to determine the color HFA composition lipid oxidation expressed as thiobarbituric acid reactive substances and total antioxidant capacity of raw pork samples exposed to helium LP plasma treatment kpa for and min during the storage period the thiobarbituric acid reactive substance concentrations of all plasmatreated samples during storage were in the range from to mg malondialdehydekg exposure time caused significant changes only in total color difference hue angle and chroma T3 min of treatment ferric reducing ability of plasma values of meat samples decreased from to mmol trolox eqkg T3 days of storage the storage period significantly affected proportion of polyunsaturated fatty acids with an increase about after days of refrigeration storage while the content of saturated fatty acids was at the same level helium LP plasma does not induce oxidative processes application of this decontamination technique while maintaining product SQ is possible in food industry | MEDAL |
fracture of porcelain is a relatively common clinical misfortune recent research has indicated that strong bonds can be formed between composite and dental porcelain porcelain surfaces are etched with hydrofluoric acid and treated with silane coupling agents before composite application the question is how exposed dentin may react to etching with hydrofluoric acid | MEDAL |
a consecutive series of fresh fractures of the tibial shaft was internally fixed by the new dynamic compression SP asifdcp all but cases could be reviewed personally within years postoperatively while patients had died the PET results and postoperative complications are reported separately for the closed and open fractures inspite of a higher rate of complications in the open fractures their functional results were about as good as in the closed cases in our experience the new dynamic compression plate proved to be very satisfactory for internal fixation of fractures of the tibia | MEDAL |
we analysed AT concentrations of persistent organic pollutants pops in cases with exocrine pancreatic CA the comparison group consisted of subjects significantly increased concentrations of polychlorinated biphenyls pcbs hexachlorobenzene hcb sum of CHLs and polybrominated diphenylethers pbdes were found in the cases for dichlorobispchlorophenylethylene ppdde no significant difference was seen for pcbs no odds ratio or could be calculated since all cases had concentrationmedian in controls used as a cutoff hcb yielded or confidence interval ci and sum of chlordanes or ci whereas or was not significantly increased for ppdde or pbdes body mass index bmi at the time of tissue sampling was significantly lower for the cases this might have influenced the results using bmi one year previously or decreasing the concentrations of pops with the same percentage as weight loss among the cases did not NC the results survival of the cases was shorter in the group with the concentration of popsmedian among cases significantly so for the sum of pcbs vs days ppdde vs days and sum of chlordanes vs days in the high and low group respectively the results were based on a low number of cases and should be interpreted with caution | MEDAL |
clomethiazole cmz was used as a model drug to be incorporated into an emulsion vehicle the effects of drug concentration and number of homogenisation steps were evaluated using MLR the droplet size measured as a zaverage diameter by photon correlation spectroscopy pcs was found to be between and nm in the investigated range of cmz concentrations highly dependent on the concentration but more weakly so on the number of homogenisation steps slowscanning highsensitivity differential scanning calorimetry dsc measurements showed that cmz depresses the phospholipid chain melting temperature in the emulsion system whereas c nuclear magnetic resonance nmr experiments suggested that the cmz molecules are to a large extent located in the surface region of the w/o/w droplets this interpretation is compatible with results from nmr selfdiffusion measurements which showed that most of the cmz molecules are rapidly exchanged between emulsion droplets and the AQ surrounding it can be concluded that the surfaceactive drug cmz has a significant influence on the characteristics of phospholipidstabilised emulsions through its ability to interact with the phospholipid interface thus the results underline the importance of characterising druglipid interactions for the OD of lipidbased formulations | MEDAL |
we encountered a case of hiv infection in a previously healthy man which was characterized by rapid progression to aids and death within months in association with high levels of antigenemia throughout the clinical course and no humoral immune response for at least months genetic changes of the third variable domain v of the envelope gene of hiv in serum samples were analyzed at four time points during his rapid clinical course the nucleotide changes were confined to a maximum of three substitutions among nucleotides of the v region a major population of the viral clones in this patient showed one CAA substitution from aspartic acid a negatively charged amino acid to lysine a positively charged CAA at position from the first cysteine of the v loop this substitution was thought to be associated with phenotypic changes and viruses with this sequence in the v region had a strong syncytiuminducing ability in mt cells it appears that the lack of a humoral immune response accelerated PD in our patient and a genetic NC that appeared to produce a phenotypic NC occurred at an early stage of the disease | MEDAL |
human leukocyte elastase hle interacts with hiv glycoprotein gp suggesting a nonenzymatic receptor function for hle in the context of hiv hle is found localized to the cell surface but not granules in hiv permissive clones and to LG but not the cell surface of hiv nonpermissive clones inducing cellsurface hle expression on hle null hiv nonpermissive clones permits hiv infectivity hiv IB and infectivity diminish in proportion to hle rna subtraction hiv binding and infectivity show dose dependence for the NK hle ligand alpha proteinase inhibitor alphaantitrypsin alphapi chemokines prevent whereas alphapi promotes copatching of hle with the canonical hiv receptors recent demonstration that decreased viral rna is significantly correlated with decreased circulating alphapi in hiv SP individuals is consistent with a model in which hle and alphapi can serve as hiv coreceptor and cofactor respectively and potentially participate in the pathophysiology of hiv PD | MEDAL |
