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{
"caption": "Monaural Sound Localization in PET Experiments Performed in the Three Groups of Subjects(A) CBF increases. Activations of the right striate and extrastriate cortices are observed in EBSP but not in the two other groups for the contrast of MSL minus its control task. Upper image series, sagittal slices; lower image series, coronal slices. X and Y coordinates refer to standardized stereotaxic space.(B) Behavioral data. Behavioral results in MSL task (with SE bars). The dashed lines represent the ideal performance, whereas the solid lines indicate the best linear fit to the observed localization performance. Negative angles on the abscissa correspond to the obstructed ear, while positive angles correspond to the unobstructed ear. Note the better performance of the EBSP group compared to the EBNP and SIG.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_10-PMC544927-5-pbiop0030027pg001.jpg"
} | 000200 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
|
{
"caption": "(A-E): Morphology of the secondary invagination sites. Confocal micrograph (A, inverted) of a flat preparation of an embryo stained with phalloidin-rhodamine and light micrographs (B-D) and electron micrograph (E) of transverse sections through prosomal hemi-neuromeres. The midline is to the right. (A) Final pattern of the primary invagination sites in the opisthosomal segments 1 and 2. The invagination sites are arranged in 7 rows. The black dots correspond to the constricted cell processes of the individual precursor groups that are attached to the apical surface (arrow). (B) Morphology of the secondary invagination sites. At 250 hours the secondary invaginating cell groups (asterisks) are still attached to the apical surface. The individual groups are isolated by brighter sheath cells (arrowhead). The primary precursor groups have dissociated (arrow) and form basal cell layers. The longitudinal connective (lc) is already visible at the basal side. (C) The secondary invagination sites (asterisks) loose contact to the apical surface, when the epidermis (arrow) overgrows the ventral neuromeres. (D) After invagination the secondary neural precursors (asterisks) remain attached to each other forming epithelial vesicles. The cell processes run parallel to each other and extend to a lumen (arrow). (E) The cell processes (o) of the invaginating cells of a group are opposed to each other and the lumen between the cell processes is filled with microvilli (arrow). Cell junctions connect the individual processes (arrowheads). lc, longitudinal connective; o2 to o3, opisthosomal hemi-segments 2 to 3.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_10-PMC544935-0-1742-9994-1-3-1.jpg"
} | 000201 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
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{
"caption": "(A-C): Mitotic pattern in the ventral neuromeres after formation of the secondary invagination sites. Flat preparations of embryos stained with phalloidin-rhodamine (red) and anti-Phospho-Histon 3 (green). (A) Only scattered mitotic cells (arrowhead) are present in the ventral neuromeres after invagination of the secondary neural precursors (arrow). The pattern of cell divisions in the cephalic lobe and the prosomal segments at 310 hours of development is representative for the late embryonic stages. (B) Optical section through apical cell layers of the fourth prosomal hemi-neuromere. Only a few mitotic cells (arrowhead) are associated with epithelial vesicles. (C) A similar pattern is visible in basal cell layers of the same neuromere. The arrowhead points to a dividing cell, the arrow points to a dissociating epithelial vesicle. ch, cheliceral neuromere; cl, cephalic lobe; l1 to l2, prosomal hemi-neuromeres corresponding to walking legs 1 to 2; ped, pedipalpal hemineuromere.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_10-PMC544935-1-1742-9994-1-3-6.jpg"
} | 000202 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
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{
"caption": "(A-j): Invagination of secondary neural precursors and formation of epithelial vesicles. (A-F) Confocal micrographs of flat preparations of embryos stained with phalloidin-rhodamine. (B-G) Flat preparations of the fourth prosomal hemi-segments. (A) At 220 hours about 25 secondary invagination sites form (arrow). There is no clear dividing line between the formation of secondary invagination sites (arrow) and invagination of primary neural precursors. Some primary invagination sites are still visible (arrowhead) The bars indicate the segment borders. (B) Apical optical section of the pattern of secondary invagination sites (arrow) at 240 hours of development. (C) Epidermal cells overgrow the ventral neuromeres between 250 and 300 hours (arrowheads) The arrow points to a secondary invagination group. (D) After invagination the individual cells of a groups remain attached to each other forming epithelial vesicles (arrow). (E) At 300 hours the anterior-posterior extension of the individual hemi-segments has been reduced leading to a rearrangement in the positions of the invaginated cell groups (arrow). (F) After 320 hours 8 of the 25 invaginated cell groups are no longer visible indicating that the cells have detached from each other. The arrow points to an invaginated cell group. (G) 10 cell groups are still visible at hatching (arrow). (H) Overview of the arrangement of epithelial vesicles (arrow) of the four prosomal hemi-segments corresponding to the four walking legs. The anterior-posterior reduction in size is clearly visible (compare to A). The bars indicate the segment borders. (I) Flat preparation of the prosoma at hatching. Epithelial vesicles are still visible (arrow). The bars indicate the segment borders, the arrowhead points to the midline. (J) Flat preparation of the brain at 350 hours. The arrow points to epithelial vesicles. ch, chelicera; l1 to l4, prosomal neuromeres corresponding to walking leg 1 to 4; leg 1, walking leg 1.p, pedipalp; ped, pedipalpal neuromere.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_10-PMC544935-4-1742-9994-1-3-4.jpg"
} | 000203 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
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{
"caption": "(A-C): Secondary invagination sites are surrounded by sheath cells. Electron micrographs of transverse sections through prosomal hemi-neuromeres. (A,B) Invagination sites (arrows) are surrounded by sheath cells (asterisks) that appear translucent in the electron microscope. The sheath cells extend processes (arrowhead) that enwrap the individual invagination sites. (C) Sheath cells that are located in the apical cell layer form bizarre shapes that extend into the cell free space at the ventral side of the embryo (arrowhead). The sheath cells are labeled with asterisks, the arrow points to an invagination site.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_10-PMC544935-6-1742-9994-1-3-2.jpg"
} | 000204 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
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{
"caption": "Expression of Gm-dpp in G. marginata embryos. (A) stage 2. The arrow points to expression in the dorsal portion of the neuroectoderm. (B) stage 3. The arrows point to the dorsal and ventral (middle) portion of the neuroectoderm, respectively. (C) stage 3. Aspect of the head. The arrow points to expression in the brain. (D) stage 4. Arrow: expression in the optic lobe. Asterisk: expression in the antennal neuromere. Arrowheads: expression in the heart. (E) stage 5. Arrow: expression in the optic lobe. Arrowheads: expression in the heart. (F) stage 6.1. The arrowheads denote expression in the dorsal portion of the germ band that is probably correlated with heart formation. A-E are in ventral aspect. F is in lateral aspect. Abbreviations: md, mandible; mx, maxilla; an, antenna; t1, t2, t3, first three trunk legs.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_10-PMC544938-2-1742-9994-1-6-2.jpg"
} | 000205 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
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{
"caption": "The relation between the expression of Gm-wg and Gm-Dll. Preparations of maxillae (A, C) and mandibles (B, D) simultaneously labeled with a mixture of probes against Gm-wg and Gm-Dll. In the maxilla the patterns complement each other to stain the entire ventral edge of the appendage, whereas in the mandible no significant difference is observed compared to Gm-wg expression detected alone. Compare to Fig. 4. Stages are as indicated in the top left corner of the panels. Abbreviations see Fig. 3.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_10-PMC544938-3-1742-9994-1-6-5.jpg"
} | 000206 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
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{
"caption": "High titer antinuclear antibodies (ANA) of the homogeneous pattern by indirect immunofluoerescence on immobilized HEp-2 cells in a female patient with autoimmune hepatitis type 1 (AIH-1). Homogeneous ANA are frequently found in AIH-1 (original magnification 40×).",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_10-PMC544946-1-1740-2557-1-2-1.jpg"
} | 000207 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
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{
"caption": "Antimitochondrial antibodies react to the proximal and distal tubules of the rat kidney (original magnification 40 ×). In these cases there is also reactivity to the parietal cells of the rat stomach.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_10-PMC544946-3-1740-2557-1-2-7.jpg"
} | 000208 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
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{
