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{
"caption": "Loss of Kcnq1 expression in marginal cells. The time course of marginal cell reorganization during development was determined by confocal immunohistochemistry of Kcnq1 (red) and phalloidin-staining of α-actin (green) of stria vascularis in whole-mounts. Top, images from Slc26a4+/- mice of various ages. Bottom, images from Slc26a4-/- mice. Note that marginal cells of Slc26a4+/- mice expressed Kcnq1 homogeneously in their apical membrane and that apical membrane surface areas displayed little variation in size. Marginal cells of Slc26a4-/- mice at P10 and P15 expressed Kcnq1 homogeneously, too, and apical membrane surface areas showed little variation in size. However, surface areas were larger compared to Slc26a4+/- mice. At P37, expression of Kcnq1 in Slc26a4-/- mice was reduced in some marginal cells and maintained in others. Loss of Kcnq1 expression correlated with an enlargement of the apical membrane surface area and maintenance of Kcnq1 expression correlated with a reduction in the apical membrane surface area. This segregation of marginal cells was more drastic at P96. The scale bar shown in the bottom right image represents 10 μm and pertains to all images in this figure.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_101-PMC1796619-7-1741-7015-4-37-2.jpg"
} | 000400 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
|
{
"caption": "phAE87Δ17mt3 is viable but has shorter tails that its parent phage. Electron micrographs of representative particles of phAE87 (A) and phAE87Δ17mt3 (B). The average tail length is 200 ± 10 nm for phAE87 and 180 ± 6 for phAE87Δ17mt3. The tail lengths of 30 individual phage particles for each phage were measured.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_101-PMC1796659-4-mmi0062-1569-f6.jpg"
} | 000401 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
|
{
"caption": "Podocalyxin's C-terminal DTHL motif is required for recruitment of NHERF-1.Projections of apical confocal images of transfected MCF-7 cells showing ectopic Podocalyxin (red), endogenous NHERF-1 (green), and DAPI (blue) labeling. Isotype control sample is Podocalyxin-transfected cells labeled with anti-Podocalyxin (red) and NHERF-1 isotype control (green) to demonstrate lack of non-specific NHERF-1 staining. White numbers represent Pearson's colocalization coefficient. Scale bar: 5 µm.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_101-PMC1796660-0-ponep0000237pg007.jpg"
} | 000402 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
|
{
"caption": "Podocalyxin's extracellular domain and the first four amino acids of its cytoplasmic tail are necessary and sufficient for microvillus formation.(A) TEM images of MCF-7 cells transfected with vector or full-length and mutant Podocalyxin. Vertical slices are shown near the apical cell surface with some of the numerous additional microvilli labeled with red arrows. Scale bar: 1 µm. (B) SEM images at the apical surface of transfected MCF-7 cells with microvilli evident as thin surface projections. Scale bar: 2 µm. (C) Microvilli in six 50 µm2 fields were enumerated and graphed. Averages are shown; error bars represent standard deviation. T-tests were used to show statistically significant differences between vector and Podocalyxin-transfected cells, and between wildtype Podocalyxin and ΔEC-transfected cells with p<0.003 in all cases. Representative of two independent experiments.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_101-PMC1796660-3-ponep0000237pg008.jpg"
} | 000403 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
|
{
"caption": "Podocalyxin-induced microvilli are structurally dependent on intact actin filaments.MDCK (A) and MCF-7 (B) cells transfected with murine Podocalyxin or empty vector were treated with the actin disrupting agent latrunculin A (LatA) or DMSO (negative control) and then fixed. Podocalyxin was labeled with an anti-Podocalyxin antibody (green); f-actin was labeled with phalloidin (red), and nuclei were labeled with DAPI (blue). Scale bar: 5 µm.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_101-PMC1796660-7-ponep0000237pg002.jpg"
} | 000404 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
|
{
"caption": "Apical localization of Podocalyxin and NHERF-1 is not dependent on ezrin.MCF-7 cells stably expressing ectopic murine Podocalyxin were either transfected with VSV-tagged dominant negative (N-terminal) ezrin after formation of monolayers and appropriate localization of Podocalyxin and NHERF-1 (A, B) or transfected with VSV-tagged N'ezrin and then replated before immunostaining (C, D). (A–D) Immunolabeling of VSV-tagged N'ezrin (green), ectopic Podocalyxin (red), and endogenous NHERF-1 (blue). Purple represents apical colocalization of Podocalyxin and NHERF-1 in cells not expressing dominant negative ezrin (internal negative control); white represents apical colocalization of all three molecules in cells expressing dominant negative ezrin. Scale bars: 5 µm. (A, C) Projections of merged confocal stacks taken near the apical cell surface showing individual colors and merged images; (B, D) vertical slices of confocal stacks.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_101-PMC1796660-8-ponep0000237pg005.jpg"
} | 000405 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
|
{
"caption": "Podocalyxin induces apical recruitment of f-actin and ezrin, and all three molecules colocalize in microvilli.Projections of apical confocal images of MCF-7 cells transfected with murine Podocalyxin or empty vector showing ectopic Podocalyxin (blue), endogenous ezrin (green), and f-actin (red) labeling. White represents colocalization of all three molecules. Note the long extended microvilli in the Podocalyxin-transfected sample. Scale bars: 5 µm.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_101-PMC1796660-9-ponep0000237pg004.jpg"
} | 000406 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
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{
"caption": "Tumor cells showing immunoreactivity to (a) MIB-1 and (b) EMA (×400).",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_101-PMC1796851-1-1746-1596-2-3-4.jpg"
} | 000407 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
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{
"caption": "Photomicrograph of the tumor showing sheets of tumor cells arranged in papillary pattern and a papilla with fibrovascular core (inset) (H&E, ×200).",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_101-PMC1796851-2-1746-1596-2-3-3.jpg"
} | 000408 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
|
{
"caption": "MRI scan showing a large bifrontal hyperintense mass with central necrosis.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_101-PMC1796851-3-1746-1596-2-3-1.jpg"
} | 000409 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
|
{
"caption": "Magnetic resonance imaging showed the tumor in the left lateral segment of the liver, and a multiple, small-to-moderate nodules in the enlarged spleen.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_101-PMC1796852-0-1746-1596-2-5-1.jpg"
} | 000410 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
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{
"caption": "Morphology of Splenic marginal zone lymphoma. Characteristic micronodular pattern in the SMZL centred in the white pulp, with variable red pulp infiltration (H&E, original magnification ×100).",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_101-PMC1796852-2-1746-1596-2-5-3.jpg"
} | 000411 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
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{
"caption": "Morphology of Splenic marginal zone lymphoma. Small lymphocytes, marginal zone cells and cells resembling monocytoid cells (H&E, original magnification ×400).",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_101-PMC1796852-3-1746-1596-2-5-4.jpg"
} | 000412 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
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{
"caption": "Morphology of Splenic marginal zone lymphoma. Tumor cells express CD79a (EnVision Plus, original magnification ×200).",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_101-PMC1796852-4-1746-1596-2-5-5.jpg"
} | 000413 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
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{
"caption": "The liver tumor showed a classic hepatocellular carcinoma arranged in trabecular and acinar patterns. H&E, original magnification, ×200.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_101-PMC1796852-5-1746-1596-2-5-2.jpg"
} | 000414 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
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{
"caption": "Endoscopic identification of the left recurrent laryngeal nerve (RLN), upper (UPG) and lower (LPG) parathyroid glands.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_101-PMC1796854-2-1471-2482-7-2-2.jpg"
} | 000415 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
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{
"caption": "Architecture of the motor cortex in SALS and control samples. Nissl staining of representative SALS samples (D2, D4, D5) and controls (H1, H3, H4) shows the intact architecture of the motor cortex in samples chosen for expression analysis. All samples are shown at identical magnification. Cell layers are color-coded as indicated for sample D2. ML – molecular layer; EGL – external granular layer; EPL – external pyramidal layer; IGL – internal granular layer; IPL – internal pyramidal layer; MFL – multiform layer; WM – white matter. The scale bar indicates 500 μm.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_101-PMC1796866-0-1471-2164-8-26-1.jpg"
} | 000416 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
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{
"caption": "Selective expression of proprioceptor markers in the DRG and trigeminal system. The sensory ganglia of wild-type embryos were examined at E13.5 and E16.5 for the expression of transcription factors Brn3a, Etv1, Runx3 and Islet2. (a-d) At E13.5, Etv1 expression is restricted to the DRG, while Runx3 is expressed in both the DRG and TG. (e-h) At E16.5, Etv1 is expressed in both the DRG and the TG, but in the TG Etv1 positive neurons with large nuclei consistent with 1a proprioceptors are rare (large arrow). Instead, the majority of Etv1-positive neurons in the TG have nuclei of intermediate size and co-express Islet2; similar cells are also found in the DRG (small arrows). The 1a proprioceptors of the DRG co-express Brn3a, but at relatively low levels. (i) Etv1 expression in the mesV of an E18.5 embryo expressing a tauLacZ transgene integrated into the Brn3a locus [42], which is thus heterozygous for Brn3a, but phenotypically normal. Numerous neurons that co-express Etv1 and the Brn3a-LacZ marker are noted. The caudal location and large size of these neurons are consistent with proprioceptors innervating the muscles of mastication. Cb, cerebellum; chp, choroid plexus; fr, fasciculus retroflexus; IP, interpeduncular nucleus; mes5, mesencephalic trigeminal. Scale 50 μm (a-h), 400 μm (i), 50 μm (i, inset).",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_101-PMC1796875-0-1749-8104-2-3-2.jpg"