nineteen subjects with isolated EIA fev fef pefr fvc greater than predicted values fall in fev of at least T3 exercise typical recent PS of exerciseinduced asthma no other treatment were entered in a multicenter trial carried out in a double blind double PL crossover manner after a onemonth baseline period subjects underwent an exercise after inhaling micrograms of SM n or mg of sodium cromoglycate n treatments were alternated before the second exercise which took place at least days T3 the first tau was assessed by examining changes in fev fef after exercise carried out minutes and hours after administering the treatment by comparison with baseline values assessments done and minutes after exercise lowest of three values kept for the analysis of each parameter fev and fef were significantly higher T2 after taking SM salmeterol was found to be superior to sodium cromoglycate for all parameters hours T3 administering the drug both treatments were well tolerated this T0 confirms that the longer duration of effect of salmeterol and its superiority by comparison with the standard treatments of exerciseinduced asthma | MEDAL |
multicenter trials have demonstrated that in patients with sinus rhythm ivabradine is effective in the therapy of ICM HR disease and of impaired LVSF ivabradine is ineffective in atrial fibrillation many patients with symptomatic HR failure have DD with preserved LVSF and many have asymptomatic paroxysmal atrial fibrillation ivabradine is not indicated in these conditions but it happens that it is erroneously used digoxin is now considered an outdated and potentially dangerous drug and while ERP in the mentioned conditions is rarely used the aim of the study was to compare the therapeutic effects of ivabradine in diastolic heart failure with preserved LVSF patients were assigned to ivabradine or digoxin according to a randomization crossover design data were singleblind analyzed the analysis was performed using an ITT method fortytwo coronary patients were selected in spite of maximally tolerated therapy with reninantagonists diuretics and βblockers they had congestive diastolic heart failure with preserved systolic CF both ivabradine and digoxin had positive effects on dyspnea nterminal natriuretic peptide HR rate duration of min walktest and signs of DD but digoxin was highstatistically more effective sideeffects were irrelevant data were obtained in a singlecenter and from patients with ICM etiology of HR failure the number of patients is small and does not allow assessing mortality in coronary patients with symptomatic diastolic HF with preserved systolic function lowdose digoxin was significantly more effective than ivabradine and is much cheaper one should be more critical about ivabradine and lowdose digoxin in DHF to avoid possible negative effects on the cardiac CF and a severe reduction of the cardiac SO the resting heart rate should not be decreased to bpm | MEDAL |
highdose methotrexate hdmtx added to a basic regimen of chemotherapy proved superior to cranial irradiation and sequentially po drug pairs rtsc in prolonging complete remissions in children with standardrisk ALL leukemia to extend this result to more contemporary risk groups we reclassified the patients according to methods of the pediatric oncology group pog the childrens cancer T0 group ccg the rome workshop and st jude total therapy study xi by life table analysis to of patients with a favorable prognosis would remain in CCR ccr at years if treated with hdmtx uniformly lower ccr rates could be expected with rtsc especially in st jude betterrisk patients hdmtx also would show greater tau than rtsc in the ccg averagerisk and pog poorrisk CG but the results appear VPI to those being achieved with intensified regimens for highrisk leukemia although both therapies would provide adequate cns prophylaxis in favorablerisk CG rtsc would offer greater protection in patients classified as being in a worserisk group by st jude criteria we conclude that hdmtxbased therapy as described in this report would be most ERP in patients with a presenting leukocyte count of less than x l of the white race aged to years and having leukemic cell hyperdiploidy without translocations | MEDAL |
gradients of the fast transient outward k current itof contribute to heterogeneity of VVI repolarization in a number of species cardiac itof C2 and gradients change notably with HR disease human cardiac itof appears to be encoded by the kv poreforming αsubunit plus the auxiliary kchip βsubunit while mouse cardiac itof requires kv and kv αsubunits plus kchip regional differences in cardiac itof are associated with expression differences in kv and kchip although itof was reported to be absent in mouse VVI cardiomyocytes lacking the kv gene kv when short depolarizing voltage pulses were used to activate voltagegated k currents in the present T0 we showed that the use of long depolarization steps revealed a heteropodatoxinsensitive itof at of the wildtype C2 immunohistological studies further demonstrated membrane expression of kv in kv cardiomyocytes transmural itof gradients across the left ventricular wall were reduced by fold in kv heart compared to wildtype the itof gradient in kv NF was associated with gradients in kchip mrna expression while in wildtype there was also a gradient in kv expression in conclusion we found that kvbased itof exists in the absence of kv although with a reduced transmural gradient kv mice may be a useful rat for studying kvbased itof as observed in humans | MEDAL |