"caption": "Smooth muscle autoantibodies by indirect immunofluoerescence on rat stomach (serum from a female patient with autoimmune hepatitis type 1). The immunofluorescence involves smooth muscle fibers within muscularis mucosa (original magnification 40×).",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_10-PMC544946-4-1740-2557-1-2-4.jpg"
} | 000209 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
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{
"caption": "Smooth muscle antibodies by indirect immunofluoerescence on rat kidney (from a female patient with autoimmune hepatitis type 1). The immunofluorescence involves smooth muscle fibers within blood vessels (original magnification 40×).",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_10-PMC544946-5-1740-2557-1-2-3.jpg"
} | 000210 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
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{
"caption": "Typical staining of antinuclear antibodies in the serum of a patient with autoimmune hepatitis type 1 visualized by indirect immunofluoerescence on cryostat sections of rat liver (original magnification 40×).",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_10-PMC544946-6-1740-2557-1-2-2.jpg"
} | 000211 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
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{
"caption": "Perinuclear staining of anti-neutrophil cytoplasmic autoantibodies (p-ANCA) by indirect immunofluoerescence on ethanol fixed human granulocytes (serum from an ANA negative patient with autoimmune hepatitis type 1). Original magnification 40×).",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_10-PMC544946-7-1740-2557-1-2-5.jpg"
} | 000212 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
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{
"caption": "Staining of the liver of diabetic transgenic PEPCK-Ins mice with haematoxylin and eosin. (Black Bar = 20μm).",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_10-PMC544947-2-1740-2557-1-3-5.jpg"
} | 000213 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
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{
"caption": "A-F. In-situ hybridisation. Pancreatic islets from a diabetic transgenic mouse illustrating insulitis, (A) antisense and (B) sense. Transgenic liver from a diabetic transgenic mouse (C) antisense and (D) sense. Wild type liver from a diabetic NOD mouse (E) antisense and (F) sense.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_10-PMC544947-4-1740-2557-1-3-3.jpg"
} | 000214 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
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{
"caption": "The results of chest HRCT examination in a SARS patient in the convalescent phase, showing marked reversal of pulmonary fibrosis.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_10-PMC545044-0-1465-9921-6-5-1.jpg"
} | 000215 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
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{
"caption": "Low power photomicrograph of the phyllodes tumor: Histologically, the neoplasm was biphasic, with proliferating stroma, compressed glands, and a well developed leaf-like architecture (H&E, original magnification 40×).",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_10-PMC545046-0-1477-7819-2-46-1.jpg"
} | 000216 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
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{
"caption": "Photomicrograph of the DCIS patterns: The DCIS showed cribriform and micropapillary architecture, with necrosis (H&E, original magnification 100×).",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_10-PMC545046-2-1477-7819-2-46-4.jpg"
} | 000217 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
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{
"caption": "High power photomicrograph of the phyllodes tumor: The stroma lacked cellular atypia and mitoses, consistent with a benign phyllodes tumor (H&E, original magnification 400×).",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_10-PMC545046-3-1477-7819-2-46-2.jpg"
} | 000218 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
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{
"caption": "Immunohistochemical staining of VEGF-C in biopsied specimens (upper panel) and hematoxylin-eosin (H&E) staining, immunohistochemical staining of cytokeratin and VEGF-C in metastastic lymph node (middle and lower panel). a, VEGF-C positive type in biopsied specimens. b, VEGF-C negative type in biopsied specimens. c, f, hematoxylin-eosin (H&E) staining in metastastic lymph node. d, g, immunohistochemical staining of cytokeratin in metastastic lymph node. e, VEGF-C positive type in metastastic lymph node. h, VEGF-C negative type in metastastic lymph node.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_10-PMC545047-0-1477-7819-3-2-1.jpg"
} | 000219 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
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{
"caption": "Immuno-histochemistry for I-FABP in colons from AOM-treated rats. Panels A and B are sections from CAS and WPH-fed animals, respectively. Arrows point to the strong areas of staining for I-FABP in the inter-cryptal surface epithelium (overall intensity of staining is greater for CAS than for WPH).",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_10-PMC545049-2-1476-4598-4-1-5.jpg"
} | 000220 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
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{
"caption": "CT scan image of the lung of case 3 patient. A: Before vaccination (May 15, 2003). B: After the third vaccination (June 18, 2003). Rapid growth of the metastatic tumors and pleural effusion were seen.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_10-PMC545052-0-1479-5876-3-1-1.jpg"
} | 000221 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
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{
"caption": "CT scan image of the lung of case 5 patient. A: Before vaccination (July 8, 2003). B: After the first vaccination (July 22, 2003). C: After the third vaccination (August 19, 2003). D: After the sixth vaccination (September 16, 2003). The metastatic tumors appeared to be dormant after the first vaccination.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_10-PMC545052-1-1479-5876-3-1-2.jpg"
} | 000222 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
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{
"caption": "The histological examination of the removed specimen (right hemicolectomy specimen) revealed actinomyces colonies in the upper part of the cecum and the ascending colon wall. The large spherical clusters, densely packed and branching, and PAS positive \"sulfur granules\" are specific of actinomycosis.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_10-PMC545056-0-1471-230X-5-1-2.jpg"
} | 000223 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
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{
"caption": "In abdominal CT scan a large inflammatory tumor like mass is identified. The characteristics of the mass do not indicate colon actinomycosis. The characters of the lesion and the coexisting edema and the severe and diffuse inflammation of the mesentery in the lower right quadrant probably suggest perforated ascending colon tumor.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_10-PMC545056-1-1471-230X-5-1-1.jpg"
} | 000224 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
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{
"caption": "Postsynaptic localization of GluRIIA, GluRIIB, and DLG requires contact by the presynaptic neuron, but localization of DLG does not depend on the presence of glutamate receptors A: Confocal projections of late stage 17 embryonic NMJs visualized using antibodies to the neuronal membrane marker anti-HRP (green) and anti-GluRIIA subunit antibodies (magenta). This image shows NMJs on interior-most ventral longitudinal muscles in two neighbouring hemisegments in a prospero null mutant. The muscles in the upper hemisegment are normally innervated; the muscles in the lower hemisegment are not innervated. Major anatomical landmarks are labelled: In the upper hemisegment, intersegmental nerve branch b (ISNb) enters from the right (medial) and branches to form NMJs on muscles 7 & 6, 13, and 12. ISNb is absent in the uninnervated hemisegment. Note the lack of GluRIIA clusters in this hemisegment. B: Uninnervated muscles 6 & 7 in a prospero mutant embryo, stained using antibodies against GluRIIB, DLG, and the neuronal membrane marker HRP. Note the lack of GluRIIB clusters, and dispersal of DLG throughout the muscle membrane. C: Innervated muscles 6 & 7 in a GluR-less Df(2L)SP22 mutant embryo, stained using antibodies against the synaptic vesicle protein cysteine string protein (CSP, green)) and DLG (magenta). Note that DLG clusters properly at the synapse. Scale bars (A, B, C) = 15 μm.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_10-PMC545058-1-1741-7007-3-1-5.jpg"
} | 000225 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
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{
"caption": "DLG, GluRIIA, and GluRIIB are localized postsynaptically at the Drosophila NMJ A: Confocal projection of two boutons in a Drosophila third instar neuromuscular junction, visualized using the neuronal membrane marker anti-HRP (red) and anti-DLG antibodies (green). Scale bar = 2 μm. B: Isosurface projection generated from the confocal stack shown projected in A. At this stage of development, larval boutons are partially embedded in postsynaptic muscle membrane. DLG immunoreactivity surrounds the boutons, consistent with postsynaptic localization. C, D: Confocal projections of larval NMJs visualized using antibodies that recognize DLG (green) and the glutamate receptor subunits GluRIIA or GluRIIB (magenta, in panels C and D, respectively). Note the incomplete overlap of DLG and glutamate receptors; glutamate receptor immunoreactivity falls within the area stained by DLG, but not all DLG immunoreactivity overlaps with glutamate receptor immunoreactivity. Scale bar = 10 μm.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_10-PMC545058-4-1741-7007-3-1-1.jpg"