} | 000417 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
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{
"caption": "Target genes with decreased expression in the DRG of Brn3a null mice. (a) In situ hybridization confirms decreased expression of multiple Brn3a downstream targets. (b) Immunofluorescence for the galanin neuropeptide shows marked reduction in the dorsal horn and dorsal root entry zone of Brn3a null DRG. All views show lower cervical (brachial) level cross sections of E13.5 embryos. Scale: 200 μm (a), 100 μm (b).",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_101-PMC1796875-2-1749-8104-2-3-4.jpg"
} | 000418 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
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{
"caption": "Target genes with increased expression in the DRG of Brn3a null mice. (a) In situ hybridization confirms increased expression of Msc (musculin), NeuroD1, and Htr3a (serotonin receptor 3A) transcripts in the DRG of Brn3a wild-type and knockout embryos. Note that the expression of Msc in the surrounding musculature (arrows) is unchanged. (b) Immunofluorescence for the somatostatin-14 peptide shows increased expression concentrated in the dorsal root entry zone (arrows). The distribution of somatostatin-14 also reveals an abnormal accumulation of axons in the superficial dorsal horn in the Brn3a null mutant. All views show lower cervical (brachial) level cross-sections of E13.5 embryos. Scale: 200 μm (a), 50 μm (b).",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_101-PMC1796875-3-1749-8104-2-3-3.jpg"
} | 000419 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
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{
"caption": "Representative cervical sections from non-pregnant (NP) (A-B), term pregnant (TP) (C-D) and postpartum (PP) groups (E-F), showing the the expression and distribution of p38MAPK in different cells by using phospho-antibodies. Stroma and blood vessels (A, C and E). Glandular epithelium and subepithelial mucosa area of stroma (B, D and F).",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_101-PMC1796879-1-1477-7827-5-3-2.jpg"
} | 000420 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
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{
"caption": "Representative sections showing the expression and distributions of c-Fos and c-Jun proteins (AP-1) in different cell types of cervical samples from non-pregnant (NP), term pregnant (TP) and postpartum (PP) groups. Stroma and blood vessels for c-Fos shown in in A, C, E and for c-Jun shown in G, I, K (magnification vein). Glandular epithelium and subepithelial mucosa area of stroma, shown for c-Fos in B, D, F and for c-Jun in H, J, L.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_101-PMC1796879-3-1477-7827-5-3-4.jpg"
} | 000421 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
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{
"caption": "Representative sections showing the expression and distributions of pERK and pJNK in different cell types of the uterine cervix by using phospho-antibodies, as shown in non-pregnant (NP) and postpartum (PP) groups. Cervical stroma, blood vessels shown in A, C, E, G. Glandular epithelium and subepithelial mucosa area of stroma shown in B, D, F and H.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_101-PMC1796879-5-1477-7827-5-3-1.jpg"
} | 000422 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
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{
"caption": "Immunohistochemical and RT-PCR analysis of the fra-1 gene expression in fine needle aspiration biopsy samples (FNAB). The cytological specimens investigated by immunohistochemistry were also analyzed by RT-PCR. The mRNAs were extracted from FNABs of breast tissues and amplified by RT-PCR using fra-1 specific primers as specified under \"Methods\". The cDNAs were co-amplified with gapdh gene as an internal control. A) Immunostaining in a FNAB deriving from a normal breast tissue (100 ×), and from a carcinoma samples (100 ×) (B). C) A carcinoma FNAB was incubated without the primary antibody (100 ×) as control of the specificity of the antibodies. D) Fra-1 expression was analyzed by RT-PCR. The sources of RNAs were: lane 1: normal breast; lane 2, breast dysplasia; lanes 3, 4 and 5: breast carcinomas; lane -, water control. Molecular markers are indicated.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_101-PMC1796888-0-1471-2407-7-17-6.jpg"
} | 000423 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
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{
"caption": "Immunohistochemical analysis of FRA-1 protein in breast tissues. Immunostaining of a normal breast tissue (100 ×) (A) and of a ductal carcinoma (200 ×) (B). The same ductal carcinoma was immunostained in the absence of the primary antibody (200 ×) (C), or with the FRA-1 antibodies pre-incubated with the peptide against which the antibodies were raised (200 ×) (D).",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_101-PMC1796888-1-1471-2407-7-17-1.jpg"
} | 000424 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
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{
"caption": "Immunohistochemical analysis of FRA-1 in benign and malignant breast tissues. Paraffin sections from hyperlastic and neoplasic breast tissues were analyzed by immunohistochemistry using antibodies raised against a specific FRA-1 peptide.A) Nuclear and cytoplasmic staining in a typical breast hyperplasia (200 ×). B) Immunostaining of a breast fibroadenoma (200 ×) showing nuclear and cytoplasmic staining. C) and D) Immunostaining of an atypical hyperplasia at two different magnification (100 ×) and (200 ×) respectively: the staining is present in the majority of the nuclei and is weakly present in the cytoplasm. E) Immunostaining of a ductal in situ breast carcinoma (400 ×): the immunostaining is restricted to the nucleus and weakly present in the cytoplasm, but it is not present in all the cells. F) Immunostaining of a lobular carcinoma (400 ×): the immunostaining is restricted to the nucleus, and is present in all malignant cells.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_101-PMC1796888-2-1471-2407-7-17-2.jpg"
} | 000425 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
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{
"caption": "Effects of liarozole fumarate (R85246) in combination with tamoxifen on N-methyl-N-nitrosourea (MNU)-induced mammary carcinoma and uterus in the rat model. Immunohistochemical staining for PCNA in the uterus of rats obtained from OVX controls (A), 17β-estradiol 10 μg/kg (B), liarozole 80 mg/kg (C), tamoxifen 1 mg/kg (D) and liarozole 80 mg/kg + tamoxifen 1 mg/kg (E). Note the inhibitory effect of liarozole significantly negated the tamoxifen stimulatory effect on the PCNA staining (magnification, ×400).",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_101-PMC1796889-3-1471-2407-7-26-4.jpg"
} | 000426 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
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{
"caption": "Examples of amounts of fat in the lumbar multifidus muscles as seen on axial T1- weighted magnetic resonance imaging scans. These were rated as grade 0 if normal condition; grade 1 for slight fat infiltration (10–50%), and grade 2 for severe fat infiltration (>50%).",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_101-PMC1796893-1-1741-7015-5-2-2.jpg"
} | 000427 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
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{
"caption": "The positioning of the axial images. Only the three lower levels were included in the analyses.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_101-PMC1796893-2-1741-7015-5-2-1.jpg"
} | 000428 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
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{
"caption": "Immunohistochemical staining for IL-4, IL-5, IL-13, and IFN-γ of control mice (Group 1), latex challenged mice (Group 2), and latex challenged treated with curcumin (Group 3). A. IL-4; B. IL-5; C. IL-13; and D. IFN-γ.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_101-PMC1796894-1-1476-7961-5-1-5.jpg"
} | 000429 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
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{
"caption": "Histology of the lungs studied from control and experimental mice. A. Lungs of the control mice stained by Hematoxylin and eosin (H&E) ×40. B. Lung tissues stained with PAS ×40. C. Latex challenged mice, H&E at ×40. D. Latex challenged mice at ×400. E. Latex challenged mice stained with PAS at ×400. F. Latex challenged mice stained with PAS at ×400. G. Lung section from curcumin treated mice (Group 3), H&E at ×40. H. Lung sections from curcumin treated mice but magnification H&E at ×400. I. Lung section from curcumin treated mice stained with PAS at ×40.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_101-PMC1796894-4-1476-7961-5-1-4.jpg"
} | 000430 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
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{
"caption": "Maturation of Human NSC-Derived Neurons Based on the Elaboration of Axons, Synapses, and Innervation by Host Neurons(A) This photograph was taken through the ventral horn of a HNu/70 kDa neurofilament protein stained section 3 mo postgrafting and shows bundles of human 70 kDa neurofilament protein (+) axons (indicated with white arrows) originating in HNu (+) grafts (one indicated with an asterisk on top right) and coursing together (red arrows on bottom left) toward the ventral white matter.(B) This photograph shows an NSC graft in the ventral horn of a human Syn-stained section three months postgrafting. The sharp colocalization of Syn (+) puncta with the graft region (boundaries demarcated with arrows) is due to the selectivity of the antibody for human, but not rat, Syn protein.(C and D) These images (C, epifluorescence; D, confocal) were taken from triple-stained sections with HNu (red), TUJ1 (blue), and the presynaptic marker Bsn (green). The Bsn antibody used here recognizes rat and mouse, but not human, protein. (C) depicts a dense field of rat Bsn (+) terminals in proximity to HNu and TUJ1 (+) profiles. Examples of contacts between rat terminals and NSC-derived neurons are shown with arrowheads in the inset, which is a magnification of the profile at the center of the main image. The very large number of such terminals on NSC-derived cell bodies is best illustrated with confocal microscopy (D).(E and F) These photographs (E, epifluorescence; F, confocal) were taken from sections stained with HNu (red), TUJ1 (blue), and mixed VGLUT1/VGLUT2 antibodies (green) and show the innervation of HNu and TUJ1 (+) cells by glutamatergic terminals putatively originating in the host.Scale bars: (A) 80 μm; (B) 20 μm; (C–F) 10 μm.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_101-PMC1796906-0-pmedp0040039pg006.jpg"