the pygmy hog is a representative of the smallest and rarest wild species of known living suidae this paper reports the investigation of haemorrhagic enteritis encountered amongst the pygmy hogs at the research and breeding centre of the pygmy hog conservation programme guwahati assam india three out of pygmy hogs died of enteric infection postmortem examination and bacteriological investigation of two out of the three animals that died revealed clostridial infection the isolates harboured two plasmids of molecular weight kilobases kb and kb clostridium perfringens type a positive for the beta toxin cpb gene was detected by PCR reaction sequence analysis of the partial alpha toxin cpa gene showed to homology with isolates from different geographical locations | MEDAL |
rabbits were immunized with bTSH btsh bovine inteinizing hormone blh and their subunits in two immunization experiments thyroidstimulating activity was found in the serum of out of rabbits immunized with btshbeta subunits the TSA in the antibtshbeta sera was greater at h than at was eluted with the AHG fraction from sephadex g was completely neutralized by both antibtsh and antirabbit gamma AHG and was CR suppressed by administration of triiodothyronine t to the immunized rb these findings led to the conclusion that the thyroidstimulating activity resided in soluble complexes of rabbit tsh bound to antibtshbeta two of nine rabbits immunized with btsh developed TSA in their serum but it was nonsuppressible by t none of the animals immunized with btshalpha blh blhbeta or blhalpha developed serum thyroidstimulating activityhypopituitary HT evidenced by decreased serum thyroxine t and thyroidal i uptake and by the histologic appearance of C1 follicles with flat cells was found in the btshbeta and btshimmunized animals despite the presence of TSA in the serum of many the reasons for this paradox are unclear possibly the complexes block the effect of tsh on the rb thyroid | MEDAL |
metastatic malignant melanoma is an extremely aggressive cancer with no currently viable therapy allylmethoxyphenol eugenol was tested for its ability to inhibit proliferation of melanoma cells eugenol but not its isomer isoeugenol methoxypropenylphenol was found to be a potent inhibitor of melanoma cell proliferation in a b xenograft study eugenol treatment produced a significant RT growth delay p an almost decrease in tumor size and a increase in the median time to end point more significantly of the animals in the control group died from metastatic growth whereas none in the treatment group showed any signs of invasion or metastasis eugenol was well tolerated as determined by measurement of bodyweights examination of the mechanism of the antiproliferative action of eugenol in the human malignant melanoma cell line wmlu showed that it arrests cells in the s phase of the Tc FC coupled with biochemical analyses demonstrated that eugenol induced apoptosis cdna array analysis showed that eugenol caused deregulation of the ef family of transcription factors transient transfection assays and electrophoretic mobility shift assays showed that eugenol inhibits the transcriptional activity of ef overexpression of ef restored about of proliferation ability in cultures these results indicate that deregulation of ef may be a key factor in eugenolmediated melanoma growth inhibition both in vitro and in vivo since the ef transcription factors provide growth impetus for the continuous proliferation of melanoma cells these results suggest that eugenol could be developed as an eftargeted agent for melanoma treatment | MEDAL |
during breast CA OD increased presence of leukocytes in neoplastic stroma parallels disease progression however the PET significance of leukocytes in regulating protumor versus antitumor immunity in the breast remains poorly understood utilizing the mmtvpymt MM of mammary carcinogenesis we demonstrate that ilexpressing cd t lymphocytes indirectly promote invasion and subsequent metastasis of mammary adenocarcinomas by directly regulating the phenotype and effector CF of tumorassociated cdbgrf macrophages that in turn enhance metastasis through activation of epidermal growth factor receptor signaling in malignant mammary epithelial cells together these data indicate that antitumor acquired immune programs can be usurped in protumor microenvironments and instead promote malignancy by engaging cellular components of the innate immune system functionally involved in regulating epithelial cell SMB | MEDAL |
through molecular dynamics simulations considering thermal vibration of surface atoms ionic behaviors in concentrated nacl solutions confined between discretely charged Si surfaces have been investigated the EDL structure was found to be CS to the density and CSD of surface charges due to the discreteness of the surface charge a slight charge inversion appeared which depended on the surface charge density bulk concentration and confinement in the nanoconfined nacl solutions concentrated from to m the locations of accumulation layers for na and cl ions remained stable but their peak values increased the higher the concentration was the more obvious the charge inversion appeared in m nacl solution na and cl ions show obvious AC layered distributions which may correspond to the solidification found in experiments by changing surface separation the confinement had a large effect on the ionic CSD as both surfaces approached each other many ions and water molecules were squeezed out of the confined space two adjacent layers in ion or water distribution profiles can be forced closer to each other and merge together from ionic hydration analysis the coordination number of na ions in highly confined space was much lower than that in the bulk | MEDAL |
a case of nine dermatofibromata including a giant one associated with necrobiosis lipoidica and diabetes mellitus type ii is reported this case is unusual because of the number and size of the PT and their association with the abovementioned pathologic conditions | MEDAL |