} | 000226 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
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{
"caption": "Comparison of the wild type and transgenic Ndn expression profiles in E10.5 and E12.5 mouse embryos. In situ hybridization was performed with an Ndn riboprobe on sagittal sections of wild type (A, B, C, H), Tg92mat-Ndn+/-pat (D, E, F, I) and Ndn+/-pat (G) embryos. The absence of Ndn signal in the E10.5 Ndn+/-pat embryo (G) demonstrates the specificity of the Ndn riboprobe. Similar expression profiles are shown in the infundibulum recess (ir) of E10.5 embryos (A, D), in the preplate of the telencephalic epithelium (pp) and in the dorsal root ganglia (drg) of E12.5 embryos. Note that no hybridization signal is detected in the transgenic Tg92mat-Ndn+/-pat E12.5 muscle (ms) (I) whereas endogenous Ndn is expressed in this tissue (H). rp, rathke pouch; tv, telencephalic vesicle.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_10-PMC545062-1-1471-2156-6-1-5.jpg"
} | 000227 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
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{
"caption": "Confocal images of the distribution pattern of beta1 integrin receptor molecules on HT-29, DanG, and Caki I tumor cells. Integrin subtypes which were predominantly expressed on the respective tumor cell lines are shown. All: magnification × 100.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_10-PMC545066-2-1471-2407-5-4-4.jpg"
} | 000228 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
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{
"caption": "Double staining of a sentinel node for CD4 (brown staining) and CD28 (red staining).1a). Primary follicle showing a high expression of CD28.1b). Primary follicle showing a low expression of CD28. 1c). Germinal center showing a moderate expression of CD28. 1d). Germinal center showing a low expression of CD28. 1e). Paracortical area showing a moderate expression of CD28. 1f). Paracortical area showing a low expression of CD28. 1g). Double staining of a sentinel node for CD3 (brown staining) and the zeta chain (red staining). Paracortical area showing a low expression of the zeta chain. 1h). Double staining of a sentinel node for CD8 (brown staining) and CD28 (red staining). Corresponding paracortical area showing a high expression of CD28.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_10-PMC545070-0-1479-5876-2-45-1.jpg"
} | 000229 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
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{
"caption": "Xmab21l2 rescues dorsal structures in BMP4 injected embryos. (A) As previously described, injection of 0.6 ng of BMP4 mRNA gave rise to ventralized embryos. The most severely ventralized phenotype of BMP4 injection, called bauchstück, was scored with a dorso-anterior index (DAI) of 0 (top), whereas normally dorsalized embryos were assigned a DAI of 5 (bottom). (B) Embryos were injected with 0.6 ng BMP4 (n = 111), with 0.6 ng BMP4 plus 0.8 ng Mab21l2 (n = 83), or with 0.6 ng BMP4 plus 1.6 ng Mab21l2 (n = 101). In each group, we scored the percentage of complete ventralization (DAI = 0, grey bars) and normal dorsal axis formation (DAI = 5, black bars) in embryos that completed development. Intermediate classes are omitted in the plot. Coinjection of embryos with Mab21l2 significantly increased the percentage of correctly dosalized embryos (see text for details). Statistical analysis was conducted using the Chi square algorithm (1 df). *: p = 0.0005; **: p = 0.0087.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_10-PMC545073-3-1471-2121-5-48-3.jpg"
} | 000230 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
|
{
"caption": "Myocardial wall malformations in Ptdsr-/- embryos (a, b) Sagital sections of wild-type (a) and homozygous mutant (b) embryos at 16.5 dpc revealed a thinning of the myocardial wall (compact zone) and an increased myocardial trabeculation (b) in the mutant heart. Scale bar = 100 μm.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_10-PMC545075-0-1471-213X-4-16-9.jpg"
} | 000231 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
|
{
"caption": "Cardiac malformations in Ptdsr-/- embryos: analysis using histology Embryos analyzed by MRI (Figure 3) were sectioned transversely and stained with hematoxylin and eosin. (a–c) Serial caudal to cranial sections of the wild-type embryo showing normal cardiac and vascular anatomy. The left and right ventricles (lv, rv) are separated by the interventricular septum (ivs). The ascending aorta (a-ao) arises from the left ventricle, continues on as the aortic arch (ao-a), which joins the descending aorta (d-ao). The pulmonary artery (pa) arises from the right ventricle via the pulmonary valve (pv) and continues as the arterial duct (ad), which joins the descending aorta. The left and right atria (la, ra), trachea (tr), right main bronchus (rmb) and esophagus (es) are indicated. (d) Section through embryo 33 indicating the ventricular septal defect (VSD). (e, f) Sections through embryo 55 showing that both aorta and pulmonary artery arise from the right ventricle (double outlet right ventricle), and that the arterial duct of the pulmonary artery is narrow in comparison to the aorta – indicating pulmonary artery hypoplasia. The aortic valve (aov) is indicated. (g–i) Serial caudal to cranial sections through embryo 35 showing a VSD, aorta arising from the right ventricle (double outlet right ventricle), and a severely narrowed arterial duct. Scale bars = 500 μm; axes: r – right; l – left; d – dorsal; v – ventral. Individual embryos are indicated by number.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_10-PMC545075-1-1471-213X-4-16-7.jpg"
} | 000232 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
|
{
"caption": "High-throughput high-resolution magnetic resonance microscopy. (a) Stack of 32 embryos embedded in a NMR tube. (b) Section through the long axis of the NMR tube showing embryos in eight layers. (c) Sagittal section through layer 8 showing the four embryos in this layer. (d–f) Transverse, sagittal, and coronal sections through individual embryos in layers 5, 1 and 4 respectively. The voxel size is 25.4 × 25.4 × 24.4 μm. Structures indicated are the spinal cord (sc), the right and left lungs, atria and ventricles (rl, ll, ra, la, rv, lv), primary atrial and interventricular septa (pas, ivs), mitral valve (mv), midbrain roof (mbr), midbrain (mb), mesencephalic vesicle (mes), thalamus (tha), hypothalamus (hy), pons (po), cerebellum (c), medulla oblongata (mo), pituitary (pit), tongue (t), thymus (th), left superior vena cava and main bronchus (lsvc, lmb), aorta (ao), liver (li), stomach (s), left adrenal and kidney (lad, lk), pancreas (pa), intestines (i), umbilical hernia (uh), aqueduct of Sylvius (aq), fourth ventricle (fv), inner ear (ie), larynx (lar), right ventricular outflow tract (rvot), spleen (sp), and testes (te). Scale bars = 500 μm; axes: d – dorsal; v – ventral; r – right; l – left; a – anterior, p – posterior.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_10-PMC545075-2-1471-213X-4-16-1.jpg"
} | 000233 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
|
{
"caption": "Cardiac malformations and thymus hypoplasia in Ptdsr-/- embryos. (a–c) Transverse and oblique (through the plane of the ascending aorta) sections, and 3D reconstruction (left-ventral oblique view) of a heart of a wild-type embryo at 15.5 dpc. The left and right ventricles (lv, rv) are separated by the interventricular septum (ivs). The left and right atria (la, ra), and the trachea (tr) are also indicated. The ascending aorta (a-ao) arises from the left ventricular outflow tract (lvot), via the aortic valve (ao-v), and continues on as the aortic arch (ao-a), which joins the descending aorta (d-ao). The pulmonary artery (pa) arises from the right ventricular outflow tract (rvot), and continues as the arterial duct (ad), which joins the descending aorta. (d–f) Corresponding images of a Ptdsr-/- embryo, showing a smaller heart with a ventricular septal defect (VSD). The aorta arises from the right ventricle. The pulmonary artery is small and its connection to the descending aorta (arterial duct) could not be identified. (g–i) Corresponding images of another Ptdsr-/- embryo, showing a ventricular septal defect (VSD). The aorta overrides the VSD resulting in a double-outlet right ventricle. (j, k) Coronal sections of Ptdsr+/+ and Ptdsr-/- embryos, showing the two lobes of the thymus (th). The arterial duct of the pulmonary artery in the Ptdsr-/- embryo is narrowed. (l) Correlation between embryo weight and volume. Scattergram of embryo weight versus embryo volume measured from multi-embryo MRI datasets for 16 embryos using Amira. The co-efficient of regression (r) is indicated. (m, n,) Absolute embryo and thymus volumes (μl) were measured from the MRI datasets from 5 wild-type (wt), 3 heterozygote (h), and 8 Ptdsr-/- (m) embryos at 15.5 dpc. There was no significant difference in the wild-type and heterozygote data, which were therefore pooled together (wt/h). (o) Relative thymus volumes (% of embryo volume) were calculated as Ptdsr-/- embryos were slightly smaller than littermate wild-type embryos. The data are represented as mean ± S.D. The probability of a type I error (P) is indicated. Scale bars = 317 μm; axes: r – right; l – left; d – dorsal; v – ventral; a – anterior, p – posterior. Individual embryos are indicated by number.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_10-PMC545075-3-1471-213X-4-16-6.jpg"