} | 000431 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
|
{
"caption": "In Vitro Differentiation of Human NSCs Used for Transplantation(A) The vast majority of cells express the NSC-specific marker nestin (red) immediately before grafting. The DNA dye DAPI (blue) was used to reveal all cells in culture.(B and C) At 14 days within the differentiation phase (i.e., after bFGF removal), ~ 50% of cells acquire MAP2 immunoreactivity and neuronal cytology, with characteristic processes (red, B). A smaller number of cells differentiate into GFAP (+) astrocytes (green, C).(D) Real-time RT-PCR data showing increased neurotrophic factor and NRG expression in the course of NSC differentiation in vitro. The number of days on top of the columns is the days NSCs have been in a phase of differentiation (after withdrawal of fibroblast growth factor). Results are expressed as fold increases compared to levels expressed at the proliferation phase (day 0), the latter values designated as 1. Data represent average ± standard deviation of triplicate measurements of a representative cell culture sample at a given time point. The experiment was repeated twice with different sets of cell samples and yielded very similar results.Scale bars: 50 μm.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_101-PMC1796906-1-pmedp0040039pg001.jpg"
} | 000432 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
|
{
"caption": "Innervation of Host Motor Neurons by Graft-Derived Nerve Cells as Shown on Sections Stained with Human Syn (Red) and TUJ1 (Green) and Studied with Epifluorescence or Confocal MicroscopyHost motor neurons are depicted as large TUJ1 (+) cell bodies, and NSC-derived terminals are labeled with human Syn antibodies.(A) This epifluorescence image shows the site of the original graft (arrow in lower left) and two synaptic fields with host motor neuron pools marked as (1) and (2), with respectively higher and lower density of synaptic appositions. The low-density field (2) is further enlarged in the inset.(B) This confocal image shows, in great detail, a large number of somatic and dendritic terminals from graft-derived nerve cells on a host motor neuron.Scale bars: (A) 200 μm; (B) 20 μm.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_101-PMC1796906-2-pmedp0040039pg007.jpg"
} | 000433 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
|
{
"caption": "Differentiation of Human NSCs into Neurons after Transplantation into the Lumbar Spinal Cord of Normal Adult Sprague-Dawley RatsOutlined areas in (A) and (C) are enlarged in (B) and (D). All images illustrate the neuronal differentiation of NSCs two months postgrafting based on dual-label immunofluorescence for HNu (red) and a neuronal marker (green, representing TUJ1 and NeuN in [A and B], and [C and D], respectively). The predominance of double-labeled profiles in both (B) and (D) (indicated with asterisks) matches the avid neuronal differentiation of human NSCs in nude rats as illustrated in Figure 3. Single HNu-labeled profiles (D) are shown with arrowheadsScale bars: (A and B) 20 μm; (C and D) 10 μm.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_101-PMC1796906-4-pmedp0040039pg004.jpg"
} | 000434 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
|
{
"caption": "Survival and Migration of Human NSCs in Rat Spinal CordPhotomicrographs and graphs in (A–C) illustrate the localization and numbers of HNu (+) cells at different time points postgrafting, whereas (D) and (E) support the migratory phenotype of grafted HNu (+) cells, and (F) confirms their low mitotic activity.(A and B) At 3 mo postgrafting most HNu (+) cells, indicated as red profiles with an arrow in (A), are located around the injection sites and along needle tracks. By six months (B), HNu (+) cells show widespread migration away from the injection site in both the gray and white matter, and many are seen in the white matter and a few in the gray matter of the contralateral side. (B) is a composite of several fields to show the extent of migration. Arrow in (B) shows the colonization, by NSC-derived cells, of the central nervous system portion of the dorsal root (note the central nervous system–peripheral nervous system transition zone).(C) Bar graphs showing HNu (+) cell numbers at the time of grafting (0) and at three weeks (3w), three months (3m), and six months (6m) postgrafting in the different treatment groups (avulsion, red; HCA treatment, blue; sham, green). Far left graph shows numbers of HNu (+) cells ipsilateral to the grafting site (Ipsi), and far right graph shows numbers on the contralateral gray matter (Contra). Brackets show the results of post hoc testing when ANOVA was significant in the avulsion and HCA groups ipsilateral to grafting; in all other cases, significance was established with a Student's t-test. Asterisk indicates statistical significance at p ≤ 0.05. Method of section selection is illustrated on the extreme left.(D) Dcx, a marker for migrating neuronal precursors, was expressed by about 80% of grafted cells 3 wk postgrafting. Dcx expression is reduced to 10%–15% of HNu (+) cells surrounding the grafting sites at 3 and 6 mo but remains very high (~ 80%) in HNu (+) cells on the contralateral gray matter up to 6 mo postgrafting.(E) A confocal image of HNu (+) (red) cells also labeled with Dcx (green) at 3 wk postgrafting.(F) The three images illustrate, on a section that was dually stained for HNu (red nuclear marker on the left) and Ki67 (green nuclear marker in the center), the very low rate of mitotic activity (double-stained nuclei on the right) in NSC grafts. The single double-stained nuclear profile is indicated with an arrow.Scale bars: (A) 200 μm; (B) 600 μm; (E) 10 μm; (F) 20 μm.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_101-PMC1796906-7-pmedp0040039pg002.jpg"
} | 000435 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
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{
"caption": "Neurotransmitter Differentiation of Grafted Human NSCsPhotomicrographs (A–J) illustrate evidence of glutamatergic (A and B, G and H), GABAergic (C–F), and cholinergic (I and J) neurotransmission in NSC grafts. As in previous figures, confocal microscopy is used primarily to confirm the colocalization of two markers in the same cellular compartment along three planes of sectioning.(A and B) These sections, stained for HNu and the prevalent AMPA receptor epitope GluR2/3, show both cytoplasmic and synaptic staining by epifluorescence (A) or confocal (B) microscopy. Insets in (A) represent magnifications of indicated neurons in main image; top- and bottom-left insets show two medium-size HNu (+) cells with cytoplasmic immunoreactivity, whereas bottom-right inset illustrates a larger HNu (+) cell containing multiple GluR2/3 (+) boutons.(C and D) These sections are stained for HNu and the GABA-synthesizing enzyme GAD and visualized with epifluorescence (C) or confocal microscopy (D). Arrows in (C) indicate multiple HNu (+) cells with cytoplasmic GAD immunoreactivity.(E and F) Confocal microscopy of a field stained with both human Syn (red in single-channel image on top left, to label graft-derived terminals) and GAD (green in single-channel image on bottom left, to label GABAergic terminals) shows colocalization of the two proteins (yellow color in merged images in F) in multiple synaptic boutons. Nearly all graft-derived boutons are inhibitory (F).(G and H) These sections (G, epifluorescence; H, confocal) are stained for human Syn to label graft-derived terminals (red) and mixed VGLUT1/ VGLUT2 antibodies to label glutamatergic terminals in the field (green). Despite significant overlap and apposition of graft-derived and VGLUT1/2 (+) terminals (G), the two groups of terminals are separate (H).(I and J) These two sections were dually stained for: HNu and choline acetyltransferase (I and insert) epifluorescence; confocal microscopy (J); and show that some of the largest NSC-derived neurons express cholinergic phenotypes. These cells elaborate multiple primary dendrites (I and insert). (J) is the confocal image of the neuron in the inset.Scale bars: (A), (C), (G), (I) 20 μm; (B), (D–F), (H), (J) 10 μm.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_101-PMC1796906-8-pmedp0040039pg005.jpg"
} | 000436 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
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{
"caption": "Spinal Cord Progenitors Isolated from the Cord and Grown in Tissue Culture as Adherent Cultures or Nonadherent NanospheresMore heterogeneity is seen after adherent culture compared to neurosphere culture.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_101-PMC1796907-0-pmedp0040048pg002.jpg"
} | 000437 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
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{
"caption": "Immunofluorescent Characterization of the Neoplastic Stromal Cells in Hemangioblastoma Tissue Sections(A) Neoplastic cells express the mesodermal marker, brachyury (green), Flk-1 (VEGF receptor-2; red), and the nuclear stain 4′,6-diamidino-2-phenylindole (DAPI) (blue). Coexpression of brachyury and Flk-1 (VEGF receptor-2) indicates that these cells are embryologic hemangioblasts.(B) Expression of Scl (red) by the neoplastic cells indicates that these are hematopoietic stem cells committed to hematopoietic and endothelial lineages.(C) The neoplastic cells demonstrate scattered cytoplasmic expression of the hematopoietic stem cell antigen CD133 (red).(D) Coexpression of the erythropoietin (green) and erythropoietin receptor (red) was uniformly demonstrated on the neoplastic cells.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_101-PMC1796910-1-pmedp0040060pg002.jpg"
} | 000438 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
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{