polycythaemia in the neonate is a serious pathologic entity which occurs particularly in infants of diabetic mothers idm and smallforgestational age sga infants both of these conditions are associated with fetal hyperinsulinaemia cultures of cord blood MNC cells from polycythaemic idm showed increased growth of late erythroid progenitor colonies compared to cord blood MNC cells from nonpolycythaemic infants reflecting a possible expansion of this progenitor population in the polycythaemic fetus no changes were observed in early erythroid progenitor populations biosynthetic human insulin at physiological C2 characteristic of idm stimulated growth in culture of late erythroid progenitors in cord blood from premature term and idm infants three out of five polycythaemic infants had elevated cord BP C2 of insulin cpeptide at birth whereas no infant with a PCV of less than had high insulin cpeptide measurements these data suggest that the polycythaemia noted in infants of diabetic mothers may be AA in C1 part to a stimulatory effect on erythroid progenitor growth by the hyperinsulinaemic environment in which they develop in utero | MEDAL |
in the past the method of reconstitution was used to investigate the interaction between metalloenzymes containing znii and metal ions in this paper electron paramagnetic resonance epr has been employed to firstly study the direct interactions between bacillus subtilis neutral proteinase bsnp nuclease p and cuii ions added in aqueous solution respectively these results show that a dynamic equilibrium exists between the znii in the AS site of native enzymes and the added cuii the added cuii partly replaces the znii forming cuiienzyme derivatives as a result the activity of the native enzymes is influenced the influences of ph value on this kind of interaction have also been investigated and the results demonstrate that the NC of ph value has little influence on the system of nuclease p but has remarkable influence on bsnp we firstly obtained the epr spectra for cuiienzyme derivatives in addition the derivative of cuiibsnp exists in the solution with two different conformations i type gparallel aparallel mt ii type gparallel aparallel mt and this two conformations exchanged each other depending on ph | MEDAL |
aneurysmal bone cysts are rare benign lesions of bone tissue infrequent in craneofacial skeleton with regard to other structures like long bones or the spine they are composed of sinusoidal and V1 spaces bloodfilled and surrounded by fibrous tissue septa we present a case of a yearold caucasian male with a big swelling in the left mandible associated to pain and rapid growth he referred previous extraction of the left inferior third molar on the xray study an expansive multilocular and high vascularized bony lesion within the mandibular angle was observed it produced expansion and destruction of lingual and buccal SC an incisional biopsy was performed showing a fibrous tissue with bloodfilled spaces lesion suggestive of an aneurysmal bone cyst after selective embolization of the tumour surgical resection was done with curettage and immediate reconstruction of the defect with an anterior IC graft aneurysmal bone cysts are nonneoplastic but locally aggressive tumours with occasional rapid growth that may be differentiated from other multilocular process like ameloblastoma ossifying fibroma epithelial cyst giant cell granuloma and sarcomas treatment of choice consists on conservative surgical excision of the mass with curettage or enucleation when resection creates a big defect primary surgical reconstruction is recommended | MEDAL |
recent multivariate analyses of fmri activation have shown that discriminative classifiers such as support vector machines svm are capable of decoding fmrisensed neural states associated with the visual presentation of categories of various objects however the lack of a generative model of neural activity limits the generality of these discriminative classifiers for understanding the underlying neural representation in this study we propose a generative classifier that models the hidden AF that underpin the neural representation of objects using a multivariate multiple linear regression model the results indicate that object features derived from an independent behavioral feature norming study can explain a significant portion of the systematic variance in the neural activity observed in an objectcontemplation task furthermore the resulting regression MM is useful for classifying a previously unseen neural activation vector indicating that the distributed DP of neural activities encodes sufficient signal to discriminate differences among stimuli more importantly there appears to be a double dissociation between the two classifier approaches and within versus betweenparticipants generalization whereas an svmbased discriminative classifier achieves the best classification accuracy in withinparticipants analysis the generative classifier outperforms an svmbased MM which does not utilize such intermediate representations in betweenparticipants analysis this DP of results suggests the svmbased classifier may be picking up some idiosyncratic patterns that do not generalize well across participants and that good generalization across participants may require broad largescale patterns that are used in our set of intermediate semantic features finally this intermediate representation allows us to extrapolate the MM of the neural activity to previously unseen words which cannot be done with a discriminative classifier | MEDAL |
male exp rats gm liveweight were distributed into groups of animals each and received balanced diets with the exception of lysine which was added to the diets in graded amounts in such a way that the lysine content of the diets ranged from to gm gm n T3 a feeding period of days the animals received h and c lysine injected intraperitoneally animals of each group were investigated for the total co excretion and co excretion during the firristic to enable the diagnosis to be reached in advance of decreased spinal mobility immobility pain and stiffness and bilateral SI pain are the most characteristic features but additional support is provided by the presence of any of the following onset by age a raised sedimentation rate peripheral joint involvement circumthoracic pain family history and iritis | MEDAL |