} | 000234 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
|
{
"caption": "Analysis of Ptdsr expression in the embryonic heart (a, b) Staining of heterozygous Ptdsr-βgeo-embryos [21] using X-Gal at 10.5 dpc (a) and 11.5 dpc (b). (a) At 10.5 dpc Ptdsr expression can be seen throughout the heart. (b) Transverse sections of X-Gal stained embryos at 11.5 dpc showed an increased expression of Ptdsr in the myocardial wall and a beginning decrease of the expression in the trabeculation. Scale bar = 100 μm in (b).",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_10-PMC545075-4-1471-213X-4-16-8.jpg"
} | 000235 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
|
{
"caption": "Identification of cardiac malformations in Ptdsr-/- embryos using multi-embryo MRI (a–e) Transverse thoracic sections showing the heart of heterozygous or wild-type control embryos from each litter. The left and right ventricles (lv, rv) are separated by the interventricular septum (ivs). The left and right atria (la, ra) are also indicated, separated by the primary atrial septum (pas). (f–i) Corresponding sections through littermate Ptdsr-/- embryos, showing ventricular septal defects (VSD). Scale bar = 635 μm; axes: d – dorsal; v – ventral; r – right; l – left; a – anterior, p – posterior. Individual embryos are indicated by number.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_10-PMC545075-5-1471-213X-4-16-5.jpg"
} | 000236 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
|
{
"caption": "Identification of septal, outflow tract, and aortic arch malformations using multi-embryo MRI (a – e') Images of transverse sections from 5 Cited2-/- embryos obtained using the multi-embryo technique (a–e) compared with images from the same embryos obtained subsequently using the single embryo technique (a'–e'). (a, a') Section showing left and right atria and ventricles (la, ram, live, rave). The atria are separated by the primary atria septum (pas), which is deficient at its ventral margin creating an osmium premium type of atria septal defect (ASD-P). (b, b') Section showing a ventricular septal defect (VSD) in the interventricular septum (ivs). (c, c') Section showing double outlet right ventricle, wherein the ascending aorta (a-ao) and the pulmonary artery (pa) both arise from the right ventricle (rv). The aortic valve (ao-v) is indicated. (d, d') Section showing a right-sided aortic arch (ao-a) passing to the right of the trachea (tr) and the esophagus (es). (e, e') Section showing bilateral aortic arches (ao-a) forming a vascular ring around the trachea (tr) and the esophagus (es). Also indicated are the thymus (th) and the right superior vena cava (r-svc). (f – j) Serial transverse sections through a wild-type heart obtained using single embryo MRI, demonstrating corresponding normal structures, including the systemic venous sinus (svs), left superior vena cava (l-svc), pulmonary vein (pvn), descending aorta (d-ao), mitral and tricuspid valves (mv, tv), the secondary atrial septum (sas), left and right ventricular outflow tracts (lvot, rvot), pulmonary valve (pv), and arterial duct (ad) of the pulmonary artery. Scale bars = 635 μm for multi-embryo, and 317 μm for single embryo images; axes: d – dorsal; v – ventral; r – right; l – left.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_10-PMC545075-6-1471-213X-4-16-2.jpg"
} | 000237 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
|
{
"caption": "Identification of adrenal agenesis using multi-embryo MRI Images of coronal sections from 2 embryos obtained using the multi-embryo technique (a, b) compared with images from the same embryos obtained subsequently using the single embryo technique (a', b'). (a, a') Normal right adrenal gland (rad) anterior to the right kidney (rk) in a wild-type embryo. The right lung (rl) is indicated. (b, b') Agenesis of right adrenal gland in a Cited2-/- embryo. Scale bars = 635 μm for multi-embryo, and 317 μm for single embryo images; axes: d – dorsal; v – ventral; a – anterior, p – posterior.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_10-PMC545075-7-1471-213X-4-16-3.jpg"
} | 000238 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
|
{
"caption": "Edema in Ptdsr-/- mice (a) The Ptdsr-/- mutant (15.5 dpc) is growth retarded and the severe edema along the back of the embryo is visible. (b, c) Sagital sections of embryos at 16.5 dpc. The mutant embryo (c) exhibits massive subcutaneous edema compared to a wild-type (b) littermate. Scale bar = 100 μm in (b) and (c).",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_10-PMC545075-8-1471-213X-4-16-4.jpg"
} | 000239 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
|
{
"caption": "Section of the corneal button from a case of bacterial keratitis showing epithelial ulceration. Note the dense the inflammatory infiltrates in the anterior two-thirds of corneal stroma and the quiet deep corneal stroma. (Hematoxylin and eosin, ×125)",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_10-PMC545077-0-1471-2415-4-16-1.jpg"
} | 000240 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
|
{
"caption": "A low power picture of corneal button with Acanthamoeba keratitis showing inflammation in the superficial layers with few keratocytes in the deeper stroma. (Hematoxylin and Eosin, ×100) [Left]. TUNEL stained sections of the same case show dense diffuse brown staining in the inflammatory region in the anterior stroma while the deeper stroma shows brown positivity in the few remaining keratocytic nuclei. (TUNEL ×100) [Right]",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_10-PMC545077-1-1471-2415-4-16-3.jpg"
} | 000241 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
|
{
"caption": "The corneal stroma in a case of fungal keratitis shows diffuse TUNEL positivity in the zone of inflammation (*) as well as discrete positive staining in the peripheral keratocytes away from zone of inflammation (arrow head) (TUNEL, ×250) [Left]. The higher magnification of the same shows TUNEL positive nuclei of the keratocyte (Arrow) in a clean background, free of inflammatory cells. (TUNEL staining, ×500) (TUNEL ×400) [Right]",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_10-PMC545077-2-1471-2415-4-16-4.jpg"
} | 000242 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
|
{
"caption": "Section from a case of viral keratitis showing TUNEL staining in the epithelial cells. The underlying stroma shows inflammatory cells and irregular keratocytic nuclei. (TUNEL, ×500)",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_10-PMC545077-3-1471-2415-4-16-5.jpg"
} | 000243 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
|
{
"caption": "The section from a case of Acanthamoeba keratitis showing cysts of Acanthamoeba in the deep stroma. Note the complete absence of inflammatory cells in this area as well as the loss of keratocytic nuclei. (Hematoxylin and eosin, ×500)",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_10-PMC545077-4-1471-2415-4-16-2.jpg"
} | 000244 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
|
{
"caption": "Ray patterns of C. briggsae and C. elegans. Ventral views of a) C. elegans and b) C. briggsae male tails. Anterior to the left. Left side up. Bilateral pairs of rays are numbered from anterior to posterior. a) C. elegans pattern in which ray 3 is separate from all other rays. This pattern is referred as a 2(1)3+3 pattern. b) C. briggsae pattern in which ray 3 is clustered with rays 4 – 6. This pattern is referred to as a 2/4+3 pattern. In this pattern ray 3 may be either free (right side) or fused with ray 4 (left side). The 2(1)3+3 pattern is ancestral to the Elegans-group, a monophyletic clade that includes C. elegans and C. briggsae [34].",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_10-PMC545079-0-1471-2148-5-3-1.jpg"
} | 000245 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
|
{
"caption": "T2-Weighted Magnetic Resonance Imaging Showing Blood in the Arterial Wall and Narrowing of the Lumen of the Left Internal Carotid ArteryThis is also known as the “crescent sign,” a hallmark of internal carotid artery dissection.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_10-PMC545208-0-pmedp0020019pg003.jpg"
} | 000246 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
|
{
"caption": "Assessing lung tumors for gene mutations could help guide therapy",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_10-PMC545210-0-pmedp0020021pg001.jpg"
} | 000247 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
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{
"caption": "Replication timing of human artificial chromosomes in line 17-B12. BrdU detection (red) in cells that have been blocked with colcemid in mitosis following BrdU pulses during S phase (see Materials and methods). Artificial chromosome (small arrows; enlarged artificial chromosomes are shown in inserts) and chromosome 17 (large arrow) locations in each spread were confirmed by FISH analyses using a D17Z1 probe (data not shown). (a-d) Images from different periods in S phase. (a) Early in S phase, at 0-2 h, the two artificial chromosomes present in this spread are not replicating. Some incorporation of BrdU on chromosome 17 is detectable. (b) In the middle of S phase, at 2-4 h, two of four artificial chromosomes are replicating. (c) Later, at 4-6 h, all three artificial chromosomes are being coordinately replicated. Some BrdU incorporation within chromosome 17 arms is detectable. (d) Late in S phase, at 6-8 h, artificial chromosomes are not replicating. The centromere region on chromosome 17 is replicating (large arrow). Because of the A-rich sequence composition of satellite III on Yq, BrdU is preferentially incorporated into one strand, producing an asymmetrical staining pattern on Yq (arrowheads) [84].",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_10-PMC545780-0-gb-2004-5-11-r89-4.jpg"