"caption": "Hematopoietic Progenies Are Derived from Hemangioblastoma Tumor Cells(A) Giemsa staining of the vacuolated neoplastic stromal cell of the hemangioblastoma.(B) Giemsa staining of early (arrows), late (arrowheads), and dividing (open arrow) nucleated erythrocytes expanding in an erythropoietin-enriched medium.(C) Giemsa staining of immature (arrow) and mature (arrowhead) polynuclear granulocytes expanding in an erythropoietin-enriched medium.(D) While loss of heterozygosity was not detected in peripheral blood mononuclear cells (PB), deletion of the wild-type VHL allele occurred in cultured hemangioblastoma (T) and cultured nucleated erythrocytes (NE), confirming the hemangioblastic origin of the nucleated erythroid cells.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_101-PMC1796910-3-pmedp0040060pg004.jpg"
} | 000439 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
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{
"caption": "Distribution of Hemangioblastomas in the Central Nervous Systems of Study Patients(A) Schematic representation of the distribution of CNS hemangioblastomas (red dots) in the 25 von Hippel-Lindau disease patients on MRI. Most (98%) of hemangioblastomas were found below the level of the tentorium in the cerebellum, brainstem, and spinal cord.(B–D) Contrast-enhanced MRI demonstrating representative locations of hemangioblastomas including the cerebellum (B), brainstem (C) and spinal cord (D). (B) Axial view through the cerebellum demonstrating a hyperintense enhancing hemangioblastoma (arrow) with surrounding edema (hypointense area surrounding the tumor) that frequently is associated with these lesions. (C) Sagittal view through the posterior fossa demonstrating a hyperintense enhancing brainstem (medullary) hemangioblastoma (arrow) with surrounding edema. (D) Sagittal view through the thoracic and lumbar spinal cord demonstrating two hyperintense enhancing hemangioblastomas (arrows). The superior tumor is associated with a large intraspinal cyst (syrinx) that is common with these neoplasms (arrowhead).",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_101-PMC1796910-4-pmedp0040060pg001.jpg"
} | 000440 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
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{
"caption": "Fluorescently labeled Versazyme™ infusing into MBM particles. Top row (left to right) is a soft tissue particle 0, 20, 90 and 300 minutes after exposure to the Versazyme™ solution. Bottom row is a bone particle 0, 10, 30 and 60 minutes after exposure. White bar is 25 µm.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_101-PMC1796944-1-ponep0000245pg002.jpg"
} | 000441 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
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{
"caption": "Penetration of fluorescently labeled Versazyme™ (represented by red color) into a bone particle, after 10 minutes incubation. Arrow indicates a fissure in the particle. White bar is 25 µm.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_101-PMC1796944-2-ponep0000245pg003.jpg"
} | 000442 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
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{
"caption": "Representative MBM particles autofluorescing. Particles in top row are soft tissue, particles in bottom row are bone. Arrows indicate ‘fissures’; white bar is 25 µm.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_101-PMC1796944-6-ponep0000245pg001.jpg"
} | 000443 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
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{
"caption": "Angio (A) and noninvasive coronary angiography using MSCT (B) and MRI (C) all demonstrate absence of significant stenoses in the right coronary artery (arrow) in a 45-year-old female patient with atypical angina pectoris. Note that MSCT due to higher spatial resolution allows better delineation of the distal segments of the right coronary artery than MRI (asterisks).",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_101-PMC1796945-6-ponep0000246pg004.jpg"
} | 000444 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
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{
"caption": "Carotid 2D and TDI imaging. Digitized longitudinal 2D scan of the common carotid artery (CCA) just prior to the bifurcation (right) and optimized ROI window with color tissue Doppler of the CCA.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_101-PMC1797002-7-1476-7120-5-6-3.jpg"
} | 000445 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
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{
"caption": "eGFP staining in representative lung panels. Representative panel of a control mouse lung (A and B) constitutively expressing GFP and wild-type mouse lung (C) injected with eGFP bone marrow-derived cells (BMDCs). Panels D, E, F and G are representative panels of recipient mouse lung 12 days after injection with eGFP BMDCs, which occurred 3 days after acute lung injury by a single administration of MCTp. Staining was detected in few cells in alveolar space, bronchial and distal arteries. Panel H. representative panel of recipient mouse lung exposed to hypoxia for 15 days and 12 days after injection with eGFP BMDCs: no staining was detected. Original magnification ×125 (A, D and H) ×500 (B, C, E, F and G).",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_101-PMC1797016-1-1465-9921-8-8-6.jpg"
} | 000446 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
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{
"caption": "In vivo inhibition of VM with treatment and presence of VM in breast cancer specimens. (a) Vascularity of tumor xenografts in mice was evaluated by factor VIII related antigen staining (brown) for endothelial cells, and Biebrich Scarlet staining (red) for RBCs. (b) Absence of endothelial cells lining the pools of RBCs (vascular channels) was shown by no staining for factor VIII related antigen and positive staining for RBC by Biebrich Scarlet. C) Histological features of the primary human grade 1 breast tumor specimen showing blood vessels positive for CD34 staining (brown) for endothelial cells, and Biebrich Scarlet staining (red) for RBCs. No vascular channels were detected. (d) RBC pooling without the lining of endothelium (vascular channels) in the high-grade invasive ductal breast carcinoma specimen. (e, f) COX-2 staining pattern in grade 1 primary human breast tumor specimen (panel e) and in high-grade invasive ductal carcinoma specimen (penal f). Magnifications are as follows: panels a and b, 100 ×; panels c and d, 200 ×; and panels E and F, 100 × magnification). COX, cyclo-oxygenase; RBC, red blood cell; VM, vasculogenic mimicry.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_101-PMC1797025-0-bcr1626-6.jpg"
} | 000447 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
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{
"caption": "Kinetics of vascular channel formation by breast cancer cells. MDA-MB-231, MDA-MB-435, MCF-7, and ZR-75-1 cells were serum starved overnight, plated on matrigel, and images using phase contrast microscopy were taken at the times indicated. The MDA-MB-231 and MDA-MB-435 cells started to form vascular channels as early as 2 hours after plating on matrigel. Well defined patterned networks were observed by 7 hours. MCF-7 and ZR-75-1 cells failed to form patterned networks.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_101-PMC1797025-1-bcr1626-1.jpg"
} | 000448 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
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{
"caption": "COX-2 inhibition by celecoxib or specific siRNA inhibits vascular channel formation. (a) Phase contrast images show vascular channel formation in growth factor reduced matrigel of MDA-MB-231, treated with vehicle or 40 mmol/l celecoxib. Images were captured 48 hours after plating using a phase contrast microscope. (part i) With vehicle treatment, MDA-MB-231 cells form well differentiated tubular structures. (part ii) With celecoxib treatment, differentiation into channels was significantly reduced in MDA-MB-231 cells. (part iii) Addition of 50 ng/ml PGE2 to MDA-MB-231 cells treated with 40 μM celecoxib could reverse the inhibitory effect of celecoxib. (b) COX-2 expression decreases in MDA-MB-231 cells with siRNA treatment. COX-2 protein expression was measured by Western blot. Treatment with a COX-2 siRNA for 48 hours significantly inhibited COX-2 expression at siRNA concentrations of 10, 50, and 100 nmol/l. Data shown are representative of three independent experiments. (c) Inhibition of vascular channel formation in MDA-MB-231 cells with celecoxib and COX-2-specific siRNA treatment. Quantitative analysis of vascular channel formation: the number of vascular channels was determined by counting the number of connected cells in five randomly selected fields, using 200 × magnification, and dividing that number by the total number of cells in the same field. Raw data from five standardized fields for each treatment from three separate experiments are shown. Treatment with 40 and 60 mmol/l celecoxib and treatment with a 50 nmol/l concentration of COX-2 siRNA for 48 hours caused significant decrease in the number of channels formed by MDA-MB-231 cells, and addition of 50 ng/ml of PGE2 was able to reverse the effect observed with treatment with 40 mmol/l celecoxib. P values represent significant difference between vehicle control and celecoxib treatment. COX, cyclo-oxygenase; PG, prostaglandin; siRNA, small interfering RNA.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_101-PMC1797025-3-bcr1626-3.jpg"
} | 000449 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
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{
"caption": "Cross-priming of cyclin B1 specific CTLs.(a) Fluorescence microscopy analysis of cyclin B1 staining with T47D breast cancer cells. No staining with isotype control could be seen. (b) CTLs primed as described in Figure 3 killed T2 cells pulsed with cyclin B1 derived CB9 peptide, but not T2 cells pulsed with irrelevant (PSA) peptide, indicating the presence of cyclin B1-specific CTLs. Shown are representatives of three experiments. Values are expressed as average and standard deviation of triplicate wells. CTL, cytotoxic T lymphocyte; DC, dendritic cell.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_101-PMC1797030-2-bcr1621-4.jpg"
} | 000450 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
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{
"caption": "MRI of bilateral elastofibroma with the tumour being located between the thoracic wall, the anterior serratus, and the latissimus dorsi muscle (coronal (2a) and axial (2c) T1-weighted images): The small arrows indicate the medial margins of the lesions containing fatty (bright) and fibrous (dark) tissue. The tumours are located between the thoracic wall, the anterior serratus, and the latissimus dorsi muscle. The large arrows points to the margo inferior of the scapula. Figures [2b] and [2d] show the corresponding STIR -sequences with a slightly inhomogenous signal intensity within the elastofibromas.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_101-PMC1797045-1-1477-7819-5-15-2.jpg"