transformation of chicken cells by rous sarcoma virus rsv requires the functional expression of the viral src protein a IMT protein kinase ppsrc SCV of rsv containing deletions within the amino terminal onethird of the src protein have been identified that exhibit either temperaturesensitive or transformationdefective phenotype when used to infect CEC to define the regions within the amino terminal portion of ppsrc that influence morphological transformation a series of overlapping deletion mutations in the src gene of prague a rsv pr a rsv were constructed and their biological and biochemical properties were analyzed deletions within the src gene which remove CAA residues to had minimal effects on the ability of the mutant viruses to induce cellular transformation however deletions which impinged upon the region of the src gene encoding residues to inhibited cellular transformation a variant containing a deletion of amino acid residues to was temperature sensitive for transformation structurally altered src proteins recovered from cells infected with td variants exhibited a somewhat reduced IMT protein kinase MICs when assayed in the immune complex kinase CA analysis of the in vivo phosphorylation of a ppsrc substrate the kda protein revealed virtually wildtype C2 of phosphorylation in cells infected with the transformationdefective mutants these studies suggest that the region of the pr a rsv src protein delineated by amino acid residues to is essential for initiation and maintenance of MT of chicken cells in culture | MEDAL |
structural correlations between allergic symptoms and betalactam antibiotics were investigated by the use of leucocyte migration inhibition tests on patients in whom allergy to the drugs was detected from among patients suspected of having betalactam hypersensitivity no significant difference was found in the allergic PS between the mother nucleus structure of betalactam antibiotics or the c side chain structure of cephem antibiotics but in both betalactam and cephem antibiotics those with an aminothiazolyl group in the acylamido in their side chain structure induced fever or hepatopathy significantly more often than skin eruption in contrast drugs with a benzyl group induced skin eruptions significantly more often than hepatopathy leucocyte migration SAF lmaf was found significantly more often than leucocyte GABA factor in patients with fever or hepatopathy and cephem antibiotics with an aminothiazolyl group in the acylamido side chain produced lmaf at a very high rate our findings indicate that the specificity of the chemical structure of betalactam antibiotics and allergic symptoms is dependent on the acylamido side chain structure and an aminothiazolyl group structure has a high capability of inducing fever or hepatopathy moreover the high lmafinducing ability of the aminothiazolyl group structure is involved in this pathogenic mechanism | MEDAL |
we report an albino B6 mouse CS carrying a spontaneous mutation in the tyr cblntyrcwtsi deep whole genome sequencing of founder mice revealed very little divergence from cblnj and cbln taconic this coisogenic strain will be of great utility for the international mouse phenotyping consortium impc which uses the eucommkomp targeted cbln es cell resource and other investigators wishing to work on a defined B6 background | MEDAL |
the e alpha mhc class ii gene with kb of flanking and kb of flanking sequences was introduced into hb x sf mice which do not express their endogenous e alpha gene the Tg was expressed in thymic tissue and in adherent spleen cells and was induced in peritoneal exudate cells by gammainterferon in contrast to the normal e alpha gene there was no expression in b lymphocytes since transgenic animals made with constructs containing kb and kb of flanking sequences show normal expression pattern of the e alpha gene it appears that deletion of flanking sequences between kb and kb inactivated or eliminated regulatory sequences required for expression of e alpha specifically in b cells the presence of pbr dna linked to the kb e alpha transgene suppresses expression in IP adherent cells yielding mice expressing e alpha only in the thymus these mice appear to be tolerant to ie as measured in mixed leukocyte response experiments | MEDAL |
there are two approaches to care during the L3 of labour AS management includes three components administration of a prophylactic uterotonic drug cord clamping and controlled cord traction for physiological care intervention occurs only if there is clinical need evidence to guide care during the third stage is limited and there is variation in recommendations which may contribute to differences in practice this paper describes current uk practice during the third stage of labour | MEDAL |
while it may be a long time before we can specify the mechanisms through which a brain process achieves awareness it may be possible to determine as a first step whether awareness is limited to the products of certain kinds of processing in the domain of vision for example perceptual awareness might only be attainable in association with objectcentred coding configural representations of space and other such forms of abstracted nonretinocentric coding it appears that these forms of visual coding are anatomically restricted to telencephalic structures and indeed it has been argued that they may be peculiar to or at least visually dependent upon the VP stream of visual areas with the SC it is suggested here that such a brain process would still not be able to enter visual awareness unless it was selectively amplified through neuronal gating of the kind that has been shown to be correlated with selective spatial attention the present paper explores the extent to which this putative dual requirement for visual consciousness might form a basis for understanding the various phenomena of covert vision seen in patients suffering from hemianopia apperceptive agnosia and unilateral spatial neglect | MEDAL |
fabry disease is an xlinked recessive disorder caused by a deficiency of the lysosomal hydrolase alphagalactosidase a alphagal the deficiency of this enzyme leads to the systemic deposition of ceramide trihexoside cth in various tissues and organs enzyme replacement using iv doses of recombinant human alphagal produced in cho cells or in human fibroblasts is currently being evaluated in clinical trials as a potential therapy for this disease however it requires lifelong therapy involving a C1 amount of purified alphagal as a novel RPA for treatment of fabry disease we used polymer encapsulated chinese hamster ovary cho cells GM to express alphagal the secreted high levels of alphagal passed through the semipermeable polymeric membrane using coculture system with fabry fibroblasts the secreted enzyme was taken up in cells resulting in reduced accumulation of cth in fabry fibroblasts this in vitro study demonstrated that an encapsulated alphagalsecreting cell L1 can be used to treat fabry mice by transplantation in vivo judging from the protection against immune AAR by a semipermeable synthetic membrane this novel RPA may be applied to treat patients with fabry disease and other lysosomal storage diseases | MEDAL |