} | 000248 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
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{
"caption": "Transcriptionally competent chromatin is present on artificial chromosomes. Dimethylation of lysine 4 on histone H3 (H3DimK4) was visualized using an antibody against H3DimK4 (red). This euchromatin mark was detected on all artificial chromosomes (arrowheads) generated from either D17Z1 in lines (a) 17-D34, (b) 17-E29, (c) 17-B12 and (d) 17-C20, or DXZ1 in lines (e) X-4 or (f) X-5. Host centromere regions were generally depleted for H3DimK4 as indicated by arrows pointing to centromere regions of chromosome 17 (a-d) and the X chromosome (e, f).",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_10-PMC545780-1-gb-2004-5-11-r89-3.jpg"
} | 000249 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
|
{
"caption": "Detection of HP1α on D17Z1-based artificial chromosomes. (a-d) Cell lines stably expressing a Myc-tagged form of HP1α. HP1α was detected using an anti-Myc antibody (red). The artificial chromosomes (about 1-3 Mb; indicated by small arrows) in lines (a) 17-D34-1.A2 and (b) 17-E29-1.C23 exhibit faint HP1α staining at a level similar to the general arm staining. Larger artificial chromosomes (3-10 Mb; small arrow) in lines (c) 17-C20-1.B22 and (d) 17-B12-1.B10 stain strongly for HP1α. Inserts in (a-c) show either DAPI (blue)-stained artificial chromosomes or HP1α (red). Host 17 centromere regions are indicated by the large arrows in (a-c). In (d), simultaneous staining for CENP-A (green) shows that CENP-A is restricted to a portion of the artificial chromosome (arrows) whereas the HP1α signal coats the entire artificial chromosome. In contrast to CENP-A, which is present at comparable levels on all artificial chromosomes tested [12,13,58] and host kinetochores [61], HP1α staining levels are more variable at host centromere regions (d).",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_10-PMC545780-2-gb-2004-5-11-r89-2.jpg"
} | 000250 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
|
{
"caption": "Replication timing in different human artificial chromosomes. (a-c) Detection of BrdU (red) on artificial chromosomes (small arrows; larger version in inserts). (a) In mid S phase, at 2-4 h, two artificial chromosomes in line 17-D34 are BrdU positive. (b) The artificial chromosome in line 17-E29 is replicating early in S phase, in the 0-2 h period. (c) In mid S phase (2-4 h), three artificial chromosomes are being coordinately replicated in this spread from line 17-C20. Images shown are from the first half of S phase, and, as expected, Yq (arrowhead) is not replicating at this time.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_10-PMC545780-3-gb-2004-5-11-r89-5.jpg"
} | 000251 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
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{
"caption": "Heterochromatin forms on artificial chromosomes in the 3-20 Mb size range but is depleted on smaller artificial chromosomes that are approximately 1-3 Mb. Indirect immunofluorescence using an antibody that recognizes modification of histone H3 by trimethylation at lysine 9/lysine 27 (H3TrimK9/K27) (red signal) demonstrated that these heterochromatin markers are not detectable on the smaller D17Z1-based artificial chromosomes (arrowheads) in lines (a) 17-D34 and (b) 17-E29, but are readily detectable on the larger D17Z1- and DXZ1-based artificial chromosomes (arrowheads) as shown in lines (c) 17-B12, (d) 17-C20, (e) X-4 and (f) X-5. Arrows indicate chromosome 17 centromere regions (a-d) or host X centromere regions (e, f). Host D17Z1 sequences typically stained positive for H3TrimK9/K27 in most spreads (arrows in a-d). It was difficult to detect the X centromere signal (for example, arrow in (e)) but in about 30% of spreads there was a clearly positive signal as indicated by the arrow in (f). (g) Variation in H3TrimK9/K27 levels at host centromere regions is shown in a larger area of the spread shown in (c): artificial chromosomes are indicated by arrowheads; arrows point to the consistently strongly positive signals on the long arm of the Y chromosome (Yq). Artificial chromosome size estimates are listed in Table 2. Confirmation of artificial chromosomes and relevant host centromere regions were determined by FISH analyses with appropriate alpha-satellite probes (data not shown).",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_10-PMC545780-4-gb-2004-5-11-r89-1.jpg"
} | 000252 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
|
{
"caption": "Targeting of GFP-MRP7 and GFP-GSA to peroxisomes in yeast cells. Fluorescence of CB80 yeast cells expressing (a) GFP and DsRed-SKL; (b) GFP-SKL and DsRed-SKL; (c) GFP-MRP7 and DsRed-SKL; or (d) GFP-GSA and DsRed-SKL. Transformed cells were cultured on oleate and observed live for fluorescence. Control experiments (a) show that GFP co-localizes with Ds-Red-SKL only when the sequence -SKL is appended at its extreme carboxyl terminus (b). The figures reveal a punctuate fluorescence pattern for GFP fused to the yeast mitochondrial ribosomal protein L2 encoded by MRP7 (c) or to the bacterial enzyme glutamate-1-semialdehyde 2,1-aminomutase (GSA) (d). Both fusion proteins co-localize with DsRed-SKL in yeast peroxisomes. GFP fused to GSA without its carboxy-terminal -AKL gave rise to a diffuse (cytosolic) fluorescence pattern (data not shown).",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_10-PMC545800-0-gb-2004-5-12-r97-2.jpg"
} | 000253 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
|
{
"caption": "Targeting of GFP-tyrosinase and GFP-lysozyme to peroxisomes in human cells. Fluorescence of human COS7 cells expressing (a) GFP-lysozyme or DsRed2-SKL; (b) GFP-tyrosinase and DsRed2-SKL; or (c) GFP-lysozyme and DsRed2-SKL. Cells were observed 36 h after transfection (magnification 60 ×). Separate small images of the GFP fluorescence (green) and DsRed2 fluorescence (red) are shown to the left of each main picture, in which the two fluorescent images are overlaid. Areas in which red and green fluorescence coincide show as yellow. (a) Control experiments reveal that expression of GFP-lysozyme is an adjunct to the cellular punctuate fluorescence pattern independently of the presence of DsRed2-SKL. The figures show a punctate fluorescence pattern for GFP fusions with (b) human tyrosinase and (c) chicken lysozyme. Both proteins co-localize with DsRed2-SKL in human peroxisomes as demonstrated by the fluorescence overlay. Owing to the evolutionary conservation of PEX5 within the metazoans [7,13,33], a chicken protein (lysozyme) can be assayed in a human cell line and the species barrier is not an issue in this study.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_10-PMC545800-2-gb-2004-5-12-r97-1.jpg"
} | 000254 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
|
{
"caption": "Reduction of cell surface heparan sulfate glycosaminoglycans significantly reduces intracellular lipid accumulation in adipocytes. 3 mM 4-MUmb or pNP-Xyl was added to 3T3-L1 cells concurrently with differentiation reagents as in Fig. 5. Cells were then fixed with 10% formaldehyde, stained with 0.1% Oil Red O and photographed with phase contrast optics (A; magnification, 400×). (B), after image capture, cell-associated Oil Red O was extracted with 0.1 N HCl and dye was quantitated by spectrophotometry. Asterisk indicates a statistically significant difference compared to untreated adipocytes (p < 0.01).",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_10-PMC545935-5-1476-511X-4-2-6.jpg"
} | 000255 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
|
{
"caption": "Bronchoscopic secretion quantification card.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_10-PMC545936-0-1465-9921-6-3-1.jpg"
} | 000256 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
|
{
"caption": "APV inhibited Aβ uptake, neuronal C1q production, and microglial activation. Slices were treated with no peptide (a, b, c), 30 μM Aβ 42 (d, e, f), or 30 μM Aβ 42 + 50 μM APV (g, h, i) for 3 days with fresh reagents added daily. Immunohistochemistry for Aβ (4G8, a, d, g), C1q (anti-rat C1q, b, e, h), and microglia (CD45, c, f, i) was performed on fixed and sectioned slices. Scale bar = 50 μm. Results are representative of three separately performed experiments. j. Immunoreactivity of Aβ (open bar), C1q (black bar), or CD45 (striped bar) was quantified as described in Materials and Methods. Values are the mean ± SD (error bars) from images taken from 8 slices (2 sections per slice) in 3 independent experiments (* p < 0.0001 compared to Aβ, Anova single factor test).",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_10-PMC545941-0-1742-2094-2-1-1.jpg"
} | 000257 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
|
{
"caption": "Aβ10–20 blocked Aβ42 uptake, microglial activation, but not neuronal C1q induction. Slices were treated with no peptide (a, b, c), 10 μM Aβ 42 (d, e, f), 10 μM Aβ 42 + 30 μM Aβ 10–20 (g, h, i) or 30 μM Aβ 10–20 (j, k, l) for 3 days with fresh peptides added daily. Immunohistochemistry for Aβ (4G8, a, d, g, j), C1q (anti-rat C1q, b, e, h, k), and microglia (CD45, c, f, i, l) was performed on fixed and sectioned slices. Results are representative of three independent experiments. Scale bar = 50 μm. m. Immunoreactivities of Aβ (open bar), C1q (black bar), or CD45 (striped bar) were quantified as described in Materials and Methods. Values are the mean ± SD (error bars) from images taken from 8 slices (2 sections per slice) in 3 independent experiments. Microglial activation by Aβ42 was significantly inhibited by Aβ10–20 (* p < 0.0001, compared to either Aβ42 + Aβ10–20 or Aβ10–20, Anova single factor test).",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_10-PMC545941-5-1742-2094-2-1-5.jpg"