} | 000451 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
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{
"caption": "Microscopic findings in elastofibroma dorsi: 4a): Fibrous, collagenous strands intermingled with fat cells (hematoxylin-eosine-staining). 4b): Collagenous material and roundly shaped elastic fibres, mesenchymal cells with bland nuclei (hematoxylin-eosine-staining). 4c): Elastic fibres and structures forming discs and globules stained dark brown to black using an elastic stain (Elastica-van-Gieson).",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_101-PMC1797045-3-1477-7819-5-15-4.jpg"
} | 000452 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
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{
"caption": "Representative photomicrographs of immunohistochemistry for tyrosine hydroxylase (TH) labeled neurons in AVPV of 150 day-old Long-Evans rats fed either an isoflavone-free diet (Phyto-free) or an isoflavone-rich diet (Phyto-600) from conception until time tissue collected. TH-immunoreactive(ir) cells were visualized with diaminobenzadine (DAB) and seen as brown cytoplasma staining. Sections were counterstained with hematoxylin (blue). Positive control of TH staining is shown in Phyto-free fed female AVPV (A). In males, no significant differences in the number of TH-ir neurons were observed between Phyto-600 (C) and Phyto-free AVPV (B). The TH-ir cells in the boxed regions were indicated (*) at high magnification in the up-left corner of B and C. Bar = 25 μm for all the photomicrographs.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_101-PMC1797051-0-1471-2202-8-13-4.jpg"
} | 000453 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
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{
"caption": "Representative photomicrographs of apoptotic cells (brown nuclear staining), astrocytes (red) and neurons (green) by TUNEL and dual GFAP/NeuN immunofluorescent staining in AVPV of 150 day-old male Long-Evans rats. All the photomicrographs (coronal brain sections) are from animals fed an isoflavone-rich diet (Phyto-600) from conception until time tissue was collected. An astrocyte (red; arrow) is indicated in A, whereas the neurons are displayed in green. B is a representative photomicrograph of TUNEL staining in AVPV (n = 5). C displays dual GFAP/NeuN immunofluoresent stained sections within AVPV, which is 6 μm apart from B. The apoptotic cells (arrows in B) were identified as neurons (arrows in C). Bar = 25 μm for all the photomicrographs.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_101-PMC1797051-1-1471-2202-8-13-3.jpg"
} | 000454 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
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{
"caption": "Representative photomicrographs of apoptotic cells (TUNEL) and ERalpha-immunoreactive cells (ERalpha IHC) in AVPV of 150 day-old male Long-Evans rats. All the photomicrographs (coronal brain sections) are from animals fed an isoflavone-rich diet (Phyto-600) from conception until time tissue collected. ERalpha-immunoreactive (ir) cells were visualized with diaminobenzadine (DAB) and seen as brown nuclear staining (arrows in ERalpha). Sections were counterstained with hematoxylin (blue). A, C, E, G and I are representative photomicrographs of TUNEL staining in AVPV (n = 5). B, D, F, H and J are ERalpha IHC stained sections within AVPV, which are 6 μm apart from A, C, E, G, and I, respectively. Apoptotic cells (stars in TUNEL) were not ERalpha-ir (stars in ERalpha). Bar = 25 μm for all the photomicrographs.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_101-PMC1797051-3-1471-2202-8-13-5.jpg"
} | 000455 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
|
{
"caption": "Representative photomicrographs of apoptotic cells labeled by TUNEL staining in AVPV of 150 day-old male Long-Evans rats fed either an isoflavone-free diet (Phyto-free) or an isoflavone-rich diet (Phyto-600) from conception until time tissue collected. Apoptotic cells were visualized with diaminobenzadine (DAB) and seen as brown nuclear staining. Sections were counterstained with hematoxylin (blue). A and C are representative photomicrographs of the Phyto-free AVPV (n = 5). B and D are representative photomicrographs of the Phyto-600 AVPV (n = 5). Across the diet treatments, A corresponds to a similar coronal brain section in B. The AVPV is outlined with dashed lines. Boxed regions in A and B are magnified and shown in C and D, respectively. Significantly more apoptotic cells (arrows) were observed in Phyto-600 AVPV than the Phyto-free group (n = 5; p < 0.001; two-sample student t-test). Bar = 25 μm for all the photomicrographs.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_101-PMC1797051-5-1471-2202-8-13-1.jpg"
} | 000456 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
|
{
"caption": "Representative photomicrographs of ERalpha and ERbeta-immunoreactive cells (IHC staining) in AVPV of 150 day-old male Long-Evans rats. All the photomicrographs (coronal brain sections) are from animals fed an isoflavone-free diet (Phyto-free) from conception until time tissue collected. ER-immunoreactive (ir) cells were visualized with diaminobenzadine (DAB) and seen as brown nuclear staining. Sections were counterstained with hematoxylin (blue). A, C, E and G are representative photomicrographs of ERalpha IHC staining in AVPV (n = 2). B, D, F and H are ERbeta IHC stained sections within AVPV, which are 6 μm apart from A, C, E and G, respectively. Some cells are both ERalpha- and ERbeta-ir (stars for ERalpha and ERbeta). The number of cells in the AVPV that express both ERalpha and ERbeta is approximately 20–22% of the total number of cells within this nuclear structure. Some cells are only ERalpha-ir or ERbeta-ir (arrows in ERalpha or ERbeta). Bar = 25 μm for all the photomicrographs.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_101-PMC1797051-6-1471-2202-8-13-8.jpg"
} | 000457 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
|
{
"caption": "A) Mild staining of epidermal keratinocytes and lack of staining in dermal cells in non lesional skin biopsy. B) Strong cytoplasmic staining of epidermal keratinocytes, dermal endothelial and inflammatory cells. IL-8 IHC, DAB, Hx counterstaining × 40",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_101-PMC1797156-0-1746-1596-2-4-1.jpg"
} | 000458 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
|
{
"caption": "Stretched, i.e. elongation of sarcomeres and nuclei in viable myocardial cells in an old aneurysm of the left ventricular wall with dense fibrosis [A]. Dense and compact fibrosis as end result of a repair process of an infarct. The collagen fibers are straight and closed together [B]. In contrast in hearts with congestive heart failure the myocardial fibrosis is very mild [C, D] showing a corkscrew aspect of the collagen fibers [E]. This means an adaptation of the collagenogenesis to the contraction cycle without any capability to reduce or stop the latter. Furthermore, the fibrous tissue may metaplasize in adipose tissue which substitutes large fibrous area [F, G]. A fact to have in mind when quantifying the size of a scar or measuring by nuclear method the myocardial viability.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_101-PMC1797157-0-1476-7120-5-5-1.jpg"
} | 000459 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
|
{
"caption": "Weight/size paradox in congestive heart failure. The slippage of myocardial cell to explain a normal wall and myocellular size despite a heavy cardiac weight [A], does not consider the myofibrillar bridging between myocells and fibrillar collagen connections. Slippage of myocells, capable to reduce for instance a 3 cm cardiac wall in 1.5 one, should imply an extensive destruction of all interstitial structures [vessels, including lymphatics, and nerves] and consequent widespread tissue damage never seen in this condition. Even a neogenesis of the myocardial cell was never observed. Only once in an endomyocardial biopsy at a previous site of sampling in a transplanted heart, we had the opportunity to see new myocells forming a focus of small elements assuming the aspect of atrio-ventricular node [B, C]. Just to emphasize that a neogenesis when exists can be seen, apparently without integration in the functioning myocardium.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_101-PMC1797157-1-1476-7120-5-5-2.jpg"
} | 000460 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
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{
"caption": "Colliquative myocytolysis. Progressive disappearance of myofibrils [A], with intramyocardial edema [B] resulting in empty sarcolemmal tube seen in longitudinal [C] and transverse section [D]. Note the absence of any type of reaction. E, ultrastructural view of an edematous myocell filled by mitochondria and few contracted myofibrils in contrast with a normal contracted myocell [F] of the same case with congestive heart failure.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_101-PMC1797157-3-1476-7120-5-5-3.jpg"
} | 000461 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
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{
"caption": "Islets from Idd9 congenic mice are intrinsically resistant to cytokine and CD8 T cell-mediated autoimmune destruction. Islets isolated from 5 week old Idd9 congenic and NOD donors were transplanted under the kidney capsule of NODScid recipients. After allowing 7 days for the grafts to revascularize, splenocytes from 8.3NODScid mice (30 million cells) were adoptively transferred into graft recipients. Control mice did not receive splenocytes. Five days later, graft destruction was analyzed by H&E staining of graft sections, as shown in (A) (i-iv). Scoring of graft destruction (B) was determined by anti-glucagon staining (A) (v-vi) as described in the methods. NOD grafts (n = 4), NOD control grafts (n = 2), Idd9 congenic grafts (n = 3) and Idd9 control grafts (n = 2) were assessed, and a total of 24, 11, 26 and 32 islets scored, respectively, for each group. Graph shows average % healthy islets in each graft +/- SEM. Results representative of 3 independent experiments. (C) Confocal live/dead viability staining of islets from 5 week old Idd9 congenic and NOD mice treated with, or without, TNF (2,000 U/ml) and IFNγ (1,000 U/ml) for 6 days. Live cells stain green, and the nuclei of dead cells stain red. For each assay, 7–9 islets (250–500 cells) were analyzed and the average % dead cells per field +/- SEM is shown in (D). Results representative of 3 independent experiments.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_101-PMC1797159-0-1745-6150-2-5-4.jpg"