in this crosssectional T0 associations were examined between eight shift schedule characteristics with shiftspecific sleep complaints and need for recovery and generic health and performance measures it was hypothesized that shift schedule characteristics meeting ergonomic recommendations are associated with better sleep need for REC health and performance questionnaire data were collected from shift workers of companies with regular semicontinuous shift schedules the shift schedule characteristics were analyzed separately and combined using multilevel LR models the hypothesis was largely not confirmed relatively few associations were found of which the majority was in the direction as expected in particular early starts of morning shifts and many consecutive shifts seem to be avoided the healthy worker effect limited R2 between included schedules and the crosssectional design might explain the paucity of significant results | MEDAL |
this study examined the burden of zn cu ni pb cr and v in patches of asphyxiated mangrove swamp areas within qua iboe estuary mangrove ecosystem by sediments and surface water analysis in order to establish NK BG levels of these metals and to assess anthropogenic influences on them the analysis shows that the mean concentrations mgkg dw ofence of EC ec particularly esophageal adenocarcinoma eac has been rising dramatically in the usa esophageal squamous cell carcinoma escc is associated with ED and black ethnicity while eac is more common among whites the influence of social ED and ethnicity has not been studied in england west midlands CA intelligence unit data were used to study the incidence of escc and eac and the influence of age sex socioeconomic status townsend quintiles by postcode and ethnicity to individual patients from hospital episode statistics from to a total of ec were identified fiveyear directly age standardized incidence rates per ci for men increased from in to in and for women from to escc incidence did not alter but eac incidence rose rapidly in males to and in females to escc was strongly associated with the most socially deprived quintile eac was not associated with differences in socioeconomic status eac was significantly more common in WM and women when compared with black and asian populations in england the incidence of eac has rapidly risen particularly in men over the last three decades escc was strongly associated with social deprivation eac was more common in WG populations but no association with the SES status was found | MEDAL |
the design of a two year prospective T0 of mothers and their newborn children aiming at determining the incidence of congenital toxoplasmosis in denmark is presented toxoplasma EA in a bloodspot from the P0 routinely collected on filterpaper the guthrie card and EA in a stored SS sample from the mother collected in the first trimester are compared SC identify mothers INF with t gondii during pregnancy and identify newborn children at risk of CT all children at risk will be treated and followed until congenital toxoplasmosis is verified or excluded children with verified congenital toxoplasmosis will be followed clinically and the effectiveness of the treatment will be evaluated the study design allows determination of the maternofetal transmission rate in untreated pregnancies and will furthermore allow a good estimate of the incidence of congenital toxoplasmosis in denmark in addition we hope that the diagnosis and treatment of cases of subclinical toxoplasmosis will prevent chorioretinitis and minor CBF disorders later in life of pregnancies tested during the first month of the study cases of maternal seroconversions have been diagnosed only one child had detectable toxoplasma TPS igm antibodies at birth | MEDAL |
the purpose of this T0 was to estimate the effect of chlorpromazine on free radical concentration in hemndp MC using electron paramagnetic resonance epr spectroscopy it was found that chlorpromazine at concentrations of à and à m contributed to the formation of FRs g values in a dosedependent manner the increase in free radical formation was accompanied by an increase in cytotoxicity as shown by a tetrazolium CA homogeneous broadening of epr lines slow spinlattice relaxation processes and strong dipolar interactions characterized all the tested cellular samples the performed examination of free radical formation in cells exposed to different chlorpromazine concentrations confirmed the usefulness of electron paramagnetic resonance spectroscopy to determine the effect of a drug on free radical production in a cellular MM system in vitro | MEDAL |
the reported biological halflife of the antiarrhythmic drug PA pa is between and h to reassess this the disposition kinetics of the drug and its pharmacologically active metabolite nacetyl PA napa were determined using a newly developed high performance liquid chromatography hplc method the assay involved SE from plasma of pa and napa with methylene chloride followed by a brief washing of the extract with water separation and evaporation of the organic layer reconstitution in the mobile phase watermethanolacetic acidtriethylamine and i.t. into the hplc system at an ultraviolet CS detection wavelength of nm the samples were chromatographed on a reversephase column the minimum quantifiable concentration was microgramml for both the drug and its metabolite with this sensitivity it was possible to measure pa and napa plasma concentrations in samples taken as late as h T3 single mg oral doses of pa hcl to healthy volunteers when only the data points up to h were included in the calculation the drug t was h mean sd and in PA with those reported previously however the t became significantly longer h when the and h data points were also included the t of napa was h and close to the reported values | MEDAL |