} | 000258 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
|
{
"caption": "Detection of MT expression by immunohistochemistry. Intense membranous, cytoplasmic and nuclear staining in a case of clear cell type RCC (x330).",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_10-PMC545945-0-1477-7819-3-5-1.jpg"
} | 000259 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
|
{
"caption": "Phase-contrast photomicrographs of fragile X progenitor cells The figure shows clusters/spheres during the initial stages (2–3 days after plating) of adherence to a fibronectin substrate. (A) 4×; (B) 10×; (C) 20×. Confluent serum- and growth factor-expanded cultures were serum deprived for one week in the presence of growth factors, then lifted with enzyme-free buffers and transferred to new plates with no fibronectin substrate. After growing the resulting clusters/spheres for two weeks, the clusters/spheres were transferred to new fibronectin-coated plates. Clusters/spheres (black arrows) are abundant and are seen adhering to the substrate. Cells (black arrowheads) can be seen streaming from the spheres and spreading out on the substrate.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_10-PMC545950-0-1471-2350-6-2-1.jpg"
} | 000260 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
|
{
"caption": "Wild-type Drosophila nerve cord (left) showing expression of the Nplp1 neuropeptide in a small subset of neurons. Combinatorial misexpression (right) of regulatory genes triggers ectopic Nplp1 expression in neurons throughout the nervous system.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_100-PMC1790956-0-pbiop0050051pg001.jpg"
} | 000261 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
|
{
"caption": "Tricuspid valve vegetation measuring 1.14 cm × 1.12 cm in this\nview.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_100-PMC1791015-0-IDOG2006-43253p001.jpg"
} | 000262 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
|
{
"caption": "Vulvar abscess (The lesion is 5 × 4 cm in size,\nred, and swollen).",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_100-PMC1791056-0-IDOG2007-63565p001.jpg"
} | 000263 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
|
{
"caption": "Transmission electron micrographs reveal the various stages of apparent endocytosis of the NBISA01 virus in Atlantic salmon erythrocytes. (A) a virus particle within an invagination of the plasma membrane (bar = 139 nm); (B) partial closure of the pit containing a virus particle (bar = 139 nm); (C) and (D) virus particle with in a vesicle in the erythrocyte cytoplasm (bar = 139 nm).",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_100-PMC1793955-1-1743-422X-4-13-1.jpg"
} | 000264 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
|
{
"caption": "siRNA-mediated EHD protein knock-down effects on transferrin loading in HeLa cells. (A) HeLa cells were seeded on autoclaved glass coverslips in 6-well plates for 24 h followed by transfection of 200 pmol of double-stranded RNA oligonucleotides with irrelevant or EHD siRNA for 48 h prior to transferrin loading. Cells were starved for 30 min in starvation media followed by Transferrin-coupled Alexa Fluor 594 in internalization buffer at 37°C for 15 min, washed with ice-cold PBS, fixed and scanned using a confocal microscope equipped with a 40× objective lens. The arrows in the EHD1 siRNA depict an ERC transferrin loading phenotype while the arrows in the EHD4 siRNA depict an EE phenotype. (B) Cells were transfected with siRNA for EHD proteins for 24 h and further transfected with Rab5-GFP or Rab11-GFP for an additional 24 h. Cells were then loaded with labeled transferrin for 15 min as described in Methods. Arrowheads point to colocalized structures. Bar, 10 μm. Data are representative of 3 individual experiments.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_100-PMC1793994-0-1471-2121-8-3-10.jpg"
} | 000265 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
|
{
"caption": "Myc-EHD1 ΔEH and EHD3 ΔEH cause perinuclear clustering of Rab11-GFP. HeLa cells were co-transfected with Myc-EHD ΔEH proteins (red) and Rab11-GFP (green) for 24 h, fixed, stained with antibodies for Myc (9E10), mounted and scanned by a confocal microscope equipped with a 100× objective lens.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_100-PMC1793994-1-1471-2121-8-3-8.jpg"
} | 000266 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
|
{
"caption": "Differential subcellular localization patterns of human EHD proteins. (A) HeLa cells were transfected with C-terminal GFP-tagged EHD proteins for 24 h, fixed, mounted and scanned by a confocal microscope equipped with a 100× objective lens. Human EHD1-, 3- or 4-GFP are localized on tubular and vesicular structures in the perinuclear area, while EHD2-GFP is seen only in vesicular structures. Cells expressing EHD2 show microspikes; t, tubule; v, vesicle; m, microspikes. (B) HeLa cells were transfected with C-terminal GFP-tagged EHD ΔEH mutants for 24 h, fixed, mounted and scanned by a confocal microscope equipped with a 100× objective lens. Bar, 10 μm.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_100-PMC1793994-11-1471-2121-8-3-5.jpg"
} | 000267 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
|
{
"caption": "All human EHD proteins rescue the vacuolated intestinal phenotype in the intestine of C. elegans rme-1 (b1045). (A) Micrograph images of middle intestinal regions of transgenic animals expressing the human EHD proteins. The rme-1(b1045) worms were injected with pVha-6::SL2-GFP (50 ng/μl) or with the same construct containing the human EHD cDNAs along with myo2::GFP (100 ng/μl) as a co-injection marker. Intestinal vacuoles are viewed as spaces devoid of green fluorescence in the rme-1(b1045) mutant (arrows). (B) Intestinal vacuoles were counted in at least 3 independent transgenic lines expressing no vector (rme-1(b1045)), vector alone (Vector), or a vector containing EHD1-4. (C) Basolateral endocytosis assay of the intestinal vacuoles. Adult hermaphrodites were microinjected with 1 mg/mL Texas-Red BSA (TR-BSA) into the pseudocoelom and examined for uptake in intestinal vacuoles. Lack of accumulation of TRed-BSA microinjected into the pseoudocelum in wild-type (WT) worms (N2 Bristol strain) (left). Rapid accumulation of TR-BSA in the enlarged intestinal vacuoles (arrows) in the rme-1(b1045) mutant worms (middle). rme-1(b1045) worms rescued with human EHD4 do not display accumulation of the dye in any intestinal cells (right) similar to WT animals. * – pseudocoelom, ** – gonads. DIC – differential interference contrast microscopy.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_100-PMC1793994-3-1471-2121-8-3-1.jpg"
} | 000268 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
|
{
"caption": "Colocalization of EHD proteins with the endocytic recycling marker Rab11. HeLa cells were co-transfected with Rab11-GFP (green) and EHD-DsRed (red) proteins for 24 h, fixed, mounted and scanned by a confocal microscope equipped with a 100× objective lens. Colocalization is indicated when similar shaped structures appear yellow in the Merge (arrowheads). Bar, 10 μm.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_100-PMC1793994-4-1471-2121-8-3-7.jpg"
} | 000269 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
|
{
"caption": "Light micrographs of transverse section of mouse aorta from wild-type (panel A) and fibulin-4+/− (panel B). Sections were stained for collagen (C), elastin (E) and smooth muscle cell nuclei (SM).",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_100-PMC1794134-1-ponep0000229pg001.jpg"
} | 000270 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
|
{
"caption": "Electron micrographs of transverse ultra-thin sections of aortas from fibulin-4+/− mice housed in a standard cage (panel A) and an enriched cage (panel B).",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_100-PMC1794134-2-ponep0000229pg002.jpg"
} | 000271 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
|
{
"caption": "Electron micrographs of transverse ultra-thin sections of aortas from wild-type mice housed in a standard cage (panel A) and an enriched cage (panel B).",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_100-PMC1794134-6-ponep0000229pg003.jpg"
} | 000272 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
|
{
"caption": "Expression of markers for subcellular structures in retinal ganglion cells.(A) Single confocal image plane of a large ON ganglion cell transfected with expression plasmids for EGFP-F (labeling the cell membrane) and DsRed2-ER (labeling the endoplasmic reticulum.) Scale bar, 50 µm. (B) Volume reconstruction of a confocal image stack of a large ganglion cell transfected with the cell-filling marker HcRed and a marker for postsynaptic densities of inhibitory synapses, gephyrin-GFP (green). Scale bar, 50 µm. (C) A single dendrite of an OFF alpha ganglion cell transfected with gephyrin-GFP. Note the high density of puncta particularly on the proximal dendrites. Scale bar, 20 µm.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_100-PMC1794165-0-ponep0000221pg005.jpg"
} | 000273 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
|
{