} | 000462 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
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{
"caption": "Detection of TNFR2 expression in islet cells. (A) Flow cytometry staining with anti-TNFR2 antibody and isotype control in islets isolated from 5 week old mice and cultured for 4–5 days. Auto-fluorescence in the FL1 channel was used to gate on the β cell population, and dead cells were excluded using 7AAD. The gated cells were negative for the hematopoetic cell marker CD45. Values given are the average %TNFR2+ cells +/- SEM (n = 4 each strain), gated on FL1+ cells and corrected for background isotype control staining. Results representative of 3 independent experiments. (B) Immunohistochemical staining of paraffin sections from TNFR2KO (i, iv), 12–14 week old NOD (ii, v) and Idd9 congenic (iii, vi) pancreas stained with antibodies to TNFR2 (i-iii) or with the secondary antibody alone (iv-vi). Pancreatic sections from 3 different Idd9 congenic and NOD mice, and 1 B6.TNFR2KO mouse, were examined.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_101-PMC1797159-3-1745-6150-2-5-5.jpg"
} | 000463 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
|
{
"caption": "Co-localization of TRAF2 and TNFR2 following cytokine treatment is prolonged in NOD β cells. Expression of the adaptor molecules TRAF2 and RIP in islets. (A) Immunohistochemical staining of pancreas sections from 12–14 week old NOD and Idd9 congenic mice with anti-TRAF2 antibody. Immunoblotting with antibodies to TRAF2 (B) and RIP (C) of protein lysates prepared from 5 week old Idd9 congenic (I) and NOD (N) islets stimulated for 3 days +/- 1,000 U/ml TNF + 1,000 U/ml IFNγ. In each case, membranes were stripped and re-blotted with antibodies to actin as loading control. Results representative of 2–3 independent experiments. (D) Co-localization of TRAF2 and TNFR2 following cytokine treatment determined by confocal imaging. Representative images of β cells from 5 week old NOD and Idd9 congenic mice treated with 1,000 U/ml IFNg + 1,000 U/ml TNF for 0, 30 and 60 minutes, stained with antibodies to TNFR2, TRAF2 and insulin, and the nuclear dye DAPI. The merged image of TNFR2 (green), TRAF2 (red) and DAPI (blue) is also shown. Dead cells were excluded using an EtBr-derived dye. In (E) the average TNFR2/TRAF2 colocalization coefficient (M1) +/- SEM is shown for each time point. TNFR2 positive cells are rare within the islet population, but on average 18 TNFR2 positive cells were analyzed per treatment. The threshold for TNFR2 and TRAF2 staining was determined by staining an aliquot of the cells in parallel minus each primary antibody.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_101-PMC1797159-6-1745-6150-2-5-7.jpg"
} | 000464 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
|
{
"caption": "Radiograph taken after consolidation phase showing the formation of new bone at the distraction site.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_101-PMC1797165-10-1746-160X-3-7-7.jpg"
} | 000465 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
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{
"caption": "Panoramic radiograph taken at the beginning of distraction showing the osteomies.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_101-PMC1797165-8-1746-160X-3-7-6.jpg"
} | 000466 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
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{
"caption": "Contrast enhanced CT scan shows a soft tissue mass, 16 × 17 mm in size in the lateral part of the right external auditory canal posterior wall. Diagnosis was reported to be schwannoma by histologic examination.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_101-PMC1797166-0-1746-160X-3-6-1.jpg"
} | 000467 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
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{
"caption": "Histopathologic section of the tumor demonstrating areas of compact spindle cells arrayed in a palisade pattern called as Antony A. (H&E staining, original magnification ×10)",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_101-PMC1797166-1-1746-160X-3-6-2.jpg"
} | 000468 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
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{
"caption": "Benchmark objects used: Left: Photo of a sheep's skull containing small intrinsic bone surface features and tool marks, including add-ons such as boreholes, countersink drillings, red and black felt pen marks. Right: Photo of a sandstone conglomerate featuring different inclusions, some of dark light absorbing quality, some highly reflective.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_101-PMC1797168-1-1471-2342-7-1-1.jpg"
} | 000469 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
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{
"caption": "Experimental subjective evaluation was conducted using a set of 20 comparisons just as the three visual objects (a, b, c) displayed here, evaluated by 12 participants. For each of the rows, scanner 'x' and scanner 'y' were presented in random sequence to participants who had to select the preferred match ('x' or 'y') as a forced choice. In this illustration, 'x' is QTSculptor, 'y' is ATOS-II for all three visual objects (a,b,c). a: Bone surface structure featuring finely granular roughness, and sharp edges (bottom of foramen), that blend into the surface (lateral margins of the foramen). b: Bone surface structure featuring finely granular roughness, a black felt pen dot, several small surface indents, and a suture. c: Bone surface containing a more extensive suture containing countersink drillings. Bar is 5 mm.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_101-PMC1797168-2-1471-2342-7-1-6.jpg"
} | 000470 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
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{
"caption": "Quantitative characterization of scanner performance was done using a set of 220 single surface areas, 3 of which are presented for illustration with photos of real object (a,d,g), ATOS-II-derived data (b,e,h) and QTSculptor-derived data (c,f,i). Red outlines mark homologue areas. Bar is 5 mm.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_101-PMC1797168-3-1471-2342-7-1-7.jpg"
} | 000471 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
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{
"caption": "Side-by-side comparison of real forensic skin pathology (a, b, d, e, g, h) and benchmark object features (c, f, i): Deep facial abrasion after sliding over a rough surface (a, b) containing various highly reflective surface regions, patchy dark discoloration as well as bumpy appearance. These surface shape elements are represented on a similar scale on the rock surface that we used as benchmark object (c). Superficial abrasions as found in gunshot entry wounds (d, e: arrow) are also present on skull surface (f: arrow) used as benchmark object. Curved (g: arrow) and straight (h: arrow) wound edges as found in stabs from a serrated (g) or straight (h) knife blade are represented by a bony suture of the skull (i: arrow) used as benchmark object (bar 1 cm).",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_101-PMC1797168-4-1471-2342-7-1-3.jpg"
} | 000472 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
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{
"caption": "Sheep skull benchmark object. a): Photo. b) Matching the photo, this shows the 3D model obtained using ATOS-II 3D scanner. c) Matching the photo, this shows the 3D model obtained using QTSculptor scanner. Details of the scratch contained on the surface were reproduced photographically (d), on the ATOS-II scanner (e) and QTSculptor (f). See text for feature description. Bar is 5 mm.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_101-PMC1797168-5-1471-2342-7-1-4.jpg"
} | 000473 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
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{
"caption": "Rock benchmark object. a): Photo. b) Matching the photo, this shows the 3D model obtained using ATOS-II 3D scanner. c) Matching the photo, this shows the 3D model obtained using QTSculptor scanner. A detailed region of this surface was reproduced photographically (d), on the ATOS-II scanner (e) and QTSculptor (f). See text for feature description. Bar is 5 mm.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_101-PMC1797168-6-1471-2342-7-1-5.jpg"
} | 000474 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
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{
"caption": "Eye phenotypes induced by secreted anti-Pax6 single-chain antibody. (a-f) Eye phenotypes observed at 30 hpf after injection of secreted single-chain anti-Pax6 (spaP6) mRNA into one cell embryos. (a) Normal, (b) small eyes, (c) dissymmetric eyes, (d) single eye, (e) no eye, (f) cyclops. (g) Quantification (percentage) of phenotypes: n, normal; d, dissymmetric, reduced and absent eyes (pooled); c, cyclops. Comparison between secreted anti-Pax6 (spaP6, n = 221, red), secreted anti-En (spaEn, n = 251, blue) and non-secreted anti-Pax6 (aP6, n = 178, dark red). Differences between spaP6 and spaEn or aP6, as well as between aP6 and spaEn, are statistically significant (p < 0.001, χ2 tests). (h) Quantification of single-chain mRNA expression. Top: western blot of protein extracts from embryos injected with the indicated RNAs (no : no RNA); the arrow indicates a single-chain antibody signal at approximately 34 kDa, and the arrowhead the cross-hybridizing protein species used for calibration. Bottom: densitometric scans of western signals indicate aP6 expression (ratio 1:1 with the endogenous signal) is at least as high as spaP6 expression (ratio 0.7:1 with the endogenous signal).",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_101-PMC1797170-1-1749-8104-2-2-2.jpg"
} | 000475 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
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{