a substratesupported rapid evaporation crystallization ssrec method was used to develop a highly nonlinear optical material nndimethylaminonmethylstilbazolium tosylate dast which satisfies the rayleigh scattering requirement for the fabrication of highly transparent composites dast NCs have a second harmonic generation active crystal structure and a high signaltonoise ratio sal nerve input maintaining an upright body position requires not only NM activity but also cardiovascular regulation we postulated that plantar mechanical stimulation might modulate autonomic nervous system activity and thereby impact blood pressure adaptation during standing | MEDAL |
the dynamic metabolism as far as bigger amount of water versus solid tissues in child demand much better skilfulness in calculating liquid input and excretion in intensive care it is very important fact in prevention of life threatening conditions in children and adults especially in conditions with multiorganic disorders because their treatment is reciprocally opposite considering experiences of icu uz gentbelgium we made in paediatric intensive care unit at paediatric clinic in tuzla unique method of monitoring liquid balance in children within specific conditions in which standard method of monitoring just the amount of planed liquid body weight and diuresis is not reliable enough with this new monitoring we prescribe dynamics of parenteral input of crystalloid and colloids as well as peroral income much more precise and much more confidence in positive result and prevention of harder disorders as well in this paper we present our own method of observation as well as results in period july till september we did the intensive volume control in or of total number of patients treated in picu in this period we followed the liquid balance days or hours primary nephrologic diseases were indication for balance in or children while in other or children were other diseases like CF diabetic ketoacidosis chronic SVR diseases the toxic shock syndrome the conditions of intracranial hypertension we notice the signs of threatening renal failure in or of children and signs of manifesting renal failure in or our experiences tell that the model we made is applicable in working conditions of icu evidence of organism responds the planned input is much better and safer than in standard way of observation the possibility of in time intervention against threatening renal failure as well as other organic disorders is much greater we recommended this MM for all life threatening conditions in paediatric intensive care units | MEDAL |
a review of patients with largebowel cancer at a major new zealand hospital showed an excess of rightsided Tc PT in females compared with males these observations stimulated analysis of the national OCR registration data for largebowel cancer for the whole of new zealand in the years to patients rates for each site were calculated and agestandardized using world population figures the findings confirmed that females have a higher incidence of rightsided Tc cancer and males a higher incidence of rectal cancer and showed a significant trend in this direction from the ascending colon toward the rectum the observed differences between males and females suggest differences in the etiology of the RT and should be taken into account in assessing modalities and outcomes of treatment | MEDAL |
earlier data suggest that a polymorphism at the serotonin ht transporter gene httlpr may affect depression particularly in the face of stress due to interactions between ht vulnerability and stress SE however this interaction between ht transporterlinked transcriptional promoter region httlpr ht vulnerability and the affective effects of stress SE has not yet been investigated as participants with shortallele httlpr genotypes may exhibit enhanced ht vulnerability this T0 examines the effects of tryptophan challenge on AS reactivity and performance in healthy participants with ss vs ll genotypes sixteen healthy subjects with homozygotic short alleles ssslg lglg and subjects with homozygotic long alleles lllala of the httlpr were tested in a doubleblind placebocontrolled design under acute stress SE following tryptophan challenge or PL although there were no httlprrelated differences in AS responses significant beneficial effects of tryptophan challenge on mood and AS performance were exclusively found in participants with ss genotypes these findings suggest greater brain ht vulnerability to tryptophan manipulations in participants with ss as compared with ll httlpr genotypes this apparent genetic ht vulnerability may become a meaningful risk factor for depression when BB ht falls below PET need in the face of real severe stressful life events | MEDAL |
this report is concerned with designs for field intervention studies utilized in operations research beginning with an overview of the objectives of operations research the authors go on to review the difficulties associated with using true experimental designs proposed guidelines for designing quasiexperimental studies are then discussed the guidelines focus on using multiple sources of data multiple measurements over time and multiple replications of the T0 intervention finally an example is presented of one ongoing operations research study in sri lanka | MEDAL |
this T0 examined questions concerning differences in the acyl composition of membrane phospholipids that have been linked to the faster rates of metabolic processes in endotherms versus ectotherms in liver kidney heart and brain of the ectothermic reptile trachydosaurus rugosus and the endothermic mammal rattus norvegicus previous findings of fewer unsaturates but a greater unsaturation index ui in CM of the mammal versus those of the reptile were confirmed moreover the T0 showed that the distribution of phospholipid headgroup classes was similar in the same tissues of the reptile and mammal and that the differences in acyl composition were present in all phospholipid classes analysed suggesting a role for the physical over the chemical properties of CM in determining the faster rates of metabolic processes in endotherms the most common phosphatidylcholine pc molecules present in all tissues except brain of the reptile were and whereas AA acid containing pcs were the common molecules in the mammal the most abundant PEs pe used in the tissue of the reptile were and compared to and in the mammal ui differences were primarily due to AA acid found in both pc and pes whereas docosahexaenoic acid was a lesser contributor mainly within pes and essentially absent in the kidney the phospholipid composition of brain was more similar in the reptile and mammal compared to those of other tissues | MEDAL |