"caption": "Patch-clamp recordings and light responses from ganglion cells in the incubated retina.(A) HcRed-transfected ON-type retinal ganglion cells after 3 day incubation. The patch-clamp pipette is seen (its light responses shown in C). Black dots are unincorporated gold particles. Scale bar, 50 µm. (B) An example of voltage clamp and current clamp recordings after 6 day incubation. All of recorded ganglion cells (n = 30) generated action potentials in response to current injection under current clamp configuration. In the voltage clamp, the cells were voltage-clamped at −77 mV and then command pulses were applied from −117 mV to 13 mV, in 10 mV steps, for 200 msec. In the current clamp, command pulses were applied from −40 pA to +180 pA, in 20 pA steps, for 200 msec. Scale bars are 50 msec. (C–E) light responses of ganglion cells from 3 day (the cell shown in A), 4 day, and 6 day-incubated retina, respectively, recorded under current clamp.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_100-PMC1794165-2-ponep0000221pg003.jpg"
} | 000274 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
|
{
"caption": "Pre- and postsynaptic markers and ankyrin-G are correctly localized in transfected retinal neurons.(A) High-power view of several dendrites of a retinal ganglion cell transfected with PSD95-GFP (green) and counterstained with an antibody against the synaptic ribbon component Kif3a (red). Most PSD95 puncta are in close proximity to one or more synaptic ribbons. Scale bar, 10 µm. (B) Ankyrin-G-MB-GFP (green) and HcRed (red) were transfected into a retinal ganglion cell. HcRed labels the whole extent of the cell, whereas Ankyrin-G-MB-GFP is found in the initial segment of the axon (arrows). Some Ankyrin-G-MB-GFP labeling is seen in the soma and the primary dendrites, the place of protein synthesis. Scale bar 50 µm. (C) Starburst amacrine cell transfected with an expression plasmid for synaptophsin-GFP. The known synaptic output sites of these cells, varicosities in the outer third of the dendritic tree are labeled with this marker. (D) Starburst amacrine cell transfected with expression plasmids for the cell-filling marker HcRed (red) and a fusion protein from the synaptic vesicle marker VAChT-GFP (green). Scale bar, 50 µm.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_100-PMC1794165-5-ponep0000221pg006.jpg"
} | 000275 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
|
{
"caption": "Immunohistochemistry of lungs on day 7 after infection with HMPV or RSV. (A) Normal lung tissue from an uninfected animal. (B) Pulmonary section from an RSV-infected mouse showing mild bronchopneumonia with scattered macrophages and neutrophils in alveolar spaces. (C) Severe bronchopneumonia in a mouse inoculated with HMPV. Bronchioli and adjacent alveoli are densely infiltrated by macrophages and neutrophils admixed with fibrin. (D) Immunohistochemical staining for HMPV. Groups of intraalveolar macrophages and pneumocytes expressing HMPV antigens. (A to D): hematoxylin-eosin staining, original magnification × 20; D: immunostaining with anti-HMPV serum, (× 63). Representative sections from groups of 4 mice are shown.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_100-PMC1794227-2-1465-9921-8-6-2.jpg"
} | 000276 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
|
{
"caption": "Microscopy image of a multicellular tumor spheroid, exhibiting an extensive branching system that rapidly expands into the surrounding extracellular matrix gel. These branches consist of multiple invasive cells. (Reprinted from Habib et al. [33], with permission from Elsevier).",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_100-PMC1794228-0-1742-4682-4-4-1.jpg"
} | 000277 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
|
{
"caption": "Scanning electron micrographs of biofilm formation. (a) F. solani (238-06) incubated with fresh ACUVUE lens and balanced salt solution for 48 h at 25°C on a rotator (180 rpm). (b) Close-up of the same isolate showing hyphal attachment to the lens surface (c) Disappearance of biofilm when preparation from (a) was treated with MoistureLoc for 4 h.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_100-PMC1794232-2-1471-2415-7-1-4.jpg"
} | 000278 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
|
{
"caption": "Acute LV systolic dysfunction in a 50-year old female patient affected by granulocytic sarcoma during CT by daunorubicine (50 mg/m2), cytarabine (30 mg/m2), etoposide 50 mg/m2. LV end-diastolic diameter (left panel) is 48.9 mm and LV end-systolic diameter (right panel) is 41.7 mm. Thus, endocardial fractional shortening is 14.7 % and EF = 37.9 %. EF = Ejection fraction, LV = Left ventricular.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_100-PMC1794233-1-1476-7120-5-4-2.jpg"
} | 000279 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
|
{
"caption": "Doppler transmitral inflow pattern in a 58-year old female patient with acute myeloid leukemia before treatment (upper panel) and after DNR (= daunorubicine) 50 mg/m2, VP16 (= etoposide) 50 mg/m2, ARA-C (= cytarabine) 30 mg/m2 (lower panel). Transmitral pattern, normal before treatment (transmitral E/A ratio = 1.12, deceleration time = 172 ms), shifts to pattern of LV abnormal relaxation, corresponding to grade I of LV diastolic dysfunction (E/A ratio = 0.70, deceleration time = 230 ms) after CT.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_100-PMC1794233-2-1476-7120-5-4-3.jpg"
} | 000280 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
|
{
"caption": "Detection of posterior pericardial effusion in a 28-year old female patient affected by non Hodgkin's lymphoma and treated by CHOP (Cyclophosphamide 750 mg/m 2 + Adriamycin 50 mg/m 2 + Vincristine 2 mg + Prednisone 100 mg) and previous mediastinal radiation therapy. The usual grading of pericardial effusion takes into account the diastolic separation between epicardium and pericardium: 1. small = < 10 mm corresponding to 300 ml, 2. moderate = 10–20 mm corresponding to 500 ml, 3. severe = > 20 mm corresponding to > 700 ml). In this patient the diastolic separation is < 10 mm (both in long and in short-axis vies), indicating mild pericardial effusion. Upper panel; visualization of diastolic pericardial effusion in parasternal long-axis (left panel) and short-axis view (right panel). Lower panel: visualization of systolic pericardial effusion in parasternal long-axis (left panel) and short-axis view (right panel).",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_100-PMC1794233-3-1476-7120-5-4-4.jpg"
} | 000281 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
|
{
"caption": "Ultrasound evidence of lung water (\"comet tail\") of V inter-costal space in a 58-year old male patient with pulmonary microcitoma after CT including carboplatinum, etoposide and radiation therapy.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_100-PMC1794233-5-1476-7120-5-4-6.jpg"
} | 000282 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
|
{
"caption": "Cre-mediated RB ablation results in abnormal development of small intestine. Haematoxylyn-Eosin (H/E) staining was performed on intestine sections including duodenum (A, E, I), jejunum (B, F, J) and ileum (C, G, K) as well as cross sections (D, H, L) derived from RB+/+ (A, B, C, D), RB+/- (E, F, G, H) and RB-/- (I, J, K, L) newborn mice.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_100-PMC1794236-0-1471-213X-7-6-1.jpg"
} | 000283 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
|
{
"caption": "Loss of pRb stimulates Indian hedgehog expression. (A) RT-PCR from P0 intestines derived from RB+/+, RB+/- and RB-/- newborn pups were performed to detect the expression levels of Shh, Ihh, Ptch1, Ptch2, Gli1, Gli2, Gli3 and actin used as a control. (B) Immunofluoresence analysis of Ihh in intestine sections derived from RB+/+, RB+/- and RB-/- newborn pups was performed by using anti-Ihh antibody. Arrows indicate regions of increased anti-Ihh immunostaining.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_100-PMC1794236-1-1471-213X-7-6-5.jpg"
} | 000284 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
|
{
"caption": "Disruption of pRb induces cell proliferation. The embryonic intestine sections (E17.5) derived from RB+/- (A, B) or RB-/- (C, D) mice were stained by IHC with proliferating cell marker Ki67. The number of proliferative cell in RB null intestine was highly increased. An enlargement of the portion in the left-hand micrograph (A, C) is shown in the right-hand (B, D) micrograph. BrdU positive cells in embryonic intestine sections derived from RB+/+ (E, H), RB+/- (F, I) and RB-/- (G, J) mice were stained with anti-BrdU antibody conjugated with Alexa Fluor 488 (E, F, G) or DAPI (H, I, J).",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_100-PMC1794236-2-1471-213X-7-6-2.jpg"
} | 000285 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
|
{
"caption": "Lack of RB stimulates enterocytes, goblet, enteroendocrine and Paneth cells. Representative sections of duodenum from RB+/+ (A, D, G), RB+/- (B, E, H) and RB-/- (C, F, I) newborn pups were stained with Alkaline phosphatase (A, B, C), Alcian blue (D, E, F) and IHC using anti-serotonin antibody (G, H, I). Nuclear fast red was used as a counterstain for AP and Alcian blue staining. The quantified activity of Alkaline phosphatase was measured by the density of image (J). Alcian blue positive cells (K) or serotonin positive cells (L) were counted in 100 villi derived from RB+/+, RB+/- and RB-/- intestines. Small intestine from newborn pups was subjected to RT-PCR using primers specific to cryptdin-1 and actin as a control (M). The amount of cryptdin-1 was evaluated through the density of signal (N).",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_100-PMC1794236-3-1471-213X-7-6-3.jpg"
} | 000286 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
|
{
"caption": "Ectopic Fgf-signaling represses foxi1 and dlx3b before and during gastrulation. (A, B, F, G) Expression of foxi1 and dlx3b is absent after overexpression of fgf8 by mRNA injections at the 1-cell stage in comparison to wild-type embryos. (C, D, H, I) Misexpression of fgf8 at 30% epiboly or shield stages results in smaller foxi1 and dlx3b expression domains. (E, J) Loss of Fgf-signaling after pharmacological inhibition with SU5402 from late blastula stages until the end of gastrulation has no effect on the ventral expression of foxi1 and dlx3b. Lateral views at the end of gastrulation with dorsal to the right and anterior towards the top. Scale bar: 100 μm.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_100-PMC1794237-1-1471-213X-7-5-2.jpg"