"caption": "Eye phenotypes induced by intercellular injection of anti-Pax6 monoclonal antibody. (a-d) Intercellular distribution of FITC-labeled anti-mouse antibody injected at the blastula stage. Embryos were analyzed in toto (a,c) or using confocal microscopy (b,d) either 1 hour after injection (a,b) or at the shield stage (c,d). Note that the staining is concentrated in the intercellular space and is virtually absent from the cell interior. (e-m) Prototypical eye phenotypes (quantifications in Figure 4): Embryo 1 (e-g) has a severely reduced right eye (lateral view (e)) compared with left eye (lateral view (f)) and no right retina or lens (section in (g)). Embryo 2 (h-j) has a strongly reduced left eye (frontal view in (h), lateral view in (i)). The horizontal section (j) confirms the phenotypes and indicates that the left lens is also reduced (arrow). Embryo 3 has two reduced eyes with disorganized morphologies (lateral views in (k,l)) and a reduced and misplaced left lens (red arrow in (l,m)). In all horizontal sections, rostral is to the left.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_101-PMC1797170-2-1749-8104-2-2-3.jpg"
} | 000476 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
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{
"caption": "Examples of eosin-hematoxylin stained tumor-tissue of the central (A) and peripheral (B) part of the mammary tumor in control (left) and during hyperoxic treatment (right, 1 bar, pO2 = 1.0). The images under A are scaled to the same magnification (× 4) and the images under B to the same magnification (× 10). Scale bar indicate 500 μm (A) and 100 μm (B).",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_102-PMC1797183-0-1471-2407-7-23-2.jpg"
} | 000477 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
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{
"caption": "Bax mitochondrial translocation after Ad-E2F-1 expression in SK-MEL-2 cells and HCT116 PUMA+/+ cells. SK-MEL-2 cells (a,b) and HCT116PUMA+/+ cells (c,d) were infected with Ad-LacZ and Ad-E2F-1. After 24 hours of infection, cells were stained with Mitotracker Red 580 and Bax (green), then counterstained with DAPI (blue) as described in the Methods. After 24 hours of Ad-E2F-1 infection in SK-MEL-2 cells (b) and HCT116PUMA+/+ cells (d), Bax clustered and translocated to mitochondria (an overlay of the three stains is given in yellow, which shows co-localization of Bax in mitochondria).",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_102-PMC1797184-0-1471-2407-7-24-5.jpg"
} | 000478 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
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{
"caption": "Specific expression of reporter by the Rev-dependent lentiviral vector in HIV-infected primary macrophages. M-CSF-treated monocyte-derived macrophages were infected or were not infected with HIVAD8, followed by Rev-dependent reporter virus vNL-GFP-RRE(SA). (A). Bright-field image of fixed cells with black bar representing 50 micrometers. Nuclei are prominent. Cells that were found to stain positive for HIV p24 are numbered in this image. B. Red fluorescence of identical field. All cells were stained for anti-p24 antibody, followed by a secondary red fluorescent antibody to detect productively infected cells. Intracellular red fluorescent foci identify productive infection; nine cells were identified in this field (B). We examined macrophage populations with four concentrations of HIV input (36, 72, 169, and 361 ng p24 HIVAD8/106 cells) followed by a constant input of reporter lentiviral vector (5 ng p24 vNL-GFP-RRE(SA)/106 cells; VSV-G envelope). Independent of the input of HIV, approximately one fifth of the p24-positive cells expressed GFP (23.0 ± 2.8%; mean ± SD; n = 4), as shown by green fluorescence in this same field (C). Magnification was identical on all three images. The reporter virus was demonstrated to function in macrophages from three donors.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_102-PMC1797186-2-1742-4690-4-12-3.jpg"
} | 000479 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
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{
"caption": "Prolonged exposure to thymidine results in cellular senescence. (A) Following a 1 mM thymidine 24 hour pre-treatment, cells were electroporated and released into complete medium (1-0 mM) or medium supplemented with 2 mM thymidine (1–2 mM). At 48, 72 and 144 hours, cells were stained for the senescent marker β-galactosidase. The four quadrants for the 1–2 mM sample at 48 hours are combined images obtained from four different fields of view, due to the low confluency of the sample. (B) Images depict varying views and different wells of the 1–2 mM thymidine treated cells at 144 hours. All images are at 20× magnification and counts of individual cells in a representative field were performed to evaluate the degree of senescence induction.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_102-PMC1797188-2-1471-2199-8-9-5.jpg"
} | 000480 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
|
{
"caption": "In situ expression pattern of germ cell-related markers (MAGE-A4 and TSPY) in OGCTs and sex centromere material in interphase nuclei from OGCTs. MAGE-A4 in A1: Dysgerminoma and A2: In foetal ovary of GW 28 with strong expression in oogonia (lower left), and no expression in developing follicles (top right); TSPY in B1: Gonadoblastoma (Case PT-04) and B2: Dysgerminoma (Case PT-57). Scalebar = 25μm. Fluorescence in situ hybridisation of two different dysgerminomas with sex chromosome centromeres: C. Presence of X and Y chromosome material (Case PT-04) and D. Presence only of X chromosome material (Case PT-14). E-F: Inserts are control DAPI-only.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_102-PMC1797189-1-1476-4598-6-12-2.jpg"
} | 000481 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
|
{
"caption": "Expression of stem cell related markers and KIT mutations in dygerminomas. A. Immunohistochemical staining for OCT-3/4, KIT, NANOG, and AP-2γ in dysgerminomas. Scale bar = 25 μm. B. Examples of KIT mutation analysis, with control sequence from normal blood, and three of the mutated KIT (codon 816) sequences from dysgerminomas.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_102-PMC1797189-2-1476-4598-6-12-1.jpg"
} | 000482 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
|
{
"caption": "Processed bark (cortex) from the evergreen tree Eucommia ulmoides. Originating from temperate regions of China, botanical parts of E. ulmoides such as the leaves and bark are used medicinally in the Chinese and Japanese Pharmacopia. Note the silvery threads of resin (Gutta Percha) in between the sliced portion of the specimen (green arrow).",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_102-PMC1797194-4-1472-6882-7-3-1.jpg"
} | 000483 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
|
{
"caption": "Translocation of N. meningitidis to the respiratory mucosa is dependent on PilC1. (A) Bioluminescent imaging of mice and nasal washes spread on GC-plates 24 h post infection. CD46 transgenic mice were infected i.v. with wild-type FAM20LU or the PilC1-deficient mutant FAM20ΔpilC1\nLU. Nontransgenic mice were challenged with FAM20LU. Bacterial counts in the nasal washes (CFU/ml) are presented. Significantly fewer PilC1 mutants were recovered compared with wild-type bacteria (P<0.05). (B) Immunohistochemical detection of N. meningitidis in nasal cavity sections of CD46 transgenic mice at 24 h post-infection. Bacteria (dark brown) were detected with rabbit anti-N. meningitidis antibody followed by HRP-conjugated goat anti-rabbit IgG secondary antibody, and stained with DAB. Control sections have been treated without the first antibody (w/o 1st Ab). Scale bar: 20 µm.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_102-PMC1797199-4-ponep0000241pg005.jpg"
} | 000484 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
|
{
"caption": "N. meningitidis targets the thyroid gland and the nasal area of CD46 transgenic mice. (A) BLI of mice at 24 h after i.v. infection. Distinct bioluminescent signals are visible in the thyroid area (T) and oral-nasal region (N) of CD46 transgenic mice. (B) Ex vivo BLI imaging of a dissected thyroid. (C) Bacterial loads in blood and thyroid at 24 h post-infection. Significant difference between thyroid of CD46 transgenic mice and nontransgenic mice is indicated (P<0.001). Bars indicate mean ± SD. (D) Immuno-histochemical examination of thyroid gland tissue sections of mice infected for 24 h. Tissue sections were overlaid with antiserum against N. meningitidis, followed by a FITC-conjugated anti-rabbit IgG. Cell nuclei were counter-stained with DAPI (blue). Bacteria (shown in green) are detected in the cellular layer of thyroid follicles of CD46 transgenic mice (inset). Scale bar: 20 µm. (E) Thyroxine (T4) levels in mice. Sera were collected from CD46 transgenic (n = 6) and nontransgenic mice (n = 6) at indicated time points post-infection and T4 levels were determined by enzyme immunoassay (EIA). Uninfected CD46 transgenic mice had significantly less T4 than nontransgenic mice (P<0.05). Significant reductions of T4 compared with uninfected mice are indicated with asterisks (*, P<0.05).",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_102-PMC1797199-5-ponep0000241pg004.jpg"
} | 000485 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
|
{
"caption": "Taxol stabilized MT seeds strongly promote MT production and aster assembly in meiotic Xenopus egg extract. The two time series shown here were prepared as in Figure 1A but with addition of soluble Taxol (top series) or Taxol stabilized MT seeds (bottom series). Without MT seeds, the first traces of MTs were visible after ∼240 sec and organized asters (of 10–30 µm diameter) were visible after ∼480 sec. With MT seeds, organized asters were visible already after ∼240 sec. Thus, MT seeds speed up MT production by ∼240 sec. This supports a model where MTs induce the production of more MTs and that this mechanism is responsible for the assembly of MT super-structures.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_102-PMC1797610-0-ponep0000244pg002.jpg"
} | 000486 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
|
{
"caption": "During RanQ69L-mediated aster formation, MTs appear to accumulate exponentially at an initial seed MT. A. Quantification of RanQ69L-mediated MT production in meiotic Xenopus egg extract. RanQ69L was added to Xenopus extract to initiate the formation of spindle-like structures (asters). After fixation, asters were imaged by wide field microscopy at the indicated time points. The first detectable intermediates of aster formation were few individual MT bundles. Rapidly, more MTs were produced around these seed MTs. The diameter of the asters at 480 sec was 10–30 µm. B. Quantification of MT production mediated by RanQ69L. The plot shows fluorescence of the MTs at the prospective aster (with standard deviations) as a function of time. A logistic model can reproduce the initial exponential rise as well as the saturation of MT mass during aster formation. The logistic model with solution was fit to the experimental data of Figure 1 by minimizing A logistic model supports autocatalytic MT production where MT production is limited by resource depletion.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_102-PMC1797610-11-ponep0000244pg001.jpg"