caactivated cl current in freshly isolated smooth muscle cells from rat portal vein was studied using the wholecell patchclamp technique simultaneously the freecytosolic ca concentration cai was estimated using emission from the dye indo pretreatment of the cells with amytal and carbonylcyanidemchlorophenylhydrazone which reduced the intracellular adenosine triphosphate concentration was used to weaken the cellular ca homeostatic system cai of treated cells slowly increased during perfusion with an external cacontaining solution this rise in cai gradually G1 a cadependent cl current which allowed the study of the relationship between activation of this current and cai levels the threshold cai for activation of cl channels was around nm and full activation occurred at nm the cai dependence of the cl channels was not changed during application of noradrenaline and did not depend on the membrane potential the gating of cadependent cl channels of rat portal vein myocytes seems to be mainly controlled by intracellular ca | MEDAL |
how is the macaque monkey extrastriate cortex organized is vision divisible into separate tasks such as object recognition and spatial processing each emphasized in a different anatomical stream if so how many streams exist what are the hierarchical relationships among Az the present study approached the organization of the extrastriate cortex in a novel manner a principled relationship exists between cortical function and cortical topography similar functions tend to be located near each other within the constraints of mapping a highly dimensional space of functions onto the 2DE space of the cortex we used this principle to reexamine the PET organization of the extrastriate SC given current knowledge about its topographic organization the goal of the study was to obtain a model of the functional relationships among the visual Az including the number of PET streams into which they are grouped the pattern of informational overlap among the streams and the hierarchical relationships among areas to test each PET description we mapped it to a model SC according to the principle of optimal continuity and assessed whether it accurately reconstructed a version of the extrastriate topography of the models tested the one that best reconstructed the topography included four functional streams rather than two six levels of hierarchy per stream and a specific pattern of informational overlap among streams and Az a TPS mixture of functions was predicted for each visual area this description matched findings in the physiological literature and provided predictions of PET relationships that have yet to be tested physiologically | MEDAL |
platelets are suggested to exacerbate ischemiainduced myocardial injury which has led to the study of various antiplatelet therapies including thromboxane synthetase inhibitors txsi two such agents benzylimidazole and oky reduce IS commensurate with a diminution in serum thromboxane b formation in anesthetized dogs subjected to minutes of CAO followed by hours of reperfusion in contrast PLT depletion with TPS AS does not reduce IS but prevents the cardioprotection afforded by the txsi plateletderived prostaglandin endoperoxides pgg and pgh which cannot be converted to thromboxane a in the inhibited platelet can be transformed to pge and pgd in plasma and to pgi by the blood vessel wall these prostaglandins are considered cardioprotective consequently a low dose of aspirin mgkg given hours before CO was used to selectively block the platelet cyclooxygenase enzyme aspirin by itself does not reduce IS but it suppresses the myocardial salvage induced by oky thus txsi reduce infarct size by plateletdependent aspirinsensitive mechanism that depends on the redirection of plateletderived pgg and pgh to protective metabolites rather than inhibition of thromboxane a per se moreover myocardial salvage induced by the txsi is accompanied by a reduction in neutrophil accumulation in the myocardium as indicated by the C2 of the neutrophilspecific myeloperoxidase enzyme PLT depletion or i.c.v. with aspirin prevents the txsiinduced suppression of PMNL accumulation consequently it is proposed that the prostaglandinmediated protective effects of txsi can be resolved at least in part in terms of a braking action on PMNL activation to prevent leukocytedependent tissue injury | MEDAL |
species of alexandrium produce potent neurotoxins termed paralytic shellfish toxins and are expanding their ranges worldwide concurrent with increases in sea surface temperature the metabolism of molluscs is temperature dependent and increases in ocean temperature may influence both the abundance and distribution of alexandrium and the dynamics of toxin uptake and depuration in shellfish here we conducted a largescale T0 of the effect of temperature on the uptake and depuration of PSTs in three commercial oysters saccostrea glomerata and diploid and triploid crassostrea gigas n per speciesploidy level oysters were acclimated to two constant temperatures reflecting current and predicted climate scenarios and °c and fed a diet including the paralytic shellfish toxinproducing species alexandrium minutum while the oysters fed on a minutum in similar quantities concentrations of the toxin analogue gtx were significantly lower in WA s glomerata and diploid c gigas after days following exposure to a minutum toxicity of triploid c gigas was not affected btures in medialrow anchors | MEDAL |
Subsets and Splits
No community queries yet
The top public SQL queries from the community will appear here once available.