} | 000287 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
|
{
"caption": "Bmp-signaling is required for foxi1 and dlx3b expression during gastrulation. (A-C, F-H) Expression of foxi1 and dlx3b is strongly reduced in bmp7 mutants or embryos injected with Vox and Vent morpholinos (MOs) in comparison to wild-type embryos. (D, E, I, J) Overexpression of bmp2b by mRNA injections at the 1-cell stage leads to an expansion of both foxi1 and dlx3b expression domains at the expense of anterior neural plate. (A-D, F-I) Lateral views at the end of gastrulation with dorsal to the right and anterior upward; (E, J) dorsal views of embryos shown in D and I with anterior towards the top. Scale bar: 100 μm",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_100-PMC1794237-2-1471-213X-7-5-3.jpg"
} | 000288 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
|
{
"caption": "foxi1 and vent but not dlx3b are direct targets of Bmp-signaling during gastrulation. (A, B, E, F, I, J) Expression of foxi1, dlx3b and vent is expanded after Bmp2a protein injection at late blastula stages in comparison to wild-type embryos. (C, D, G, H, K, L) Pharmacological inhibition of protein synthesis with cycloheximide (CHX) at late blastula stages blocks all indications of foxi1, dlx3b and vent expression (C, G, K), whereas foxi1 and vent but not dlx3b expression is restored in CHX-treated embryos after Bmp2a protein injection (D, H, L). Lateral views at the end of gastrulation with dorsal to the right and anterior towards the top (note that CHX treatment blocks epiboly movements). Scale bar: 90 μm.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_100-PMC1794237-3-1471-213X-7-5-4.jpg"
} | 000289 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
|
{
"caption": "Ectopic otic induction after fgf8 expression at late gastrulation stages requires both, Foxi1 and Dlx3b. (A-D) Inactivation of Dlx3b or Foxi1 in wild-type embryos by morpholino injection (MO) leads to a reduction of ear size in comparison to wild-type embryos, and combined loss of Dlx3b and Foxi1 results in loss of all indications of otic specification. (E-H) Ear size reduction by depletion of Dlx3b or Foxi1 but not combined loss of Dlx3b and Foxi1, can be partially rescued in transgenic hsp:fgf8 embryos heat shocked at late gastrulation stages. Lateral views of otic vesicles hybridized with starmaker at 24h with anterior to the left and dorsal towards the top. Scale bar: 40 μm.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_100-PMC1794237-5-1471-213X-7-5-7.jpg"
} | 000290 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
|
{
"caption": "Otic vesicle size is affected by the overexpression of fgf8 depending on the developmental stage. (A, B) Overexpression of fgf8 by mRNA injections at the 1-cell stage completely ablates all indications of otic fate (27/40 embryos, B) in comparison to wild-type vesicles (A). (C-E) Misexpression of fgf8 at 30% epiboly (28/28 embryos, C), shield stage (27/28 embryos, D) and 75% epiboly (29/29 embryos, E) results in smaller otic vesicles. (F-H) Otic vesicles are increased in size if fgf8 is misexpressed at the tailbud (26/29 embryos, F) or the 4-somite stage (24/28 embryos, G) whereas at the 8-somite stage no change in vesicle size is observed (26/26 embryos, H). Lateral views of otic vesicles highlighted with starmaker at 24h with anterior to the left and dorsal towards the top. Scale bar: 30 μm.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_100-PMC1794237-6-1471-213X-7-5-1.jpg"
} | 000291 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
|
{
"caption": "Ectopic otic induction after fgf8 expression at late gastrulation stages leads to formation of larger but correctly patterned placodes and vesicles. (A, E) Otic placodes labeled by cldna expression are increased in size in transgenic hsp:fgf8 embryos in comparison to non-transgenic embryos following a heat shock at late gastrulation stages. (B-D, F-H) The enlarged otic vesicles of transgenic embryos show no apparent patterning defects in comparison to non-transgenic siblings assessed by morphology and marker gene expression, including otx1 and pax2a. (A, E) Dorsal views of 12-somite stage embryos with anterior towards the top; (B-D, F-H) lateral views of otic vesicles at 24h with anterior to the left and dorsal towards the top. o, otolith. Scale bar: 50 μm for A, E; 30 μm for B-D, F-H.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_100-PMC1794237-7-1471-213X-7-5-6.jpg"
} | 000292 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
|
{
"caption": "Ectopic foxi1 expression after treatment with retinoic acid (RA) results in ectopic Fgf-dependent otic induction. (A, B, E, F) In comparison to wild-type control embryos treated with DMSO, embryos treated with 20nM RA show ectopic pax8 and foxi1 expression surrounding the anterior neural plate border without affecting the neural expression of otx2. (C, G) In fgf3, fgf8 double mutants, pax8 is completely abolished in the control embryos, whereas RA-treated double mutant embryos show weak anterior expression of pax8. (D, H) In foxi1 mutants treated with DMSO, pax8 expression can not be detected in the preotic region; but in foxi1 mutants treated with RA, residual anterior pax8 expression is present. Dorsal views of 1–3-somite stage embryos with anterior towards the top. Scale bar: 40 μm.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_100-PMC1794237-8-1471-213X-7-5-8.jpg"
} | 000293 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
|
{
"caption": "Section demonstrating an intravascular proliferation of uniform spindle cells. (H&E ×10).",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_100-PMC1794242-0-1477-7819-5-7-2.jpg"
} | 000294 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
|
{
"caption": "MRI of the right hand showing a pseudosarcomatous mass encircling the ulnar artery.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_100-PMC1794242-1-1477-7819-5-7-1.jpg"
} | 000295 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
|
{
"caption": "Morphology of cultured adult rat RGCs. The cells, cultured for 3 (A & B), 7 (C & D), and 11 (E & F) days, were labeled with anti-Thy-1 antibody (red) and DAPI nuclear stain (blue). (B), (D), are (F) are the corresponding phase-contrast images. Scale bar = 50 μm.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_100-PMC1794249-1-1471-2202-8-11-2.jpg"
} | 000296 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
|
{
"caption": "Effect of glutamate on RGC morphology and survival. Cells were treated with glutamate or vehicle for 3 days. (A and B) Morphology of RGCs with glutamate (100 μM) treatment. (A) The cells were labeled with anti-Thy-1 antibody (red) and DAPI nuclear stain (blue). (B) The corresponding phase-contrast image. (C) Effect of glutamate (100 μM) on RGC survival (n = 76). *** represents P < 0.001 vs. the control group by Student's t-test. (D) Concentration-dependent toxicity of glutamate on cultured RGCs (n = 6). * represents P < 0.05 vs. the control group by One-way ANOVA then Dunnett's test. (E) Effect of MK801 on glutamate-induced RGC toxicity. Cells were treated with the indicated concentrations of MK801 in the presence of 100 μM of glutamate (n = 6). * represents P < 0.05 vs. the glutamate alone group by One-way ANOVA then Dunnett's test. Cell survival in (C), (D), and (E) was assessed by manually counting the Thy-1-positive cells. Data are presented as mean ± SEM.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_100-PMC1794249-2-1471-2202-8-11-3.jpg"
} | 000297 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
|
{
"caption": "Segmentation of wild type and Kv1.1 null hippocampus and ventral cortex. The borders of the hippocampus (red) were drawn in three dimensions. Examples are shown for coronal (A, F), sagital (B, G) and horizontal (C, H) planes. The segmentation resulted in a 3D surface reconstruction of the hippocampus (E, J). The ventral cortex volume was derived from ventral cortex area (blue) measured in four coronal sections evenly distributed from 1.2 to 2.5 mm posterior to Bregma (D, I). Note the difference in size between wild type (top, i.e. A, B, C, D, E) and Kv1.1 null (bottom, i.e. F, G, H, I, J).",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_100-PMC1794250-1-1471-2202-8-10-2.jpg"
} | 000298 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
|
{
"caption": "The S-phase fraction of cells increases with loss of an extra 3p21 copy. Living cells of the MCHs #3/KH3 and Δ3/KH39 were incubated with BrdU in final concentration 30 μg/ml during 1 hour followed by fixation with methanol: acetic acid (3:1). FISH with biotin-labeled 3p21-specific RP6-123i13 probe and centromere-specific probe was performed to analyze the #3/KH3 and the Δ3/KH39 respectively. The probe signal was developed using Cy3-conjugated streptavidin (red), while FITC-conjugated anti BrdU antibodies (Roche Molecular Biochemicals Mannheim, Germany) identified cells in S-phase (green). Percentage of S-phase cells in subpopulations of cells with different 3p21 copy numbers in the #3/KH39 and with different chr3 copy number (counted by number of centromeres) in the Δ3/KH39 was calculated in more than 100 nuclei. The calculation was repeated three times when number of cells allowed. The average values and standard deviations are shown.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_100-PMC1794251-1-1471-2407-7-21-6.jpg"
} | 000299 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
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