} | 000487 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
|
{
"caption": "The actual points used in the analysis, selected at random within boundaries defined in the methods to conform with the specified isopleth rules, are plotted here in the upper row for data sets A, B, and C. For each set, the 20% isopleth surrounds the densest aggregation of points that appear as relatively black areas in each of the plots. UDs constructed using the fixed kernel least-squares cross-validation method for these data are illustrations in the lower row (sizes have been adjusted to provide visual correspondence—where precise estimates of the fits are given in Table 1).",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_102-PMC1797616-4-ponep0000207pg001.jpg"
} | 000488 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
|
{
"caption": "Electron Micrographs of TBE Virus at pH 8.0, 10.0, and 5.4TBE virus was preincubated at pH 8.0 (A), 10.0 (B), and 5.4 (C), fixed with formalin, and negatively stained by phosphotungstic acid adjusted to pH 8.0 (samples A and B) or pH 6.0 (sample C). Arrows in (B) point to the rough surface generated by alkaline pH and in (C) to the bulky spikes generated by low pH treatment. All micrographs have been recorded at the same magnification. In (B) and (C), the virions lost their shell-like icosahedral envelope structure, at least at the particle surface, and as a consequence display irregular shapes that give the impression that the virus diameter is smaller than in (A). However, in all cases, the core diameter of the best-preserved virions has a similar value. In (C), the virions are aggregated, a characteristic of TBE virus maintained at low pH.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_102-PMC1797619-0-ppatp0030020pg004.jpg"
} | 000489 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
|
{
"caption": "Electron Micrographs of TBE Virus Interacting with Liposomes at pH 5.4 and 10.0(A) Electron micrographs of a virus particle in the process of low pH–induced fusion with a liposome. Solid arrow points to low pH–induced projections at the virion surface; dotted arrow points to an electrodense structure presumed to be the nucleocapsid in the process of release.(B) Virus particles attached to liposomal membranes at alkaline pH. Negative stain by phosphotungstic acid adjusted to pH 8.0.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_102-PMC1797619-6-ppatp0030020pg007.jpg"
} | 000490 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
|
{
"caption": "OspF Is Associated with Attenuation of the Host Innate Immune ResponseThe images shown are of hematoxylin- and eosin-stained sections of lungs of mice 24 h after infection with wild-type or ΔospF Shigella or injection with equivalent volume of phosphate buffered saline.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_102-PMC1797620-12-ppatp0030021pg006.jpg"
} | 000491 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
|
{
"caption": "Viral Capsid Antigens in Myelin and Oligodendrocyte Cell Bodies during Persistent InfectionWild-type mice were inoculated intracranially with 106 PFU of TMEV. Spinal cords were dissected out 45 d p.i., snap-frozen, and 10-μm cryostat sections (longitudinal or transverse) were cut. The sections were stained for the oligodendrocyte marker CNPase (green) and for viral capsid antigens (red). The nuclei were stained with DAPI (blue). Viral antigens were found to colocalize to a large extent with CNPase.(A) Longitudinal section. The linear pattern of capsid antigen (arrows) corresponds most probably to virus replication in myelin sheaths (see B and C).(B) Capsid antigens in myelin sheath as seen in transverse sections (arrowhead).(C) Capsid antigen in the CNPase-positive cell body of an oligodendrocyte.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_102-PMC1797621-0-ppatp0030023pg005.jpg"
} | 000492 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
|
{
"caption": "Viral Antigens in the Brain of Wild-Type and shiverer MiceMice were inoculated intracranially with 106 PFU of TMEV. The brain was dissected out 5 d p.i., snap-frozen, and 10-μm cryostat sections were cut. Sections were stained with an anti-NeuN antibody (green) and an anti-TMEV capsid serum (red). Nuclei were stained with DAPI. (A–D) Wild-type mice. (E–H) shiverer mice. The virus was predominantly found in cortex (A and E), hippocampus (B and F), hypothalamus (C and G), and thalamus (not shown). The distribution was the same in wild-type and in shiverer mice. (D and H) Examples of infected neurons in, respectively, wild-type and shiverer mice. Optical sections were obtained with the Zeiss ApoTome device. wt, wild-type; shi, shiverer.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_102-PMC1797621-1-ppatp0030023pg002.jpg"
} | 000493 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
|
{
"caption": "Infection of Primary Cultures of Oligodendrocytes from Wild-Type or shiverer MiceCells were grown for 7 d with PDGF and 14 d without PDGF to induce oligodendrocyte differentiation. The cultures were infected with 500 PFU/cell of TMEV, fixed at different time points, and stained for CNPase, an oligodendrocyte marker (green) and viral capsid antigens (red).(A) Representative field of a wild-type culture 24 h p.i. White arrow, oligodendrocyte expressing viral capsid antigens; arrowhead, oligodendrocyte that did not express viral capsid; empty arrow, infected cell that was not an oligodendrocyte.(B) Percentage of oligodendrocytes that expressed viral capsid as a function of time p.i. There was no significant difference between wild-type and shiverer oligodendrocytes (Mann-Whitney test, p > 0.05, 18 and 24 h p.i.). wt, wild-type; shi, shiverer.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_102-PMC1797621-3-ppatp0030023pg004.jpg"
} | 000494 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
|
{
"caption": "TMEV Infection in the Optic TractWild-type mice were inoculated intravitreously with 106 PFU of TMEV. (A) Cartoon of the optical tract.(B) Cryostat sections of the retina stained for capsid antigens (red) and with DAPI (blue), 4 d p.i. The virus replicates in the retina, in particular in the ganglion cell layer (arrow). Insert: An infected retinal ganglion cell stained for NeuN (green) and viral capsid antigens (red).(C) TMEV capsid antigens (red) in a section of the ipsilateral optic nerve, 4 d p.i. The virus is present in glial cells along the nerve.(D) Coronal section of the brain of a wild-type mouse, 5 d p.i, stained for viral capsid antigens (red). The virus reached the LGN by axonal transport.(E–J) Optic nerve 4 d p.i. (E and H); Viral capsid antigens (red); (F) CNPase (green); (I) GFAP (green); (G) Merge of (E) and (F); and (J) Merge of (H) and (I). The virus infects oligodendrocytes and astrocytes.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_102-PMC1797621-7-ppatp0030023pg006.jpg"
} | 000495 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
|
{
"caption": "DENV-2 tropism for Aedes aegypti salivary glands. Salivary glands were dissected at the time points noted, fixed, and assayed for virus antigen by IFA (n = 30–50). Representative salivary glands (200×) from Chetumal and Rex-D mosquitoes at each time point are shown. DENV-2 virus antigen increased in the salivary glands through out the EIP. Negative controls (A and B) and salivary glands examined with light microscopy (C) are shown. In Rex-D mosquitoes, at 4 dpi viral antigen was typically associated with the fat body surrounding the salivary glands. In contrast salivary gland tissues of Chetumal mosquitoes contained viral antigen at 4 dpi.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_102-PMC1797809-0-1471-2180-7-9-5.jpg"
} | 000496 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
|
{
"caption": "DENV-2 antigen in the tracheal system of Chetumal mosquitoes following infection. DENV-2 antigen was detected by IFA in trachea of Chetumal mosquitoes at 2, 3, 4, 5, and 7 dpi (n= 40–50). Midgut- associated tracheal system (A) and uninfected trachea using light (B) and fluorescence (C) microscopy. Green arrows point to portions of DENV-2 infected trachea (E-L) (200×).",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_102-PMC1797809-3-1471-2180-7-9-4.jpg"
} | 000497 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
|
{
"caption": "DENV-2 tropisms in different tissues and organs of Aedes aegypti. DENV-2 viral E antigen distribution in different tissues and organs was revealed by IFA (green for FITC or Alexa 488) in Chetumal mosquitoes (n = 20). A) DENV-2 antigen in fat body of mosquito abdomen at 2 dpi. B) DENV-2 infected epithelial cells in midgut (4 dpi). C) DENV-2 is present in epithelial cells but absent in midgut-associated muscle (red phalloidin-Alexa® 546). D) DENV-2 in anterior midgut at 5 dpi. E) Infected esophagus at 7 dpi, dr.dv. dorsal diverticulum, car: cardia, oe: esophagus, and cr: crop. F) Hemocytes infected with DENV-2 at 10 dpi. G) Ommatidia of the compound eye exhibiting viral antigen at 12 dpi. H) Nervous system tissue profusely infected by DENV-2 at 14 dpi. I) Malphigian tubules expressing viral antigen (Mal. Tu.) at 16 dpi, il: ileum. These pictures represent the infection patterns observed. Original magnification was 200×, but pictures were cropped in Adobe Photoshop to improve presentation.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_102-PMC1797809-6-1471-2180-7-9-6.jpg"
} | 000498 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
|
{
"caption": "BAB Colocalizes with Chromatin Regulators on Polytene ChromosomesDouble immunostaining of polytene chromosomes with antibodies against BAB (green; B, D, F, H, J, and L), Corto (red; B, D, and F) and CRM (red; H, J, and L) in the cytological region of ddc (37C; A, B, G, and H), TH (65C; C, D, I, and J), and e (93C; E, F, K, and L). Colocalizations are indicated by yellow arrows. DAPI staining (blue) allows the visualization of euchromatic (lightly stained) and heterochromatic (densely stained) regions. Inverted black and white pictures of the DAPI staining are shown on the left to identify the cytological regions. Note the staining for BAB in the region of all three enzymes (B, D, F, H, J, and L), the colocalizations in the TH region with both Corto (D) and CRM (J), and in the ddc region with CRM (H).",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_102-PMC1797818-4-pgenp0030030pg007.jpg"
} | 000499 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
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